Microbial agent for fermenting Chinese herb residues
A technology for fermenting Chinese herbal medicines and microbial inoculants, applied in the field of microorganisms, can solve problems such as difficulty in meeting industrial production needs, long compost fermentation cycle, etc., and achieve the effects of accelerating water volatilization, increasing compost temperature, and promoting cellulose conversion
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Embodiment 1
[0039] Example 1 Microbial agent preparation
[0040] 1. Strain preparation medium:
[0041] Fungi, actinomycetes: sodium carboxymethylcellulose medium
[0042] Strain activation expansion medium:
[0043] Fungi: PDA medium; Actinomycetes: Gaoshi No. 1 medium.
[0044] 1.1 Carboxymethylcellulose sodium medium:
[0045] CMC-Na10g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 .7H 2 O0.5g, K 2 HPO 4 1.0g, NaCI0.5g, Congo red 0.05g, agar 20g, deionized water 1000mL, pH 7.0, sterilized at 121°C for 30min.
[0046] 1.2 Potato glucose medium:
[0047] Potato 200g, glucose 20g, tap water 1000ml, natural pH
[0048] Specific method: Wash and peel the potatoes, weigh 200g potatoes and cut them into small pieces, add water to boil, boil for 20-30 minutes, filter with eight layers of gauze, add glucose, heat and stir to mix, then add water to 1000ml after cooling down , divided into test tubes or Erlenmeyer flasks, stoppered, bandaged, (121°C) sterilized for about 20 minutes, then took out th...
Embodiment 2
[0057] Example 2 Aerobic composting test of Chinese herbal medicine slag
[0058] Aerobic composting was carried out on the fresh Chinese herbal medicine dregs with a total amount of more than 1 ton. The properties of the dregs are shown in Table 3. The composting effects of the inoculated group (JZ) and the control group (CK) were compared by adding microbial agents. The compost inoculation group and the control group were each set up in parallel. At the initial stage of composting, the total weight of each compost residue is about 275kg, and the moisture content is 72.11-74.22%. The length, width and height of the pile are 2m×0.8m×0.5m.
[0059] (1) Dosing of high-temperature bacterial agent: The high-temperature bacterial agent BY1 starts to be added on the first day of composting dregs, and the inoculum amount is about 0.2% (mass ratio). The cultured BY1 was concentrated by centrifugation. After the middle bran is sterilized, mix the bacteria solution and bran at a rat...
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