Cadmium-chelated immune complex as well as preparation method and application thereof
An immune complex, chelating technology, applied in the medical field, can solve the problems of body damage, functional changes, deficiency and so on
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[0059] Wherein, the preparation method includes:
[0060] A) the step of synthesizing cadmium chelated immune complex;
[0061] B) The step of purifying the cadmium chelating immune complex: using immunoaffinity chromatography to remove the carrier protein, specific antibody and Cd that did not participate in the reaction in the cadmium chelating immune complex synthesized in step A) 2+ .
[0062] Specifically, in the preparation method of the cadmium chelated immune complex,
[0063] Described step A) concrete steps are as follows:
[0064] (1) prepare chelating agent: chelating agent is dissolved in solvent, is mixed with chelating agent solution;
[0065] (2) Prepare the carrier protein solution: add the carrier protein to the borate solution;
[0066] (3) Stirring overnight: adding the chelating agent solution in step (1) to the carrier protein solution in step (2), stirring in a shaker overnight to obtain a mixed solution;
[0067] (4) Dialysis bag pretreatment: add ...
Embodiment 1
[0165] The reagents used in this embodiment are as follows:
[0166] Borate buffer solution, the method is as follows: Weigh 0.31g of boric acid and dissolve it in 400ml of ultrapure water, adjust the pH to 9.0 with 0.1M NaOH aqueous solution, and set the volume to 500mL to obtain the solution;
[0167] Hemoglobin solution, the preparation method of which is as follows: take hemoglobin and dissolve it in the borate buffer solution with a molar mass of 0.01M and a pH of 9.0, fully oscillate and dissolve, and prepare a hemoglobin solution of 1.0 mg / mL;
[0168] 5mmol / L EDTA+200mmol / LNaHCO 3 Solution, 5mmol / L EDTA+200mmol / LNaHCO per 1000ml 3 The solution is prepared by the following method: Take 1.86g EDTA 2H 2 O, 16.8g NaHCO 3 , add 900mL of ultrapure water, then adjust the pH to 8.0 with a NaOH aqueous solution with a molar mass of 1.0M, then add ultrapure water to make the volume to 1000ml, autoclave, and store at room temperature to obtain the product;
[0169] ITCBE, pur...
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