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Compositions and methods of use

A kind of technology of composition, enzyme composition, applied in directions such as hydrolase

Inactive Publication Date: 2015-09-02
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, depending on the source microorganism of the mannanase, different β-mannanases may have different properties and activity profiles which may make them more suitable for one or more one industrial application and not suitable for other industrial applications

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  • Compositions and methods of use

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preparation example Construction

[0220] Preparation and use of replicable vectors

[0221] DNA encoding the Pkr Man3 protein or derivatives thereof (as described above) is prepared for insertion into an appropriate microorganism. According to the present compositions and methods, the DNA encoding a PkrMan3 polypeptide includes all DNA necessary to encode a protein having functional PkrMan3 activity. Thus, embodiments of the present compositions and methods include DNA encoding a PkrMan3 polypeptide derived from Paenibacillus species, including Paenibacillus species kribben.

[0222] The DNA encoding PkrMan3 can be prepared by constructing an expression vector carrying the DNA encoding PkrMan3. The expression vector carrying the inserted DNA segment encoding PkrMan3 can be any vector capable of autonomous replication in a given host organism or capable of integrating into the host's DNA, usually a plasmid, cosmid, virus particle or phage. Various vectors are publicly available. It is also envisioned that ...

example

[0280] The following examples are presented to provide one of ordinary skill in the art with a complete disclosure and description of how to make and use the present compositions and methods, and are not intended to limit the scope of what the inventors believe to be their inventive compositions and methods, nor are they intended to Experiments below performance were all or the only experiments performed. Efforts have been made to ensure accuracy with respect to numbers used (eg amounts, temperature, etc.), but some experimental errors and deviations should be accounted for.

example 1

[0282] Cloning of Glycosyl Hydrolase Pkr Man3 from Paenibacillus Cliburnum

[0283] Paenibacillus klibben was selected as a potential source of various glycosyl hydrolases and other enzymes for industrial applications. Genomic DNA for sequencing was obtained by first culturing a strain of Paenibacillus kribben on LB agar plates at 30°C for about 24 hours. Cellular material was scraped from the plates and genomic DNA was prepared using phenol / chloroform extraction. The genomic DNA was used for sequencing and for amplifying the pkr man3 gene for subsequent expression cloning.

[0284] Using a test performed by BaseClear (Leiden, The Netherlands) Sequencing by synthesis (SBS) technology (www.baseclear.com / sequencing / illumina-sequencing) sequenced the entire genome of Paenibacillus Cliburne, and the contigs (Contig) were passed through BioXpr (Namu, Belgium) Annotated by Namur, Belgium). Using BLASTP, one of the genes from the Paenibacillus Cliburnum genome sequence was fou...

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Abstract

The present compositions and methods relate to a beta-mannanase from Paenibacillus kribbensis, polynucleotides encoding the beta-mannanase, and methods of make and / or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and / or glucomannan (GM).

Description

[0001] Cross references to related patent applications [0002] This application claims priority to International Patent Application PCT / CN2012 / 086162 filed on December 7, 2012, which is incorporated herein by reference in its entirety. technical field [0003] The present compositions and methods relate to beta-mannanases derived from Paenibacillus kribbensis, polynucleotides encoding beta-mannanases, and methods for their production and use. Preparations containing recombinant β-mannanases have a wide variety of uses, for example for the hydrolysis of certain softwood types of lignocellulosic material and / or containing galactoglucomannan (GGM) and / or glucomannan (GM ) lignocellulosic biomass substrate. Background technique [0004] Cellulose and hemicellulose are the most abundant plant materials produced through photosynthesis. They can be degraded and used as an energy source by a variety of microorganisms (eg, bacteria, yeast, and fungi) that produce extracellular enz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24
Inventor L·华R·劳S·勒Z·钱Z·于
Owner DANISCO US INC
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