37 results about "Retina cell" patented technology

Disclosed are immortalized human embryonic retina cells, having a nucleic acid sequence encoding an adenoviral E1A protein integrated into the genome of the cells, and further comprising a nucleic acid sequence encoding an enzyme involved in post-translational modification of proteins, such as a sialyltransferase, wherein said nucleic acid sequence encoding the enzyme involved in post-translational modification of proteins is under control of a heterologous promoter. Methods for producing recombinant proteins from such cells and obtaining such recombinant proteins having increased sialylation are provided as are novel compositions of isoforms of erythropoietin .

A retina implant including a chip adapted to be implanted into the interior of eye in subretinal contact with the retina. The chip has a plurality of pixel elements on a side thereof facing the lens for receiving an image projected into the retina and a plurality of electrodes for stimulating retina cells. The implants further includes a receiver coil for inductively coupling thereinto electromagnetic energy. The receiver coil coupled to a means for converting an alternating voltage induced into the receiver coil in a direct voltage suited for supplying the chip. The receiver coil is configured as a component separate from the chip, and for being positioned on the eye ball outside the sclera. The chip is connected to the receiver coil via a connecting lead which, in the implanted condition interconnects the interior and the exterior of the eye ball.

Disclosed are immortalized human embryonic retina cells, having a nucleic acid sequence encoding an adenoviral E1A protein integrated into the genome of the cells, and further comprising a nucleic acid sequence encoding an enzyme involved in post-translational modification of proteins, such as a sialyltransferase, wherein said nucleic acid sequence encoding the enzyme involved in post-translational modification of proteins is under control of a heterologous promoter. Methods for producing recombinant proteins from such cells and obtaining such recombinant proteins having increased sialylation are provided as are novel compositions of isoforms of erythropoietin.

Methods and apparatuses for configuring an implantable device to interface with retina cells are described. The device may comprise an array of pixel units capable of stimulating the retina cells are described. The pixel units may operate in a mode of operation selected from a plurality of modes including a normal mode and a calibration mode. A control circuitry of the device may be configured to switch the mode of operation for the pixel units. In one embodiment, the pixel units may be configured to receive light for stimulating the retina cells during the normal mode to enable perception of the light. During the calibration mode, the pixel units may be configured to adjust amount of stimulation to the retina cells.

The invention discloses a process for recombinant production of blood coagulation Factor VIII in an immortalized human embryonic retina cell, said cell expressing at least an adenoviral E1A protein and comprising a nucleic acid sequence encoding said Factor VIII, said nucleic acid sequence being under control of a heterologous promoter, said process comprising culturing said cell and expressing the Factor VIII in said cell, and harvesting the expressed Factor VIII. Cells that can be used in the process of the invention are also provided.

A method of analyzing an OCT image. An OCT image has many differently colored dots. The system detects the number of differently colored dots, quantifies them and performs statistical analyses to determine a likelihood of disease. The different colored dots correspond to different retina cell types and structures.

The invention provides an immortalized human retina cell expressing E1A and E1B proteins of an adenovirus, wherein the cell has recombinant nucleic acid encoding an IgA molecule in expressible format. Also provided is a method for recombinant production of an IgA molecule, the method involving culturing a cell of the invention and expressing the recombinant nucleic acid encoding an IgA.

The invention discloses a traditional Chinese medicine for treating amblyopia eye diseases and relates to a preparation prepared from the traditional Chinese medicines as raw materials. The traditional Chinese medicine is prepared from semen cassiae, radix paeoniae alba, salvia miltiorrhiza, medlar, tuckahoe, dendrobe, kudzuvine root, tuber of dwarf lilyturf, tribulus terrestris, adenophora tetraphylla, yam, forsythia core, motherwort fruit, lophatherum gracile, antelope horn powder, glabrousleaf pittosporum seed and fructus psoraleae. The clinical use result of the traditional Chinese medicine shows that the prescription has the functions of enhancing the capability of supplying blood to caridiac muscle, promoting the blood circulation and improving the development process of eye parts of patients and promoting the repair of optic nerve and retina cells as well as the efficacies of nourishing the kidney, nourishing the liver and improving acuity of vision; the prescription can be used for treating amblyopia, hyperopia, yellow spot and other diseases caused by various reasons; and the traditional Chinese medicine has unique formula and curative effect, especially has remarkable effects for infants and juvenile patients and can ensure that the symptoms of the patients are eliminated and the visions of the patients are recovered.

