Method for tissue culture and rapid propagation of strobilanthes cusia
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A technology of horse blue group and culture temperature, applied in the field of plant tissue culture, to achieve the effect of long reproduction cycle and low reproduction rate
Inactive Publication Date: 2015-10-21
刘祖英
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At present, there are few reports on the research on the tissue culture of S. indigo. The present invention uses the stem section of S. indigo with axillary buds as explants, and establishes the establishment of S. indigo through steps such as bud induction, multiplication, strong seedlings, rooting, hardening and transplanting. Indigo tissue culture rapid propagation technology system provides technical support for further industrial seedling cultivation in indigo tissue culture, and lays the foundation for the genetic transformation and variety improvement of indigo in the future
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Embodiment 1
[0018] (1) Induction culture: Take the stems of the axillary buds that were born in the current year as explants, wash off the surface attachments with clean water, soak in laundry detergent for 5 minutes, then rinse with running water for 1 hour, and disinfect with 75% ethanol for 40 seconds in an ultra-clean workbench Wash 4 times with sterile water, then sterilize with 0.1% mercuric chloride solution (plus 2-3 drops of Tween) for 11 minutes, rinse 4 times with sterile water, absorb water with sterile filter paper, and inoculate into induction medium for clustering Bud induction culture. After inoculation, they were first cultured in total darkness at 25°C for 7 days, and then placed in light for 12 hours a day with a light intensity of 2500 lx and cultured at a culture temperature of 25°C. The average initiation speed was 6.71 days, and the explant bud induction rate reached 97.66%. The induction medium is: MS+1.5mg / LKT+0.01mg / LIBA+27g / L sucrose+4.5g / L agar, with a pH ...
Embodiment 2
[0024] (1) Induction culture: take the stems of the axillary buds that were born in the current year as explants, wash off the surface attachments with water, soak in laundry detergent for 10 minutes, then rinse with running water for 3 hours, and disinfect with 75% ethanol for 60 seconds in an ultra-clean workbench Wash 5 times with sterile water, then sterilize with 0.1% mercuric chloride solution (plus 2-3 drops of Tween) for 15 minutes, rinse 5 times with sterile water, absorb water with sterile filter paper, and inoculate into induction medium for clustering Bud induction culture. After inoculation, they were first cultured in total darkness at 27°C for 10 days, and then placed in light for 14 hours a day with a light intensity of 3000 lx and cultured at a culture temperature of 27°C. The average initiation speed was 7.24 days, and the bud induction rate of explants reached 100%. The induction medium is: MS+2.0mg / LKT+0.1mg / LIBA+35g / L sucrose+4.5g / L agar, with a pH of...
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Abstract
The invention discloses a method for tissue culture and rapid propagation of strobilanthes cusia. The strobilanthes cusia belongs to perennial herbaceous plants of strobilanthes of acanthaceae. The rhizome of the strobilanthes cusia can be used as medicine and has a very good treatment effect in clinical application. Cutting propagation and a traditional seed propagation manner are adopted as the main propagation manners of the strobilanthes cusia at present; however, the propagation methods are low in propagation rate and long in propagation period and cannot meet the requirement for mass production. According to the method for tissue culture and rapid propagation of the strobilanthes cusia, the stem with axillary buds of strobilanthes cusia serves as an explant, a technical system for tissue culture and rapid propagation of the strobilanthes cusia is established through the steps of bud induction, multiplication, seedling strengthening, rooting, acclimatizing, transplanting and the like, a technical support is provided for further industrial seedling culture of the strobilanthes cusia tissue culture, and a foundation is laid for genetic transformation, breed improvement and the like of the strobilanthes cusia in the future.
Description
technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of horse indigo. Background technique [0002] Ma Lan ( Strobilanthes cusia ), also known as Nanbanlan, Daqing, Shanqing, Shanlan, Indigo Daisy, Indigo, Indigo Leaf, etc., are perennial herbaceous plants belonging to the genus Equus in the Acanthaceae family. Malan is distributed in Zhejiang, Jiangxi, Hunan, Fujian, Guangdong, Guangxi, Sichuan, Yunnan and other provinces and regions. The distribution of its wild resources is relatively scattered, and the distribution of plant communities is sporadic, and there is a danger of gradually disappearing. The whole plant of Malan has high medicinal value, rich in alkaloids, lignin, pentacyclic triterpenes, quinazolones and other compounds, which have anti-inflammatory, anti-viral, anti-tumor, antibacterial, and liver-protecting functions. And it has...
Claims
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