Device and method for bioassay of piercing-sucking pests

Abstract

The invention relates to a device and a method for bioassay of piercing-sucking pests. The device comprises a transparent bottle body and a detachable bottle cover, agarose gel is added at a bottle bottom, a ventilating hole is formed in a bottle wall of the transparent bottle body, a ventilating medical transparent adhesive tape covers the ventilating holes in a pasted manner from the inner side of the bottle wall, the bottle cover is provided with a food fetching port, a bottle cover film formed by sealing by a stretched paraffin sealing film from the outside of the bottle body is arranged on the food fetching port, liquid food is added on the bottle cover film, and a food protection film is adhered on the liquid food. By the device and the method, escape of test pests can be avoided while sufficient air can be provided for growing of the test pests; preparation of feed for bioassay can be completed by using the bottle cover only; agarose gel can maintain humidity, suitable for laodelphax striatellus, in the bottle body, bioassay experiments of the piercing-sucking pests can be completed in incubators which are good in temperature control effect, and mortality rate, floating above or below 20%, of a bioassay comparison treatment group in the past is lowered to be below 10%.

Description

technical field [0001] The invention relates to the field of biological control research, in particular to a bioassay device and a bioassay method for piercing-sucking pests. Background technique [0002] Piercing-sucking pests use needle-like mouthparts to feed on plant tissue juice, and cannot eat solid food. In the prior art, a bioassay method has been established for the feeding characteristics of piercing-sucking pests. The traditional bioassay system uses open ends at both ends. A glass tube with a film feeding method is used to place the feed at one end, and the other end is sealed with a nylon gauze and a rubber band to keep the air circulation in the tube. However, there are still defects in this method, which need to be improved urgently. This method has three obvious disadvantages. 1.) Use rubber bands to fix the nylon gauze at one end of the glass tube, which is very easy to produce gaps. After entering the gaps, the test insects will be trapped and die if they ...

AI Technical Summary

Problems solved by technology

This method has three obvious disadvantages. 1.) Use rubber bands to fix the nylon gauze at one end of the glass tube, which is very easy to produce gaps. After entering the gaps, the test insects will be trapped and die if they cannot escape, which will affect the accuracy of the bioassay results.

2.) When changing the feed, the process is fixed and uninterrupted, and the test insects are easy to escape, affecting the biotest results, and the operation is extremely cumbersome

3.) The air humidity in the test tube cannot be guaranteed

In northern China, the air is dry. Even if a constant temperature and humidity incubator is used, it cannot ensure that the small environment in the glass tube maintains a suitable humidity for the insects, which may easily cause the death of the test insects and affect the biotest results.

Due to the above problems, the death rate of the blank control test insects fluctuates around 20%, and when the death rate of the blank control is greater than 20%, the biometric data is invalid, resulting in waste of test insects and test proteins

Images