This invention relates to the expression method of adeno-associated virus-mediated gene in retinal cells. This invention can significantly improve the transfer efficiency and gene expression level of adeno-associated virus-mediated gene in retinal cells by using a combination of different types of adeno-associated virus, and has no toxic or side effect. Experimental results show that at a certain dosage range, replication-competent adenovirus and replication-defective adenovirus can significantly accelerate AAV2-mediated gene expression, and no cell lesion is observed. This invention can be used for gene treatment of ocular fundus diseases, such as retinosis, maculopathy, diabetic retinopathy, uveitis, choroidal neovascularization, optic nerve degeneration and damage.

The present invention discloses a retina cell microscopic imaging system which can conduct stratified scanning and comprises an illuminating system and a microscopic imaging optical unit. The inside of an optical path between the illuminating system and a human eye (601) is provided with a stratified scanning optical unit which comprises a first object lens (501) and a second object lens (502). The first object lens (501) and / or the second object lens (502) can move critically along the axial direction. The parallel incident illumination beams of the illuminating system are focused on certain film inside a human eye retina (603) through the second object lens (502) and the first object lens (501) sequentially. During the stratified scanning, a focusing face formed on the retina (603) moves correspondingly at each cell layer inside the retina (603) along with the movement of the first object lens (501) and the second object lens (502). A small depth of focus imaging optical unit is used as the microscopic imaging optical unit. The retina cell microscopic imaging system of the present invention has simple structure and can effectively conduct stratified observation towards each film forming the retina.

The invention relates to peanut blend oil containing docosahexaenoic acid (DHA). The peanut blend oil is characterized by comprising the following components by weight percent: 1.37-2.74% of DHA algal oil, 71% of peanut oil, 18.96-20.33% of palm oil and 7.3% of flaxseed oil. The invention provides the peanut blend oil containing the DHA, and a preparation method of the peanut blend oil. The peanut blend oil not only is rich in DHA, but also is rich in oleic acid, linoleic acid, palmitic acid and linolenic acid; the composition of all fatty acids is balanced in nutrition and reasonable, and the demand of people for the DHA can be basically met; furthermore, the peanut blend oil is capable of providing enough saturated fatty acid and monounsaturated fatty acid, thus promoting the growth and development of brain cells, retina cells, nerve cells and the like, delaying senescence, controlling cardiovascular and cerebrovascular diseases, immune function decline and senile dementia, and the like; the peanut blend oil is lower in price; when the peanut blend oil is used for daily diet of common people, the DHA needed by the people can be ingested; and furthermore, the peanut blend oil is good in stability, longer in shelf life and easy in industrialization.

The invention discloses a blended blueberry juice and a preparation method of the blended blueberry juice. The blended blueberry juice is prepared from the following raw materials in parts by weight: 30 to 40 parts of highbush blueberries, 20 to 25 parts of juicy peaches, 1 to 6 parts of pectin, 12 to 15 parts of black currant, 6 to 8 parts of granulated sugar, 1 to 3 parts of pectinase, 1 to 4 parts of cellulase, 2 to 4 parts of hemicellulase, 1 to 3 parts of protease, 12 to 15 parts of lonicera edulis, 22 to 24 parts of grape seeds, 1 to 4 parts of propolis and 3 to 5 parts of vitamin E. According to the blended blueberry juice disclosed by the invention, a large amount of anthocyanin active ingredients in blueberries are reserved, and the blended blueberry juice contains rich nutritional ingredients and has the functions of activating retinas, promoting the regeneration of rhodopsin in retina cells, preventing myopia, increasing the eyesight and removing eye fatigue.

