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Aerobic denitrifying bacterium strain and application thereof

A technology of aerobic denitrifying bacteria and strains, applied in the fields of bioengineering and environmental engineering, can solve the problems of limited practical application research and single strains, and achieve high aerobic denitrification performance

Active Publication Date: 2015-11-11
黑龙江佰瑞德环境生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] So far, aerobic denitrification strains are mainly concentrated in the genus Pseudomonas, Bacillus and Aeromonas. The strains are relatively single, and the research is mostly focused on the degradation gene and degradation mechanism, and the practical application research is limited.

Method used

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  • Aerobic denitrifying bacterium strain and application thereof
  • Aerobic denitrifying bacterium strain and application thereof
  • Aerobic denitrifying bacterium strain and application thereof

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specific Embodiment approach 1

[0018] Specific embodiment 1: An aerobic denitrifying bacterium of the present embodiment, the bacterial strain is (Aeromonassp.) AD-3, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation address is Beichen West, Chaoyang District, Beijing No. 3, Courtyard No. 1, the preservation date is July 14, 2015, and the preservation number is CGMCCNo.11069.

[0019] The (Aeromonassp.) AD-3 of the present embodiment is obtained by the following methods:

[0020] a. Take the activated sludge at the end of the anoxic section of the SBR reactor running stably for half a month as the separation mud;

[0021] b. Separation using the dilution mixed plate method: the strains that have been isolated are placed in a denitrification medium under aerobic conditions for denitrification test, and at the same time, nitrate reduction gas production test and heterochromatic particle staining (methylene blue) are as...

specific Embodiment approach 2

[0046] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the bacterial strain is isolated from activated sludge. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0047] Embodiment 3: This embodiment is an application of aerobic denitrifying bacteria, which is used for denitrification performance in nitrogen-containing wastewater treatment.

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Abstract

The invention relates to an aerobic denitrifying bacterium strain and application thereof. The aerobic denitrifying bacterium strain is Aeromonas sp. AD-3, and is collected by China General Microbiological Culture Collection Center. The collection address is Beichenxi Road No.1 Yard 3#, Chaoyang District, Beijing City; the collection date is July 14th, 2015; and the collection number is CGMCC No.11069. The Aeromonas sp. AD-3 is used for treating nitrogenous wastewater. The gene of the Aeromonas sp. AD-3 has higher aerobic denitrifying property. The total nitrogen removal rate is up to 87.7%, and thus, the Aeromonas sp. AD-3 has significant meanings in the field of water treatment due to the characteristics.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering, relates to an aerobic denitrification bacterial strain and its application, and simultaneously provides the denitrification efficiency of the bacterial strain. Background technique [0002] At present, with the rapid development of the national economy and the continuous deepening of the urbanization process, the total discharge of urban domestic and industrial wastewater is also increasing year by year. The widespread use of pesticides, chemical fertilizers and synthetic detergents has increased the concentration of nutrients in water bodies, and nitrogen is one of the main causes of eutrophication in water bodies. Conventional biochemical treatment process can effectively reduce BOD and SS in sewage, but when there are N, P and other nutrients in sewage, it can only remove 30-40% of nitrogen in sewage, and a large amount of nitrogen-containing sewag...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01
CPCY02W10/10
Inventor 马放朱宏源皮珊珊杨基先李昂孙移鹿张雪宁
Owner 黑龙江佰瑞德环境生物科技有限公司
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