Method for separated identification and fine positioning of rice agronomic trait gene by whole genome sequencing

A Whole Genome Sequencing, Fine Mapping Technology

Active Publication Date: 2017-03-08
CHINA NAT RICE RES INST
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Problems solved by technology

Traditional QTL mapping methods are time-consuming and labor-intensive, and have high requirements for polymorphism among materials

Method used

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  • Method for separated identification and fine positioning of rice agronomic trait gene by whole genome sequencing
  • Method for separated identification and fine positioning of rice agronomic trait gene by whole genome sequencing
  • Method for separated identification and fine positioning of rice agronomic trait gene by whole genome sequencing

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Experimental program
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Embodiment

[0024] Materials and Methods:

[0025] Rice material: Two groups of rice with different traits were used to validate the experimental method. One of them is the rice varieties Kuangsi Huaju (KSHJ, a large-grain indica variety) and Huazhan (HZ, a small-grain indica rice variety) with obvious differences in grain shape. Continuous backcrossing was carried out with Huazhan as the recurrent parent to obtain BC 4 f 1 A large single plant and a small single plant were selected from this group as sequencing samples; the other group consisted of the rice indica variety Fei B10 with long hairs on the leaf surface and the American rice variety Cypress with smooth leaves, and Cypress was used as the recurrent parent for continuous recurrent pay, get BC 4 f 1 Group, from which a single plant with hairy leaves on the surface and the recurrent parent Cypress were selected as sequencing samples. All the germplasm resources used in the present invention are provided by the germplasm resou...

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Abstract

The invention discloses a method for separated identification and fine positioning of a rice agronomic trait gene by whole genome sequencing; firstly, a 3-6 generation backcross population having separated target traits is obtained by backcross, two individual plants having the target trait with the biggest difference are selected for re-sequencing, heterozygous loci in sequencing results are compared and analyzed, and target trait candidate loci are screened; and then QTL separated identification and fine positioning are performed by using backcross F2 population linkage analysis. The method can quickly and accurately identify and finely position the rice QTLs, and lays a firm foundation for further cloning and functional analysis.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to a method for separating, identifying and finely positioning rice agronomic trait genes by using whole genome sequencing. Background technique [0002] In agricultural crops, many agronomic traits are regulated by one or more genes. In particular, some important agronomic traits are often regulated by several genes, which are called quantitative trait loci (quantitative trait loci, QTLs). QTLs also control many important rice traits, especially some yield-related agronomic traits. Therefore, the identification and isolation of QTLs has always been the focus of rice research. At present, many QTLs have been mapped in rice, but only a small part of them have been cloned, and the lack of efficient QTLs cloning methods is one of the major constraints. [0003] The conventional QTLs cloning method is mainly the map-based cloning method, first using the primary population (F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 倪深朱旭东肖文斐陈红旗王跃星
Owner CHINA NAT RICE RES INST
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