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Method for preserving segregation population or distant hybirdization progeny of eggplant through stem microcuttage

A technology of distant hybridization and micro-cutting, which is applied in the fields of application, horticulture, botanical equipment and methods, etc., can solve the problems of easy variation of buds, influence on popularization and application, and long time, etc., and achieve simple steps and easy cultivation conditions Controlled, easy-to-manage effects

Inactive Publication Date: 2015-12-09
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can also preserve eggplant germplasm resources, the process is cumbersome and takes a long time, and the shoots obtained from callus redifferentiation are prone to variation, and more importantly, different genotypes have different effects on medium components and Hormone ratio has strong selectivity, which affects the popularization and application of this method

Method used

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  • Method for preserving segregation population or distant hybirdization progeny of eggplant through stem microcuttage
  • Method for preserving segregation population or distant hybirdization progeny of eggplant through stem microcuttage
  • Method for preserving segregation population or distant hybirdization progeny of eggplant through stem microcuttage

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Embodiment Construction

[0049] According to the technical scheme of the present invention, the specific implementation steps are:

[0050] 1. Determination of test materials

[0051] Select eggplant distant hybrid 11-435 (cultivated eggplant)×11-587 (wild eggplant) and its self-bred offspring F 2 as test material.

[0052] 2. Preparation of Media

[0053] The basic medium is MS medium, and the specific components are detailed in the list above.

[0054] Sprout meristem medium (cutting medium): MS basic medium + 0.1mg / LNAA + 1.0mg / L6-BA, 30g / L sucrose, 7g / L agar, add water to 1L, adjust pH to 5.8-6.0, in Sterilize at 121°C and 0.1Mpa for 20 minutes.

[0055] Sprout subculture and multiplication medium: MS basic medium+0.02mg / LNAA+1.0mg / L6-BA, 30g / L sucrose, 7g / L agar, add water to 1L, adjust pH to 5.8-6.0, at 121℃ , Sterilize for 20 minutes under the condition of 0.1Mpa.

[0056] Rooting medium: MS basic medium, 30g / L sucrose, 7g / L agar, add water to 1L, adjust pH to 5.8-6.0, sterilize at 121°C,...

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Abstract

The invention provides a method for preserving a segregation population or distant hybirdization progeny of an eggplant through stem microcuttage. The method comprises the following main steps: selecting a proper eggplant stem and carrying out sterilization; then carrying out cuttage on a bud mitogenetic medium, wherein buds successively grow out from leaf axil in 7 to 10 d; when the buds grow to 1 to 2 cm high, cutting the buds from positions near bases and sterilizing the cut buds; then transferring and inoculating the sterilized buds onto a subculture medium for culture; when the buds grow to 3 to 4 cm high, transferring the buds to a rooting medium for culture, wherein robust roots grow in 10 to 15 d and new eggplant seedlings are formed; and subjecting the new eggplant seedlings to domestication for a certain period of time and then planting the seedlings in a field, wherein the survival rate of the seedlings is 95 to 100%. If an F2 population is to be preserved for a long time, it only needs unintermittent transferring and inoculation onto the subculture medium once every 30 d. Compared with conventional cuttage and common tissue culture, the method provided by the invention has the following advantages: the method integrates the advantages of conventional cuttage and common tissue culture, i.e., short culture time, a small occupied land area, no invasion by insect diseases, easily controllable ambient conditions, convenience in management, and good stability and small possibility of variation due to generation of buds from stems. Thus, the method has critical application values in genetic and breeding research.

Description

technical field [0001] The invention relates to a method for preserving eggplant new germplasm and temporarily isolated populations, suitable for the preservation of germplasm resources that are difficult or impossible to preserve through sexual reproduction, especially temporary isolated populations for research or those with distant hybrid sterility or The preservation of non-fertile distant hybrid offspring, that is, the stem micro-cutting method, is a preservation method of asexual reproduction and belongs to the field of plant biotechnology. Background technique [0002] Micro-cutting preservation of eggplant germplasm resources has broad application prospects in the theoretical and practical research of crop genetics and breeding. Micro-cuttings can quickly and effectively regenerate eggplant germplasm resources that are difficult or impossible to preserve through sexual reproduction, and stabilize them. The preservation of the germplasm can not only solve the prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈雪平罗双霞张婷婷王星张巍巍申书兴
Owner HEBEI AGRICULTURAL UNIV.
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