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Primer group for construction of mustard SSR genetic map and construction method of genetic map

A genetic map and primer set technology, applied in the field of plant molecular markers, can solve the problem of few SSR molecular markers

Inactive Publication Date: 2015-09-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the application of SSR molecular markers in mustard, and most of them use mustard for seeds, that is, rapeseed, as materials, and a few use mustard for seasoning as materials.
There is no report on the construction of the SSR genetic map using the unique vegetable mustard in my country as the material

Method used

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  • Primer group for construction of mustard SSR genetic map and construction method of genetic map
  • Primer group for construction of mustard SSR genetic map and construction method of genetic map
  • Primer group for construction of mustard SSR genetic map and construction method of genetic map

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Experimental program
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Effect test

Embodiment 1

[0245] 1. Material acquisition

[0246] The multi-year inbred line "Zhetong No. 1" with swollen stem was used as the female parent, and the non-swollen stem "Qiuai Daye Huangzhong Xuelihong" multi-year inbred line Tiller mustard leaf mustard was the male parent. Obtain F by conventional hybridization 1 , F 1 Obtain F by selfing 2 group. Randomly select F 2 200 strains in the population were used as the mapping population.

[0247] 2. DNA extraction and detection

[0248] (1) Preheat the CTAB extraction buffer with a mass-to-volume ratio of 0.02g / ml to 65°C in a water bath, and add β-mercaptoethanol before heating (the volume ratio of buffer to β-mercaptoethanol is 100:2 ).

[0249] (2) Pre-cool the mortar with liquid nitrogen, then fully grind the young leaves of the two parents, F1 and 200 F2 progeny under freezing conditions, and take the powder (about 1g) and quickly transfer it to the preheated CTAB containing 1mL In the sterile centrifuge tube of the extract, mix thoroughly an...

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Abstract

The invention belongs to the field of biotechnology, and relates to a primer group for the construction of a mustard SSR genetic map and a construction method of the genetic map. The primer group is an EST-SSR primer based on information development of hot pickled mustard tuber transcriptome sequence, and contains 112 pairs of polymorphic primers. A stem-inflated the ''Zhejiang Tong No.1'' multiple-year inbred line as a female parent and a stem-non-inflated ''Qiuai large-leaf yellow potherb mustard'' multiple-year inbred line tiller mustard as a male parent are subjected to hybridization to obtain F1 progeny; and F1 inbreeding is conducted to obtain a F2 mapping population. 112 pairs of polymorphic primers are used for the construction of the mustard genetic map through two parents and 200 F2 populations. The invention constructs the very first SSR genetic map of a mustard vegetable germplasm peculiar to our country and provides basis for further QTL mapping and marker-assisted breeding.

Description

Technical field [0001] The invention relates to plant molecular marker technology, in particular to a primer set for constructing a mustard SSR genetic map and a method for constructing the genetic map. Background technique [0002] Mustard (B.juncea) is an important cultivated crop of the Brassica genus of the cruciferous family. As a vegetable, oil and seasoning, this species is widely cultivated worldwide. Common mustard crops currently include mustard rape, condiment seed mustard, vegetable mustard and its varieties, etc., and the biology of different mustard crops is very different. Agronomic traits are controlled by multiple genes, and genetic improvement is more complicated. Early selection and prediction of mustard through molecular markers, the use of genetic maps to isolate excellent genes, and genetic improvement of complex traits controlled by multiple genes can improve the selection efficiency of target genes and accelerate the breeding process. [0003] At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 陈利萍李俊星邹晓霞曹丽雯于宁宁
Owner ZHEJIANG UNIV
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