Inhibitor of panicle germination and method for inhibiting rice panicle germination
An inhibitor, rice technology, applied in the field of panicle germination inhibitors and inhibition of rice panicle germination, can solve problems that need to be further improved, achieve good safety and environmental protection benefits, maintain comprehensive quality, and the effect of simple and convenient methods
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Embodiment 1
[0025] 1. Test material: F1 seeds of hybrid rice Qianyou 1.
[0026] 2. Drugs for testing: panicle germination inhibitors A, G, I.
[0027] 3. Test method:
[0028] Drug preparation and seed treatment: The three drugs were prepared according to Table 1 to the concentration required for the experiment. After the rice seeds were sterilized by 0.1% sodium hypochlorite solution, they were evenly sprayed with clear water (CK) and panicle germination inhibitor respectively, and the seeds were taken out and dried after one hour.
[0029] Germination conditions and index determination: the germination conditions were set at 30°C for 8 hours with light; and 20°C for 16 hours without light. Use a paper bed for germination, a small germination box (12*12cm) plus three layers of filter paper. There were 50 seeds per treatment, and three replicates were set up. The germination potential was measured on the third day of germination, and the daily germination number was counted in the fi...
Embodiment 2
[0042] 1. Test material: F1 seeds of hybrid rice Qianyou 1.
[0043] 2, test agent: panicle germination inhibitor A, G, I (with embodiment 1).
[0044] 3. Test method:
[0045] Medicament preparation and seed treatment: with embodiment 1.
[0046] Simulation of panicle germination conditions: In the light incubator, set the light and temperature conditions as 8 hours of light, 38°C; 16 hours of no light, 20°C. Place an air humidifier on the bottom layer of the light incubator to keep the average humidity of the incubator at about 92%. Each treatment was repeated three times, and 50 seeds of each repetition were suspended on a nylon mesh cloth, placed in an incubator for several days, and then the ear germination rate and ear germination index were counted.
[0047] 4. Grading standards for ear germination seeds and calculation methods for ear germination indicators:
[0048] Level 0 - full seed;
[0049] Grade 1-seeds with ruptured lemma shell;
[0050] Level 2 - the sho...
Embodiment 3
[0057] In Examples 1 and 2, the effect of the ear germination inhibitor on delaying seed germination was confirmed. In this example, the field ear germination inhibitory effect of the ear germination inhibitor was studied.
[0058]1. Test material: female parent of indica three-line hybrid rice Qianyou 1.
[0059] 2, test agent: panicle germination inhibitor A, G, I (with embodiment 1).
[0060] 3. Test method:
[0061] The experiment was carried out at the farm of Zijingang Campus of Zhejiang University in 2014. According to the characteristics of the growth period of the parents, the seeds were sown in stages, and gibberellin was sprayed in stages to ensure that the parents met at the flowering stage. Three repetitions were set for each treatment. The length of a single plot in the north-south direction was about 2.5m with an interval of 0.5m, the width in the east-west direction was about 1m, and the interval between the male parents was 20cm. The area of the plot was 2....
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