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Quick propagation method for akebia trifoliata stem

It is a fast and akebia technology, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc. It can solve the problems of difficult sterilization and achieve the effects of short growth cycle, robust growth, subculture multiplication and high rooting rate

Active Publication Date: 2015-08-26
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Problems solved by technology

[0003] The purpose of the present invention is to solve the difficulty in sterilizing explants of Akebia explants at present, and there are deficiencies in the technology of obtaining regenerated plants through tissue culture. The axillary buds are induced by body sterilization, with high induction rate, good multiplication effect and high rooting rate, which will increase the possibility of industrial seedling cultivation and genetic transformation in the future

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  • Quick propagation method for akebia trifoliata stem
  • Quick propagation method for akebia trifoliata stem
  • Quick propagation method for akebia trifoliata stem

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Embodiment 1

[0036] Do the following in order:

[0037] 1. Disinfection treatment of explants In the sunny morning between April and June 2014, select the semi-lignified stem sections of Akira japonica with moderate thickness, no pests and diseases, and take them back to the laboratory to remove the collected stem sections after spraying water. After the leaves, rinse with tap water for about 10 minutes, then rinse twice with an appropriate amount of detergent water, then rinse with tap water and place in an ultra-clean workbench, use 75% alcohol, 2% sodium hypochlorite solution and 0.1% HgCl 2 For disinfection, set different time gradients as shown in Table 1. During the disinfection process, use tweezers to stir constantly, rinse with sterile water 5-6 times, use sterile filter paper to blot the water on the surface of the stem section, cut off the browned part at both ends with a sterile blade, and inoculate the single-bud stem section into the culture medium. Statistical pollution rat...

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Abstract

The invention provides a quick propagation method for akebia trifoliata stem. The quick propagation method comprises such steps as explant disinfection, primary culture, multiplication culture, rooting culture, acclimatization and transplant. The quick propagation method has the advantages that the explant pollution rate is low, the propagation speed is high, and the culture cycle is short; not only is a way provided for quick propagation of elite akebia trifoliata stem plants, but also industrialized seedling culture of the akebia trifoliata stem and follow-up genetic system transformation become possible.

Description

technical field [0001] The invention belongs to the field of tissue culture of Akebiae, and in particular relates to a method for rapid propagation of Akebiae. Background technique [0002] White Akebia [Akebia trifoliata (Thunb.) Koidz.var.australis (Diels) Rehd.] is a semi-evergreen vine of the genus Akebia (Lardizabalaceae) in the Akebia family (Lardizabalaceae). It is a variant of Akebia trifoliata, mainly distributed in Jiangsu, Zhejiang, Jiangxi, Guangxi, Hunan, Hubei, Shanxi, Sichuan and other places (Jiang Yan et al. 2011; Liu Fang et al. 2004). The roots, vines and fruits of Baimutong can be used as medicine. It has weak gas, bitter and astringent taste, slightly cold in nature, and has diuretic, soothing liver and kidney, clearing heart and purging fire, dredging meridians and dissipating blood stasis, and anti-tumor effects (Gao Huimin et al. 2006 ). At present, the research on Akebia is mainly focused on the physical and chemical characteristics of oil, resourc...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李建安吴玲利雷小林马英孙颖柯镔峰
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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