Micro-fluidic chip used for simultaneous detection of bladder cancer cells, calculi, blood cells, and bacteria in urine

Abstract

The invention belongs to the technical field of cell detection apparatus, and relates to a micro-fluidic chip used for simultaneous detection of bladder cancer cells, calculi, blood cells, and bacteria in urine. The micro-fluidic chip is composed of a lower sample channel layer and an upper pneumatic microvalve layer used for controlling on-off of sample channels; the lower sample channel layer is composed of an urine sample inlet, an urine sample main channel, and an urine sample outlet liquid storage tank; a plurality of detecting units, including an urinary calculi interception unit, an urinary bladder transitional epithelial cancer detection unit, an urinary bladder adenocarcinoma cell detection unit, an urinary bladder squamous carcinoma cell detection unit, a blood cell detection unit, and a bacterium detection unit , are arranged on the urine sample main channel; and the detecting units are connected in series, and are arranged in a circular symmetry manner. The micro-fluidic chip is high in specificity and sensitivity, can be used for determining that whether a patient suffers from bladder cancer and urinary tract symptoms just via detection on urine, and is a cheap high-efficiency urine detection tool.

Description

technical field [0001] The invention belongs to the technical field of cell detection devices, and is a detection device for detecting bladder cancer cells in human urine samples based on the principle of antigen-antibody immunoadsorption reaction, microfluidic technology and protein microarray technology, and can detect urinary tract infection at the same time , urinary tract bleeding and stones. Background technique [0002] Bladder cancer is the most common malignant tumor of the urinary system. The main means of screening for initial diagnosis with urinary tract symptoms and re-examination for patients after bladder cancer surgery include urine exfoliation cytology, cystoscopy and fluorescent in situ hybridization (FISH). hybridization, FISH) technology. These methods all have certain disadvantages: [0003] 1. Although urine exfoliation cytology is widely used, the false positive rate is high; [0004] 2. Although cystoscopy is the "gold standard" of clinical testing...

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Problems solved by technology

[0003] 1. Although urine exfoliation cytology is widely used, the false positive rate is high;

[0004] 2. Although cystoscopy is the "gold standard" of clinical testing, it is an invasive test, and the patient has to suffer a lot; and the tumor entity can only be accurately detected when it grows to a certain size, which is not conducive to early diagnosis;

[0005] 3. FISH technology has high specificity and sensitivity, but the detection cost is expensive, and it still needs to cooperate with microscopic examination to confirm the diagnosis

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