Saprolegnia Evans Blue intravital staining method

A technology of Evans blue and in vivo dyeing, applied in the field of Evans blue in vivo dyeing, can solve the problems of missed prevention and control timing, long time for culture test, labor and time-consuming, etc., and achieve the effect of short time.

Inactive Publication Date: 2015-10-21
HANGZHOU ENJOY ENVIRONMENTAL PROTECTION TECH
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  • Application Information

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Problems solved by technology

Due to the long time of culturing and testing, it is labor-intensive and time-consuming, so the best timing of prevention and control is often missed, which brings economic losses ...

Method used

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Embodiment 1

[0044] HGSM01 Saprolegnia Strain: It was isolated from golden crucian carp oviparous Saprolegniasis in Chuzhou City, Anhui in April 2013. In October 2013, the biological samples were sent to Shanghai Ocean University (National Aquatic Animal and Plant Pathogen Bank) for molecular biological identification of the strain. Confirmed as saprolegniasis.

[0045] Take the rapeseed bait (covered with Saprolegnia mycelia) and the just released Saprolegnia spores (refer to the Saprolegniasis purification operation) grown in the Saprolegnia semi-solid plate, and divide them into 2 groups, 6 in each group in parallel. One group was bathed in 80°C water bath for 10 minutes (test group), and the other group was not treated (reference group). Three samples were taken in parallel in each group, then dipped in 2‰ Evans blue aqueous solution for 2 minutes, and microscopic examination was performed. Spores) are all dyed light blue, and the reference group is colorless; the saprolegnia materials...

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Abstract

The invention discloses a saprolegnia Evans Blue intravital staining method. The method comprises the following steps of separating Saprolegnia strains, purifying and culturing the Saprolegnia strains, obtaining Saprolegnia spores, preparing Evans Blue dye liquor, dyeing and the like. The activity of water mildew mycelia can be quickly judged by detecting whether the dyed water mildew mycelia and the spores become blue or not under a microscope; and the percentage of living cells and dead cells can be simply calculated according to the percentage of colored cells in total cells, so as to rapidly judge the vitality of Saprolegnia mycelia. The method has the characteristics of simplicity, feasibility, intuition and the like, and is suitable for scientific research institutions, schools, technique popularization stations and aquaculture companies.

Description

technical field [0001] The invention belongs to the technical field of aquaculture disease prevention and control, and in particular relates to an Evans blue living body dyeing method for aquatic animal parasitic Saprolegnia. The invention is simple and easy to implement, and is suitable for application in scientific research institutes, schools, technology extension stations and aquaculture companies. technical background [0002] Saprolegniasis is a kind of saprolegniasis parasitic disease on the surface of aquatic animals (such as: fish, shrimp, crab, turtle, frog, etc.). can be infected. When the surface skin of aquatic animals is damaged due to physical and chemical factors, or bacterial or viral infection, the zoospores of saprolegniasis invade the wound, absorb the nutrients in the skin and its tissues and grow rapidly, and the hyphae entwine and stick to the tissue cells of the wound. Attachment, tissue inflammation, necrosis, aquatic animals due to heavy burden, s...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12R1/645
Inventor 周呈祥叶伟武同现鹏张繁荣仲崇艳凌武海夏文伟
Owner HANGZHOU ENJOY ENVIRONMENTAL PROTECTION TECH
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