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The ssr molecular marker closely linked to the main gene locus of sesame resistance to stem spot blight and its application

A technology of molecular markers and major genes, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of fast chain decay of natural populations, achieve convenient and fast detection, and improve selection efficiency , targeted effects

Active Publication Date: 2019-12-24
OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex population background and the existence of subgroup structures, false positives are prone to occur, and the chain decay of natural populations is relatively fast. Therefore, high-density molecular markers are required to find sufficient variation in the population.

Method used

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  • The ssr molecular marker closely linked to the main gene locus of sesame resistance to stem spot blight and its application
  • The ssr molecular marker closely linked to the main gene locus of sesame resistance to stem spot blight and its application
  • The ssr molecular marker closely linked to the main gene locus of sesame resistance to stem spot blight and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Discovery of SSR molecular markers closely linked to genes related to sesame stem spot blight traits

[0056] (1) Construction of Recombinant Inbred Lines (RIL) Populations Resistant / Susceptible to Stem Blight and Identification of Stem Blight Resistance

[0057] The stem spot blight-resistant variety of sesame Zhongzhi 13 was used as the female parent to carry out hybridization with the sensitive germplasm "Dense Capsule Sesame" to obtain F1 seeds. F1 plants selfed to produce F2 generation seeds, F2 plants selfed to produce F3 generation seeds, and F3 generation Start planting by row and selfing to produce seeds. Each row only harvests the seeds of one individual plant, and plants one row of the next generation, and so on, and finally obtains the F7 generation segregation population, that is, the recombinant inbred line (RIL) groups;

[0058] Identify the stem point blight resistance of the parents and RIL lines, and calculate the disease index of the RIL i...

Embodiment 2

[0094] Example 2: Application of Molecular Markers Closely Linked to Sesame Sesame Disease-resistant Main Genes in Sesame Disease-resistant Breeding

[0095] 500 F2 individual plants were obtained by crossing Zhongzhi 13 with another stem-spot blight-sensitive germplasm "Added Sesame", and 500 F2 individual plants were planted because field identification of stem-spot blight resistance of F2 individual plants was not possible. The corresponding 500 F2:3 families were obtained from each plant, and the stem spot blight resistance of the F2:3 families represented the stem spot blight resistance of the F2 single plant. Molecular identification of F2 individual plants at the seedling stage, the specific steps include the extraction of the total DNA of the leaves (specifically as the DNA extraction method in Example 1) and the use of the main effect gene loci qCRR12.2 and qCRR3.2 for stem spot blight resistance Molecular identification of 4 pairs of molecular markers ZZM0913, ZZM375...

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Abstract

The invention provides an SSR (Simple Sequence Repeats) molecular marker closely linked to sesame stem rot resisting major genetic locus and applications thereof. Four SSR molecular markers closely linked to the sesame stem rot resisting major genetic locus are adopted and respectively named as ZMM0913, ZMM3752, ZMM5636 and ZMM5775; the primer sequences of the molecular markers respectively are: ZMM0913F:5'-CTCATGTGGAACGAGGCATA-3', ZMM0913R:5'-ATGGCCACCACCTAACATTC-3',ZMM3752F:5'-CAACGATGAGATGGCTTTGA-3',ZMM3752R:5'-TCTTGCACGCACAGTAGTCC-3',ZMM5636F:5'-CTGCTCATCACCTCTGGAAAG-3',ZMM5636R:5'-TGACCTATGATGTGATAACAGTTGG-3',ZMM5775F:5'-TTCACTTTGCTTTTGTTGCC-3', ZMM5775R:5'-GCCCATTCCATGAGTTTTTG-3'. Proved by F2 population verification, the four molecular markers: the ZZM0913, the ZZM3752, the ZZM5636 and the ZZM5775 are capable of increasing the efficiency of screening the stem rot resisting sesames, so that the molecular markers can be applied to the sesame stem rot resisting molecular marker-assisted selection breeding.

Description

technical field [0001] The invention belongs to the field of molecular markers, and in particular relates to an SSR molecular marker closely linked with the main gene site of sesame resistance to stem point blight and its application. Background technique [0002] Sesame is one of the four important oil crops in my country, and its planting is widely distributed. The Huanghuai and the middle and lower reaches of the Yangtze River are relatively concentrated, accounting for 70% of the national sesame area. The growth period of sesame is about 90 days. It is a temperature-loving crop and generally grows from June to August. The growth period of sesame in the main production areas is in the high temperature and rainy season, and high temperature and high humidity can easily induce stem point blight. Sesame stem point blight is also known as stem rot and charcoal rot, and its pathogen is Macrophomina phaseoli (Maubl.) Ashby.), which belongs to the fungus of the subphylum Deutero...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王林海张秀荣黎冬华张艳欣朱晓冬魏鑫朱晓凤
Owner OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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