Device and method for bioassay of piercing-sucking pests

A piercing-sucking pest pest technology, applied in animal husbandry, etc., can solve the problems of easy escape of test insects, waste of test insects and test protein, fixed process, etc., to prevent escape of test insects, reduce escape of test insects, and facilitate operation Effect

Inactive Publication Date: 2015-08-19
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has three obvious disadvantages. 1.) Use rubber bands to fix the nylon gauze at one end of the glass tube, which is very easy to produce gaps. After entering the gaps, the test insects will be trapped and die if they cannot escape, which will affect the accuracy of the bioassay results.
2.) When changing the feed, the process is fixed and uninterrupted, and the test insects are easy to escape, affecting the biotest results, and the operation is extremely cumbersome
3.) The air humidity in the test tube cannot be guaranteed

Method used

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  • Device and method for bioassay of piercing-sucking pests
  • Device and method for bioassay of piercing-sucking pests
  • Device and method for bioassay of piercing-sucking pests

Examples

Experimental program
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Effect test

Example Embodiment

[0023] Example 1

[0024] Such as figure 1 , 2 As shown, the bioassay device for piercing and sucking pests includes a transparent bottle body 1 and a bottle cap 2 connected by screw threads. The transparent bottle body 1 has a volume of 50 mL, a bottle height of 69 mm, and a diameter of 42 mm. There is 4mL 2% agarose gel 9, the transparent bottle body 1 is provided with a 6mm diameter vent 3 on the bottle wall, and the vent 3 is covered with 3M breathable medical transparent tape 4 from the inside of the bottle wall. The bottle cap 2 is provided with a 30mm diameter feeding port 5, on which there is a stretched double-layer paraffin sealing film on the bottle cap film 6 formed by sealing from the outside of the bottle, and liquid food 7 is added to the bottle cap film 6 , The liquid food 7 has a food protective film 8 attached to it. The food protective film 8 is a stretched paraffin sealing film and is fixed on the bottle cap 2. Liquid food and feed sacs are wrapped in the pa...

Example Embodiment

[0025] Example 2 Bioassay method

[0026] a. Use transparent PET plastic bottles (volume 50mL, bottle height 69mm, diameter 42mm) for bioassay;

[0027] b. The white bottle cap is punched with a 30mm diameter hole opener; the side of the bottle body is opened with a 6mm drill bit, and 3M medical breathable tape is attached to the bottle

[0028] ( http: / / solutions.3m.com.cn / wps / portal / 3M / zh_CN / APACNexcare / Nexcare / Brand / tape / product2 / ), for the test insects to ventilate;

[0029] c. Add 4mL 2% agarose gel to the bottom of the bottle to moisturize;

[0030] d. Stretch the double-layer parafilm M, USA by 4 times and cover the open lid, and add 200μL of artificial feed;

[0031] e. Then use a stretched paraffin sealing film to cover the artificial feed and seal it to make a feed bag;

[0032] f. Put 20 test insects in each bottle, and repeat each treatment 3 times;

[0033] g. Cover the bottle with a black cloth, turn the mouth of the bottle toward the light source, and change the feed ever...

Example Embodiment

[0034] Example 3 Establishment and verification of bioassay system

[0035] The new biometric system is established on the basis of the original biometric system and has undergone many improvements. First, a plastic bottle is used, and a 6mm diameter hole is opened on one side of the bottle, and a breathable medical tape is attached to the bottle to seal the opening, which can prevent the test insect from escaping and provide sufficient amount for the growth of the test insect The air, which solves the impact of accidental death of test insects on bioassay results. Secondly, the bottle cap can be separated from the bottle body, and the preparation of feed for bioassay can be completed with only the bottle cap. Remove the feed to be replaced together with the cap and replace it with a new one. The whole process can be carried out in steps, which is convenient for operation, reduces the escape of test insects, and greatly facilitates the conduct of bioassay experiments. Finally,...

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Abstract

The invention relates to a device and a method for bioassay of piercing-sucking pests. The device comprises a transparent bottle body and a detachable bottle cover, agarose gel is added at a bottle bottom, a ventilating hole is formed in a bottle wall of the transparent bottle body, a ventilating medical transparent adhesive tape covers the ventilating holes in a pasted manner from the inner side of the bottle wall, the bottle cover is provided with a food fetching port, a bottle cover film formed by sealing by a stretched paraffin sealing film from the outside of the bottle body is arranged on the food fetching port, liquid food is added on the bottle cover film, and a food protection film is adhered on the liquid food. By the device and the method, escape of test pests can be avoided while sufficient air can be provided for growing of the test pests; preparation of feed for bioassay can be completed by using the bottle cover only; agarose gel can maintain humidity, suitable for laodelphax striatellus, in the bottle body, bioassay experiments of the piercing-sucking pests can be completed in incubators which are good in temperature control effect, and mortality rate, floating above or below 20%, of a bioassay comparison treatment group in the past is lowered to be below 10%.

Description

technical field [0001] The invention relates to the field of biological control research, in particular to a bioassay device and a bioassay method for piercing-sucking pests. Background technique [0002] Piercing-sucking pests use needle-like mouthparts to feed on plant tissue juice, and cannot eat solid food. In the prior art, a bioassay method has been established for the feeding characteristics of piercing-sucking pests. The traditional bioassay system uses open ends at both ends. A glass tube with a film feeding method is used to place the feed at one end, and the other end is sealed with a nylon gauze and a rubber band to keep the air circulation in the tube. However, there are still defects in this method, which need to be improved urgently. This method has three obvious disadvantages. 1.) Use rubber bands to fix the nylon gauze at one end of the glass tube, which is very easy to produce gaps. After entering the gaps, the test insects will be trapped and die if they ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 林克剑刘永磊耿丽丽李飞束长龙宋福平
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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