Stool sample handling method and application thereof
A processing method and sample technology, applied in the field of biochemistry, can solve the problems of the effectiveness of medical means, inconvenient transportation, liquid leakage, etc., and achieve the effect of helping detection and treatment, simple instructions and use, and maintaining stability
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Embodiment 1
[0030] Two groups of experiments are set, tube 1 and tube 2 are all sealable containers, wherein 5g of feces sample is added respectively in tube 1 and tube 2, (the said feces sample is detected as negative by fecal occult blood colloidal gold detection test paper (FOB)), and then Add 100ug / ml human hemoglobin to the two tubes respectively.
[0031] Add 12g of activated alumina desiccant (where the diameter of activated alumina is 1mm-2mm) into tube 1, and then seal the tube body; tube 2 is used as a control, without adding desiccant to seal the tube body. The above samples were stored at 25 degrees Celsius, and were tested by FOB on days 0, 1, 2, 3, 6, and 10, and the lowest detectable hemoglobin concentrations were recorded respectively.
[0032] Detection method: Take the sample in the tube for gradient dilution, record the maximum dilution factor, use this number to remove the original hemoglobin concentration of 100ug / ml, and obtain the minimum hemoglobin concentration in...
Embodiment 2
[0038] Set up two groups of experiments, tube 1 and tube 2 are both sealable containers, wherein tube 1 and tube 2 are respectively added with 5g of feces sample (the said feces sample is detected as negative by FOB), then add 100ug / ml into the two tubes respectively human hemoglobin.
[0039] Add 12g of activated alumina desiccant (where the diameter of activated alumina is 1mm-2mm) into tube 1, and then seal the tube body; tube 2 is used as a control, without adding desiccant to seal the tube body. The above samples were stored at 37 degrees Celsius, and were tested by FOB on the 0th, 1st, 2nd, 3rd, 6th, and 10th days, and the lowest detectable hemoglobin concentrations were recorded respectively.
[0040] Detection method: Take the sample in the tube for gradient dilution, record the maximum dilution factor, use this number to remove the original hemoglobin concentration of 100ug / ml, and obtain the minimum hemoglobin concentration in the feces that can be detected after pre...
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