Newcastle disease and avian flu antigen-antibody complex inactivated vaccine and preparation method

An antigen antibody and Newcastle disease technology, applied in the direction of antibodies, virus antigen components, antibody medical components, etc., can solve the problems of insufficient passive immunity, low yolk antibody titer, and increased vaccine production costs, so as to shorten the blank period of immunity , high purity, and the effect of improving immune protection

Active Publication Date: 2015-10-21
兆丰华生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that its yolk antibody titer is not high, and the dose is not enough to provide strong passive immunity
In addition, adding egg yolk antibody to the vaccine directly increases the production cost of the vaccine, the economic value of broiler chickens is not high, and expensive vaccines are difficult to receive

Method used

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  • Newcastle disease and avian flu antigen-antibody complex inactivated vaccine and preparation method
  • Newcastle disease and avian flu antigen-antibody complex inactivated vaccine and preparation method
  • Newcastle disease and avian flu antigen-antibody complex inactivated vaccine and preparation method

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preparation example Construction

[0018] The preparation method of the present invention comprises the following steps:

[0019] 1) Prepare avian influenza antigen: clean and disinfect non-immune chicken embryos, inoculate H9N2 subtype HP strain avian influenza virus in its allantoic cavity after hatching, then continue to hatch, harvest chicken embryo liquid under aseptic conditions, and carry out Purify and concentrate to obtain new chicken embryo fluid, and the new chicken embryo fluid meets the content of avian influenza virus ≥ 10 8.7 EID 50 / 0.1mL, then inactivate the newly obtained chicken embryo liquid with formaldehyde;

[0020] 2) Preparation of Newcastle disease inactivated antigen and avian influenza inactivated yolk antibody: firstly use the H9N2 subtype HP strain avian influenza virus inactivated vaccine to immunize chicken breeders, detect the level of avian influenza antibody in chicken embryos, and select the titer to reach 9log2 or more Then, the selected high-immunity eggs of avian influen...

Embodiment 1

[0028] 1. Preparation of chicken embryos free from avian influenza:

[0029] (1) Immunization program for breeder chickens: the antibody is the egg yolk antibody obtained after immunization with the H9N2 subtype avian influenza HP strain inactivated vaccine. First, use 2 times the dose of inactivated vaccine to immunize the chicken embryos; after 7 to 10 days, use 2 to 5 times the dose to boost the chicken embryos; after 10 days, use 2 to 5 times the dose to immunize the chickens embryos for maintenance immunity.

[0030] (2) Harvesting of high-immune chicken embryos: HI was detected every 3 days after maintaining immunization, and when the HI value was above 9log2, chicken embryos were collected for the preparation of egg yolk antibody and Newcastle disease antigen.

[0031] 2. Preparation of Newcastle disease antigen:

[0032] (1) Disinfection: the collected bird flu-free chicken embryos are cleaned and disinfected with 0.1% bromogeramine solution, put into an incubator at...

Embodiment 2

[0083] 1. Preparation of chicken embryos free from avian influenza:

[0084] (1) Immunization procedure for chicken embryos: the antibody is the egg yolk antibody obtained after immunization with the HP strain inactivated vaccine. First, immunize the breeder chickens with 2 times the immunization dose of the inactivated H9N2 subtype HP strain vaccine; 7-10 days later, use 2-5 times the immunization dose to boost the immunization; Doubling immunization dose for maintenance immunization of breeder chickens.

[0085] (2) Harvesting of high-immune chicken embryos: HI was detected every 3 days after maintaining immunization, and when the HI value was above 9log2, chicken embryos were collected for the preparation of egg yolk antibody and Newcastle disease antigen.

[0086] 2. Preparation of Newcastle disease antigen:

[0087] (1) Disinfection: the collected bird flu-free chicken embryos are cleaned and disinfected with 0.1% bromogeramine solution, put into an incubator at 37° C. ...

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Abstract

The present invention relates to a Newcastle disease and avian flu antigen-antibody complex vaccine preparation method, which comprises: preparing avian flu inactivated antigen, preparing Newcastle disease inactivated antigen and avian flu inactivated yolk antibody, respectively emulsifying the inactivated Newcastle disease antigen, the inactivated avian flu antigen and the inactivated avian flu yolk antibody semi-finished product, and mixing. According to the present invention, the Newcastle disease and avian flu antigen-antibody complex vaccine with characteristics of vaccine immunization protection capability enhancing, antigen release delaying, good safety, high purity and production cost saving, suitable for all chicken flocks and especially providing safety and effectiveness for chicks and chicken having diseases is provided.

Description

technical field [0001] The invention belongs to the field of new biological medicine, and relates to a Newcastle disease and avian influenza antigen-antibody complex vaccine and a preparation method thereof. technical background [0002] Newcastle Disease (ND) is an acute and highly contagious infectious disease of chickens and various poultry caused by Newcastle Disease Virus (NDV), with high morbidity and mortality. Avian Influenza (AI) is caused by type A influenza virus and is an acute infectious disease that occurs in various poultry and wild birds. The type A influenza virus that causes avian influenza is called avian influenza virus (Avian Influenza Virus, AIV). Once, it brought a heavy blow to the poultry industry. [0003] At present, the main means of prevention and treatment of ND and AI is vaccine immunization, and auxiliary antibiotic and antiviral drug treatment. However, the disadvantage is that manual vaccination is labor-intensive and poultry have a large...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61P31/14A61P31/16A61K39/17A61K39/155
Inventor 吴润生包松英林拱阳
Owner 兆丰华生物科技(南京)有限公司
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