The invention discloses a composition capable of promoting the development of eyes and brains of children and alleviating asthenopia and a preparation method of the composition. The composition is prepared from the following components in parts by weight: lutein ester, xanthophyll, blueberry powder, vitamin A, taurine, vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B12, vitamin C, calcium pantothenate and zinc gluconate. The composition can be made into many categories of foods, preferably beverages, dairies, biscuits, candy products, and special meals. According to the composition disclosed by the invention, raw materials are scientific in compatibility, so that nutrients required for the eyes and the brains are complemented, the development of retina cells and nerve cells of the children is promoted, and the composition can also alleviate the asthenopia.

The invention discloses a method for synthesizing human eye vision system perception effect in a dark environment, which provides an intuitive control technology of human vision system perception effect in a night environment, in particular a dark environment for users. By the method, a user can designate an arbitrary target lighting environment by inputting lighting intensity and frequency spectrum, and the human eye vision system perception effect of a scene shown in a source image in the target lighting environment can be automatically synthesized. In the method, color frequency spectrum is reestablished according to the source image, and response of the retina cell under the source lighting environment is calculated; the adapting situation of the retina cell in the target light environment is simulated according to measurement data, and response of the adapted retina cell is calculated; and finally, the image is non-uniformly blurred by using a two-sided filter to make an acutance loss effect in the target lighting environment. Application of the method is not used in a night environment only, also can be used for synthesizing the human eye vision system perception effect in other lighting environments.

The invention discloses a composition for promoting the development of eyes and brains of babies and a preparation method thereof. The composition is prepared from xanthophyll ester, xanthophyll, blueberry fruit powder, vitamin A, taurine, vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B12 and zinc gluconate according to certain weight parts. The composition can be prepared into a plurality of food categories, preferably beverages, milk products, biscuits, candies and special diets. The composition disclosed in the invention is prepared by scientifically matching the raw materials, can supplement nutrients needed by the eyes and brains, and promote the development of retina cells and neurons of the babies.

The embodiment of the invention discloses a composition with the function of relieving visual fatigue. The composition is composed of, by weight, 10,000-20,000 parts of blueberries, 500-1,000 parts ofChinese wolfberry fruits, 1-1.8 parts of nicotinamide and 1-1.8 parts of xanthophyll. The composition can be used for preparing health-care foods or medicine for relieving visual fatigue, under the synergistic effect of the blueberries, the Chinese wolfberry fruits, nicotinamide and xanthophyll, the SOD and GSH-Px activity of retina cells can be effectively improved, the content of total proteinsof the retina cells can be effectively increased, the MDA content is remarkably decreased, the completeness of all layers of cells of the retina can be further protected, damaged cells are improved,the thickness of the outer nuclear layer of the retina is increased, and therefore a good effect of relieving visual fatigue is realized. In addition, the composition is free of toxic and side effectsand high in safety.

The invention discloses induced pluripotent stem cells of RPE65 gene mutation patients. The obtained induced pluripotent stem cells of RPE65 gene mutation patients are in non-gene integration, so thatthe subsequent application safety can be improved. The obtained RPE65-hiPSCs contains 2 new gene site mutation (c.200T>G(p.L67R) and c.430T> C ( p.Y144H ) ), and a biomaterial is provided for nosogenesis research of such diseases. The obtained RPE65-hiPSCs has polyembryony layer differentiation potency (nerve, cartilage, mucosa and the like), comprises the capacity for differentiation for all retina cells, can provide seed cells for homogeneous or self cell transplantation, and provides a cell material for downstream research.

