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Laminin membrane for assisting iPS-RPE transplantation

A laminin membrane, laminin technology, applied in the field of protein membranes, can solve the problems of inability to properly integrate surrounding tissues, stem cell death, heterogeneity, etc., to improve cell survival, reduce pain, and repair diseased tissue. Effect

Active Publication Date: 2015-11-04
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This is because the new environment is full of complex unknowns, and transplanted stem cells often die or fail to integrate properly into surrounding tissues
Recent studies have shown that hydrogel can promote the therapeutic effect of stem cells, but its main components are hyaluronic acid and methylcellulose, and there are still problems of heterogeneity and degradation

Method used

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  • Laminin membrane for assisting iPS-RPE transplantation

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Embodiment 1

[0022] A kind of embodiment of the preparation method of laminin described in the present invention, described preparation method is as follows:

[0023] Take blood from the heart of isogenic rats, place it for 10 minutes, centrifuge at 1000rpm for 20 minutes, discard the precipitate, keep the upper layer of serum, remove chylomicrons, and separate serum laminin; purify serum laminin by gel chromatography, and use DEAE cellulose anion exchange chromatography Serum laminin is purified and concentrated to obtain said laminin. Transfer the concentrated serum laminin into a Petri dish and place it in a refrigerator at 4°C until the air bubbles are removed.

[0024] An embodiment of the preparation method of the laminin film for assisting iPS-RPE transplantation described in the present invention, the preparation method is as follows:

[0025] Add glutaraldehyde to the laminin solution prepared above, the concentration of glutaraldehyde in the solution is 5 mg / mL, and then put it ...

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Abstract

The invention provides a laminin membrane for assisting iPS-RPE transplantation and an iPS-RPE cell patch prepared from the membrane. The laminin membrane can promote migration, differentiation and regeneration of injured and degenerated retina cell on a provided collagen scaffold to form the same tissues of the retina cell and simultaneously can interfere with non-sequential fibroblast proliferation so as to avoid scar formation. According to the invention, mechanical properties of an autoserum laminin biomaterial can be enhanced, subretinal transplantation can be realized, defects of poor mechanical property, high immunogenicity, difficulty in degradation and low survival rate after stem cell transplantation in the prior art are overcome, diseased tissue is effectively repaired, and a new treatment method and thinking is provided to minimize pains of patients.

Description

technical field [0001] The invention relates to a protein film, in particular to a laminin film for assisting iPS-RPE transplantation. Background technique [0002] Refractory retinopathy is a kind of retinal tissue damage or degenerative disease, including age-related macular degeneration, Stargardt disease and retinitis pigmentosa, etc., the prevalence rate of people over 50 years old in my country is more than 20%, and it has become an increasingly prominent problem in my country. The main blinding eye disease, its common pathological feature is the degeneration and death of retinal pigment epithelial cells (RPE cells), leading to blindness. The development of stem cell technology has made it possible for new RPE cells to replace missing or degenerated RPE cells. [0003] However, after cell transplantation, even if immunosuppressants are applied, the transplanted cells cannot survive for a long time, and the clinical efficacy is not significant, so it is still not widely...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/50
Inventor 刘奕志郑颖丰
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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