Method for preserving segregation population or distant hybirdization progeny of eggplant through stem microcuttage

Abstract

The invention provides a method for preserving a segregation population or distant hybirdization progeny of an eggplant through stem microcuttage. The method comprises the following main steps: selecting a proper eggplant stem and carrying out sterilization; then carrying out cuttage on a bud mitogenetic medium, wherein buds successively grow out from leaf axil in 7 to 10 d; when the buds grow to 1 to 2 cm high, cutting the buds from positions near bases and sterilizing the cut buds; then transferring and inoculating the sterilized buds onto a subculture medium for culture; when the buds grow to 3 to 4 cm high, transferring the buds to a rooting medium for culture, wherein robust roots grow in 10 to 15 d and new eggplant seedlings are formed; and subjecting the new eggplant seedlings to domestication for a certain period of time and then planting the seedlings in a field, wherein the survival rate of the seedlings is 95 to 100%. If an F2 population is to be preserved for a long time, it only needs unintermittent transferring and inoculation onto the subculture medium once every 30 d. Compared with conventional cuttage and common tissue culture, the method provided by the invention has the following advantages: the method integrates the advantages of conventional cuttage and common tissue culture, i.e., short culture time, a small occupied land area, no invasion by insect diseases, easily controllable ambient conditions, convenience in management, and good stability and small possibility of variation due to generation of buds from stems. Thus, the method has critical application values in genetic and breeding research.

Description

technical field [0001] The invention relates to a method for preserving eggplant new germplasm and temporarily isolated populations, suitable for the preservation of germplasm resources that are difficult or impossible to preserve through sexual reproduction, especially temporary isolated populations for research or those with distant hybrid sterility or The preservation of non-fertile distant hybrid offspring, that is, the stem micro-cutting method, is a preservation method of asexual reproduction and belongs to the field of plant biotechnology. Background technique [0002] Micro-cutting preservation of eggplant germplasm resources has broad application prospects in the theoretical and practical research of crop genetics and breeding. Micro-cuttings can quickly and effectively regenerate eggplant germplasm resources that are difficult or impossible to preserve through sexual reproduction, and stabilize them. The preservation of the germplasm can not only solve the prob...

AI Technical Summary

Problems solved by technology

Although this method can also preserve eggplant germplasm resources, the process is cumbersome and takes a long time, and the shoots obtained from callus redifferentiation are prone to variation, and more importantly, different genotypes have different effects on medium components and Hormone ratio has strong selectivity, which affects the popularization and application of this method

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