The present invention discloses a retina cell microscopic imaging system with a stray light eliminating optical system, which comprises an illuminating system and a microscopic imaging optical system. The inside of an optical path between the illuminating system and a human eye (601) is provided with a first polarization beam splitter prism PBS (401) which eliminates the stray light, and the first polarization beam splitter prism PBS (401) is also arranged inside the optical path between the human eye (601) and the microscopic imaging optical system. The retina cell microscopic imaging system of the present invention provides a retina cell microscopic imaging system with the stray light eliminating optical system, which uses a new stray light eliminating technology, has simple structure and can effectively improve the retina imaging definition.

The invention provides a laminin membrane for assisting iPS-RPE transplantation and an iPS-RPE cell patch prepared from the membrane. The laminin membrane can promote migration, differentiation and regeneration of injured and degenerated retina cell on a provided collagen scaffold to form the same tissues of the retina cell and simultaneously can interfere with non-sequential fibroblast proliferation so as to avoid scar formation. According to the invention, mechanical properties of an autoserum laminin biomaterial can be enhanced, subretinal transplantation can be realized, defects of poor mechanical property, high immunogenicity, difficulty in degradation and low survival rate after stem cell transplantation in the prior art are overcome, diseased tissue is effectively repaired, and a new treatment method and thinking is provided to minimize pains of patients.

The present invention discloses a retina cell microscopic imaging system which uses a shearing interference phase contrast technology. An imaging optical system is provided with an interference phase contrast optical unit which converts phase type transparent retina structure into a corresponding image with light intensity change. The retina cell microscopic imaging system comprises two pieces of holophotes (1302 and 1304), two pieces of half-reflecting and half-transmitting mirrors (1301 and 1302), a compensating plate (1305) and an optical wedge set (1306). Two pieces of the holophotes (1302 and 1304) and two pieces of the half-reflecting and half-transmitting mirrors (1301 and 1302) are arranged at the positions of four peaks shaped as rectangle. The compensating plate (1305) is arranged between the half-reflecting and half-transmitting mirror (1301) and the holophote (1304). The optical wedge set (1306) is arranged between the holophote (1302) and a half-reflecting and half-transmitting mirror (1303). The retina cell microscopic imaging system of the present invention can clearly reflect the structural detail on the retina, and a formed retina image has third dimension and can change interference color; illumination aperture is not limited; the periphery is not provided with false band; the position of the interference phase contrast optical unit can be arranged flexibly.

The invention constructs a human embryo retina transformed cell line SL-2 by transforming adenovirus gene fragments into human embryo retina cells. The cell line can be used for producing biological products such as adenovirus and the like, and has obvious advantages. The cell line can also be used for producing influenza virus, herpes simplex virus, measles virus, rotavirus and other viruses, polypeptides and other biological products.

The invention discloses sea-buckthorn black fruit mountain ash berry eye-protection milk tablets and a preparing method thereof. The sea-buckthorn black fruit mountain ash berry eye-protection milk tablets comprise dry powder of a sea-buckthorn extract, dry powder of a black fruit mountain ash berry extract, defatted milk, citric acid and xylitol, and the eye-protection milk tablets are prepared through a series of steps. The prepared sea-buckthorn black fruit mountain ash berry eye-protection milk tablets are convenient to carry and eat and have a strong eye protection function, the harm of blue light to the eyes can be effectively avoided, regeneration of rhodopsin in retina cells can be promoted, severe myopia and retinal detachment can be prevented, and the vision can be enhanced and protected; meanwhile, cell and organ damage of free radicals in the human body can be resisted, and cardiovascular sclerosis, coronary heart diseases, phymatosis and other symptoms triggered by the body are effectively prevented.

The invention discloses a concentrated blueberry juice and a preparation method thereof. The concentrated blueberry juice is prepared from the following raw materials in parts by weight: 50 to 55 parts of blueberries, 4 to 8 parts of pectinase, 7 to 12 parts of grape skin, 17 to 20 parts of tremella fuciformis, 4 to 6 parts of shell powder, 15 to 17 parts of cranberries, 5 to 7 parts of black rice, 1 to 3 parts of tartaric acid, 3 to 7 parts of coenzyme, 11 to 13 parts of atap seeds, 1 to 3 parts of edible salt, 11 to 13 parts of edible alcohol and 1 to 3 parts of sodium citrate. The concentrated blueberry juice disclosed by the invention has a good taste, reserves a large amount of anthocyanin active ingredients in the blueberries and has the functions of activating retinas, promoting the regeneration of rhodopsin in retina cells, preventing myopia, increasing the eyesight and removing eye fatigue.

