Preparation method of dressing skin

A technology for leather and raw materials, which is applied in the field of virus inactivation of animal-derived biological dressings, can solve the problem of low virus inactivation efficiency, and achieve the effects of improved virus inactivation efficiency, low cost and improved safety.

Inactive Publication Date: 2015-09-30
CHANGSHA DARUIQI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to address the problem of low virus inactivation efficiency and provide a method for preparing dressings with high virus inactivation efficiency and safety

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] The preparation method of the dressing skin of an embodiment, comprises the following steps:

[0022] S100: Depilate the heterogeneous leather material, scrape off the fat and cuticle, and obtain the heterogeneous leather material with a thickness of 0.3-0.5 mm.

[0023] Preferably, the thickness of the different leather material is 0.3mm.

[0024] Preferably, the heterogeneous skin raw material is pigskin raw material, and pigskin is similar to human skin in structure, and it can better adhere to the wound surface, play a role in reducing water evaporation of the wound surface, reducing electrolyte loss, blocking bacteria and controlling infection. The clinical effect is good, and it is a better alternative skin allograft, which has been widely used in the field of burns and scalds.

[0025] As a wound dressing skin of animal origin, pigskin must be strictly controlled for its viral load. Therefore, the choice of virus inactivation process is very important. Accordi...

Embodiment 1

[0056] Depilate the heterogeneous leather raw material, scrape off the fat and cuticle, and obtain the heterogeneous leather material with a thickness of 0.3mm, soak the heterogeneous leather material in 1mol / L NaCl solution at room temperature for 20h, wash with sterile water, and wash with 37℃ Soak in 0.35% neutral protease solution for 4 hours, and then wash with sterile water to obtain xenograft skin.

[0057] Put the xenograft in a shaker with an oscillation frequency of 100 rpm. Control the temperature to 60° C. in a constant temperature water bath, and heat for 120 minutes to obtain the virus-inactivated heterogeneous skin. The heterogeneous skin that has been preliminarily inactivated by the virus is irradiated with gamma rays with an irradiation dose of 25 kGy to obtain the dressing skin.

Embodiment 2

[0059] Depilate the heterogeneous leather raw material, scrape off the fat and cuticle, and obtain the heterogeneous leather material with a thickness of 0.5mm. Soak the heterogeneous leather material in 2mol / L NaCl solution at room temperature for 12h, wash with sterile water, and wash with 37℃ Soak in 0.5% neutral protease solution for 6 hours, and then wash with sterile water to obtain xenograft skin.

[0060] Put the xenograft in a shaker, and the shaking frequency of the shaker is 110rpm. Control the temperature to 55° C. in a constant temperature water bath, and heat for 90 minutes to obtain the heterogeneous skin with the virus initially inactivated. The heterogeneous skin that has been preliminarily inactivated by the virus is irradiated with gamma rays with an irradiation dose of 15 kGy to obtain the dressing skin.

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Abstract

The invention discloses a preparation method of a dressing skin. The preparation method comprises the following steps of: removing hairs of xenoskin raw materials, and scraping to remove fat and a cuticle so as to obtain a xenoskin material with the thickness of 0.3-0.5 mm; sequentially immersing by using NaCl solution, cleaning by using sterile water, immersing by using neutral protease with the mass fraction of 0.2-0.5% at 37 DEG C, and cleaning by using sterile water so as to obtain a xenoskin; and inactivating the xenoskin so as to obtain the dressing skin, wherein the inactivating step comprises the steps of heating the xenoskin at 50-60 DEG C for 60-120 MIN and irradiating the xenoskin by using gamma rays. The preparation method of the dressing skin is simple to operate and low in cost; four kinds of viruses including PRV (Pseudorabies Virus), VSV (Vesicular Stomatitis Virus), PV1 (Poliovirus 1) and PPV (Porcine Parvovirus) can be completely inactivated; the virus inactivating efficiency is obviously increased; the problem that PV1 cannot be completely inactivated only by irradiating gamma rays can be solved; and therefore, the safety of the dressing skin is increased.

Description

technical field [0001] The invention relates to the technical field of animal-derived biological dressing virus inactivation, in particular to a preparation method of dressing skin. Background technique [0002] Existing heterogeneous skins used for burns, thermal injuries or chemical injuries are often pigskin. The structure of pigskin is similar to that of human skin. It can better adhere to the wound surface, reduce the evaporation of wound surface water, reduce electrolyte loss, block bacteria and control infection. It has good clinical effect and is a good alternative Alternative skin for selection has been widely used in the fields of burns and scalds. As a wound dressing skin of animal origin, pigskin must be strictly controlled for its viral load. Therefore, the choice of virus inactivation process is very important. At present, pigskin for burns and scalds is mainly irradiated with gamma rays (gamma rays) to inactivate viruses. [0003] According to the provisio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40
Inventor 周天行
Owner CHANGSHA DARUIQI IND
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