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Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain

A strain and resistance technology, which is applied in the rhizosphere sedum chromium-resistant strains, screening and application fields, can solve the problems of plant growth, animal development and human health hazards

Inactive Publication Date: 2015-10-14
CHINA THREE GORGES CORPORATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chromium pollution has the characteristics of potential, concealment and long-term nature. Once the water body and soil are polluted, it is difficult to restore it even if manpower and material resources are invested, and it is very harmful to plant growth, animal development and human health.

Method used

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  • Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain
  • Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain
  • Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Separation of Sedum rhizosphere chromium resistant strain Bacillus pumilus JCr9

[0030] Materials and Methods

[0031] 1. Medium

[0032] Bacterial culture medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0.

[0033] Preparation of screening medium: the basic medium is 1000ml of bacterial medium, add 10ml of trace element solution and vitamin solution, sterilize at 121℃ for 20min, cool to about 70℃, add chromium solution mother liquor to make the chromium content in the medium 1000mg· L -1 . Incubate at 37°C.

[0034] The trace element liquid is: MnSO 4 0.01g, ZnSO 4 0.05g, H 3 BO 3 0.01g, CaCl 2 0.01g, dilute to 1L, store at 4°C in the dark. The vitamin solution is: creatine 0.025g, ascorbic acid 0.025g, riboflavin 0.025g, citric acid 0.02g, dilute to 1L, and store at 4°C in the dark. Mother liquor of chromium solution: K 2 CrO 4 Formulated into Cr 6+ The concentration is 200mg·mL -1 After filtering and sterilizing the mother liquor, stor...

Embodiment 2

[0037] Example 2 Identification of strain JCr9

[0038] 1. Genomic DNA extraction

[0039] The above-mentioned strain JCr9 was cultivated in large quantities according to conventional technical means, and then its genomic DNA was obtained.

[0040] 2. Identification method of 16S rDNA of strain JCr9

[0041] 2.1 PCR amplification and sequencing of 16S rDNA of strain JCr9 and construction of phylogenetic tree

[0042] 2.1.1 PCR amplification of 16S rDNA gene sequence

[0043] The primers at both ends of the 16S rDNA gene sequence use universal primers: forward primer BSF8 / 20: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID NO: 1) and reverse primer BSR1541 / 20: 5'-AAGGAGGTGATCCAGCCGCA-3' (SEQ ID NO: 2). The PCR reaction system was 50μL, and the reaction conditions were denaturation at 94°C for 5min; then 35 cycles of reaction: denaturation at 94°C for 45s, annealing at 50°C for 45s, extension at 72°C for 90s; then extension at 72°C for 10min, and finally stored at 4°C.

[0044] 2.1.2 Cloning and seque...

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Abstract

The invention relates to a chromium-resisting bacillus separated from rhizosphere of red-spotted stonecrop (pumilus bacillus pumilus JCr9) which is preserved in China General Microbiological Culture Collection Center (CGMCC) on Dec, 1, 2014, with the preservation number of CGMCC No.10078, wherein the 16S rDNA gene sequence is shown in figure 1. The pumilus bacillus pumilus JCr9 is gram-negative bacterium and aerobic corynebacterium, has no atrichia or capsule, has strong tolerance for heavy metal chromium, and has practical significance and important engineering application value for plant-microbial remediation of soil polluted by heavy metal chromium.

Description

Technical field [0001] The invention relates to a chromium-resistant Bacillus pumilus JCr9 isolated from the rhizosphere of sedum and its application in plant-microbe joint repair of chromium heavy metal contaminated soil. Background technique [0002] In recent years, with the development of industry, chromium compounds have been widely used in industry. The trivalent and hexavalent chromium compounds produced are discharged into the natural environment through waste water, waste residue, chromium-containing dust, etc., causing serious damage to the soil, water and atmosphere. Chromium pollution. [0003] my country’s chromium pollution is also very serious. The area of ​​farmland contaminated by heavy metals is 20 million hectares. 2 , Causing about 20 billion yuan in economic losses every year. According to statistics, about 600,000 tons of chromium slag is discharged from factories in my country each year, and the accumulated storage of chromium slag over the years has reached...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/07
CPCB09C1/10C12N1/20C12N1/205C12R2001/07
Inventor 林海龙范阳冯晶石田宇哲李德斌
Owner CHINA THREE GORGES CORPORATION
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