Stool sample handling method and application thereof

A processing method and sample technology, applied in the field of biochemistry, can solve the problems of the effectiveness of medical means, inconvenient transportation, liquid leakage, etc., and achieve the effect of helping detection and treatment, simple instructions and use, and maintaining stability

Active Publication Date: 2015-09-23
HANGZHOU NEW HORIZON HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the stool samples used for disease detection are unstable and difficult to preserve, so the stool samples in hospitals or medical examination centers must be sent for testing immediately
This has caused certain inconvenience to the patients, and also increased the workload of the medical staff. At the same time, it has also increased the difficulty of the operation of the testing laboratory and the timely provision of results.
As a result, the proportion of patients who cooperate with providing samples has decreased, and the effectiveness of subsequent related medical methods has also been affected.
[0003] Although there have been research and development related protective fluids, the technology is complex, expensive, and inconvenient to transport, which may cause liquid leakage
At present, the main detection method is still on-site sampling and detection in hospitals. However, China's population base is too large, and hospital detection alone is far from being able to meet the increasing needs of Chinese people for health checkups.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Two groups of experiments are set, tube 1 and tube 2 are all sealable containers, wherein 5g of feces sample is added respectively in tube 1 and tube 2, (the said feces sample is detected as negative by fecal occult blood colloidal gold detection test paper (FOB)), and then Add 100ug / ml human hemoglobin to the two tubes respectively.

[0031] Add 12g of activated alumina desiccant (where the diameter of activated alumina is 1mm-2mm) into tube 1, and then seal the tube body; tube 2 is used as a control, without adding desiccant to seal the tube body. The above samples were stored at 25 degrees Celsius, and were tested by FOB on days 0, 1, 2, 3, 6, and 10, and the lowest detectable hemoglobin concentrations were recorded respectively.

[0032] Detection method: Take the sample in the tube for gradient dilution, record the maximum dilution factor, use this number to remove the original hemoglobin concentration of 100ug / ml, and obtain the minimum hemoglobin concentration in...

Embodiment 2

[0038] Set up two groups of experiments, tube 1 and tube 2 are both sealable containers, wherein tube 1 and tube 2 are respectively added with 5g of feces sample (the said feces sample is detected as negative by FOB), then add 100ug / ml into the two tubes respectively human hemoglobin.

[0039] Add 12g of activated alumina desiccant (where the diameter of activated alumina is 1mm-2mm) into tube 1, and then seal the tube body; tube 2 is used as a control, without adding desiccant to seal the tube body. The above samples were stored at 37 degrees Celsius, and were tested by FOB on the 0th, 1st, 2nd, 3rd, 6th, and 10th days, and the lowest detectable hemoglobin concentrations were recorded respectively.

[0040] Detection method: Take the sample in the tube for gradient dilution, record the maximum dilution factor, use this number to remove the original hemoglobin concentration of 100ug / ml, and obtain the minimum hemoglobin concentration in the feces that can be detected after pre...

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Abstract

The invention provides a stool sample handling method and application thereof. The stool sample handling method comprises the following steps: placing a stool sample to be detected in a sealable container; adding a drying agent into the container; sealing the container to enable the drying agent to absorb moisture in both the stool sample and air. The stool sample handling method can effectively maintain the stability of detected ingredients in the stool sample, enables self-help sample extraction by patients at home to be possible, and does not hinder downstream detection (immunologic detection); as a stool and drying agent mixture can directly enter the detection process, the flexibility of follow-up stool sample handling process is greatly facilitated, and the method is conductive to detection and treatment of digestive system diseases.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to a method for processing stool samples and an application thereof. Background technique [0002] At present, stool samples used for disease detection are unstable and difficult to preserve, so stool samples from hospitals or medical examination centers must be sent for testing immediately. This has caused certain inconvenience to patients, and also increased the workload of medical staff. At the same time, it has also increased the difficulty of operating and providing results in a timely manner for testing laboratories. As a result, the proportion of patients who cooperate in providing samples decreases, and the effectiveness of subsequent related medical methods is also affected. [0003] Although there have been research and development related protective fluids, the technology is complicated, the price is expensive, and the transportation is inconvenient, which may cause liquid leak...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 吕宁陈一友沈卫
Owner HANGZHOU NEW HORIZON HEALTH TECH CO LTD
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