The invention belongs to the technical field of cell detection apparatus, and relates to a micro-fluidic chip used for simultaneous detection of bladder cancer cells, calculi, blood cells, and bacteria in urine. The micro-fluidic chip is composed of a lower sample channel layer and an upper pneumatic microvalve layer used for controlling on-off of sample channels; the lower sample channel layer is composed of an urine sample inlet, an urine sample main channel, and an urine sample outlet liquid storage tank; a plurality of detecting units, including an urinary calculi interception unit, an urinary bladder transitional epithelial cancer detection unit, an urinary bladder adenocarcinoma cell detection unit, an urinary bladder squamous carcinoma cell detection unit, a blood cell detection unit, and a bacterium detection unit , are arranged on the urine sample main channel; and the detecting units are connected in series, and are arranged in a circular symmetry manner. The micro-fluidic chip is high in specificity and sensitivity, can be used for determining that whether a patient suffers from bladder cancer and urinary tract symptoms just via detection on urine, and is a cheap high-efficiency urine detection tool.