The invention discloses a retina cell scaffold biological surgical binder and a preparing method thereof. The retina cell scaffold biological surgical binder comprises laminin, type-IV collagen, nidogen, human heparin sulphate protoglycans, fibroblast growth factors, matrix metalloproteinase and phosphate buffer salt solution. By the adoption of the retina cell scaffold biological surgical binder, degradable human iPSCs-derived retina nerve scaffold (iRMP) can be bound with a host retina nerve layer, and effective integration of a cell biological scaffold and a host retina is guaranteed. The tissue engineering technology and clinical practice are combined, and the retina cell scaffold biological surgical binder has certain theoretical and practical application value for regenerative medicine fundamental research and ophthalmology clinical treatment, promotes optic nerve system damage repair and nervous tissue engineering research, and lays a theoretical and experimental foundation for tissue engineering transplantation therapy of visual damage.

The invention discloses a DHA-containing olive blending oil which comprises 97.26-98.63 percent (weight) of olive oil and 1.37-2.74 percent (weight) of DHA algae oil. The invention further provides a preparation method of the DHA-containing olive blending oil. The DHA-containing olive blending oil disclosed by the invention contains DHA, and further contains oleic acid and palmitic acid, is capable of meeting the demand of the public for the DHA, also provides sufficient saturated fatty acid and monounsaturated fatty acid, is capable of promoting growth and development of brain cells, retina cells, nerve cells and the like, delaying the senescence and preventing cardiovascular and cerebrovascular diseases, immunologic function decline, senile dementia and the like; compared with a conventional DHA product, the olive blending oil is relatively low in price so as to be affordable for the public; the public can intake the DHA of a required amount in daily diets, thus the DHA-containing olive blending oil is very convenient; the DHA-containing olive blending oil has good stability and relatively long shelf life, and is easy to industrialize.

The invention belongs to the field of pharmacy, and relates to a medicinal composition for protecting optic nerve of glaucoma and a preparation method thereof. The medicinal composition is prepared from 0.1 to 10 ug of sodium valproate and a therapeutic gene-carried adeno-associated virus carrier of 1*10<e9>-1*10<e12> virus particles. A compound preparation consisting of the sodium valproate and the therapeutic gene-carried adeno-associated virus carrier is prepared, or the sodium valproate and the therapeutic gene-carried adeno-associated virus carrier are respectively prepared into a medicinal preparation and the two separate medicinal preparations are packaged into the same medicine box. The medicinal composition can overcome defects of the conventional nerve protection therapy, can protect retinal ganglion cells (RGCs) through multiple links, also can drive therapeutic genes carried by the adeno-associated virus carrier to be efficiently expressed for a long time in retina cells to achieve a synergic effect on neuroprotective therapy for glaucoma, and can effectively improve the effect of the neuroprotective therapy for the glaucoma.

The invention discloses a measuring system and method for laser image speckles based on human eye features. The measuring system comprises a lens, a photosensitive element and a data processor, wherein the lens is used for imaging a laser image onto the photosensitive element, the photosensitive element is used for imaging the laser image obtained by the lens, and the data processor is used for obtaining data for representing speckle characteristics of the laser image according to imaging image data. The effective aperture of the lens is the size of the human eye pupil, the focal length of the lens is the focal length of the human eye, the pixel size of the photosensitive element approximates the size of the human eye retina cells, and a light intensity response curve of the photosensitive element is approximately matched with the light intensity sensing curve of the human eye. According to the measuring system and method, measurement of the laser image speckles is more accurate, and no equipment cost needs to be added.