30 results about "Aeromonas sp" patented technology

The invention belongs to the technical field of biological enzymes, and in particular relates to a pullulanase, a pullulanase producing strain and application of the pullulanase. The pullulanase is derived from Aeromonas sp. As in bacteria. The pullulanase produced by the strain is pullulanase type I, and can specifically hydrolyze alpha-1,6 glycosidic bond in pullulan or starch. The pullulanase is expressed under an induction condition in which 1.5% soluble starch is used as a substrate. Under a condition of medium pH of 7.5 and culture temperature of 30 DEG C, shake-flask fermentation is carried out for 48h to produce the pullulanase at a level up to 24.8U / mL. For the pullulanase produced by the strain, the optimum reaction temperature is 60 DEG C, the optimum pH is 6.0, and the relative enzyme activity in the pH range of 6.0-9.0 is greater than 90 percent. After treatment for 50min at 60 DEG C, the residual activity of the pullulanase reaches about 50 percent. The pullulanase type I provided by the invention can be applied to industries such as starch processing, environment protection, foods, medical care and the like.

The invention provides a simple method for preparing high-purity L-amino acid by biocatalysis. A viable cell of bacteria Aeromonas sp. (preserving number CGMCC 2226) containing a plurality of enzymes can selectively oxidize D-type isomer in racemate amino acid under the condition of aerating air to leave unreacted high-purity L-amino acid. The method is reasonable in design without common derivative steps of protection in advance and protection once again in the prior method, is capable of directly and selectively transforming the D-type amino acid in the racemate, does not use metal catalysts, organic solvent or accessory ingredients during preparing process, and carries out reactions in neutral condition without chemical discharge except reproducible biomass, thereby belonging to green and environment-friendly production process. The method has the advantages of simple and short route, high efficiency, resource conservation and simple steps. The method is applicable to elimination of D-type antipode in more amino acids in different types and various mixtures possibly formed by the amino acids.

The invention relates to an aerobic denitrifying bacterium strain and application thereof. The aerobic denitrifying bacterium strain is Aeromonas sp. AD-3, and is collected by China General Microbiological Culture Collection Center. The collection address is Beichenxi Road No.1 Yard 3#, Chaoyang District, Beijing City; the collection date is July 14th, 2015; and the collection number is CGMCC No.11069. The Aeromonas sp. AD-3 is used for treating nitrogenous wastewater. The gene of the Aeromonas sp. AD-3 has higher aerobic denitrifying property. The total nitrogen removal rate is up to 87.7%, and thus, the Aeromonas sp. AD-3 has significant meanings in the field of water treatment due to the characteristics.

The invention discloses a method for efficiently degrading azo dye in dye wastewater by using bacteria. The method comprises the following specific steps: (1) inoculating a strain Aeromonas sp.DH-6 into an LB (Luria-Bertani) culture medium, culturing over night in a shaker in the rotation speed of 150-200rmp at 25-35 DEG C, so as to obtain a great amount of bacteria; (2) inoculating the over-night culture which is 2-8% in inoculation amount into the dye wastewater containing azo dye, and standing to culture and decolor, wherein the decoloring pH value is 3.0-7.0, the temperature is 5-45 DEG C, and the decoloring culture time is more than 4 hours. The method disclosed by the invention is still high in decoloring rate for methyl orange under acid conditions at relatively high temperature (about 40 DEG C). Besides, no remarkable influence is caused by most meal ions in coloring, the decoloring rate can be still approximate to 90% when 1000mg / L of the dye is cultured for 48 hours by using the bacteria, and thus the decoloring effect is relatively good. Therefore, compared with a conventional method, the bacterium DH-6 can be used for more efficiently degrading the azo dye with low energy consumption, and is higher in environment applicability, free of secondary pollution to the environment, and wider in research value and application prospect.

The invention provides aeromonas (Aeromonas sp.) 6-38. The aeromonas is separated from frozen livestock and poultry sewage and has a collection number of CGMCC No. 4402. The strain can be used for effectively decomposing starch, protein and fat simultaneously, is prepared into a microbial agent under a low-temperature environment and is used in livestock and poultry excrement compost, so that temperature rise of the compost can be promoted; and the microbial agent has a simple preparation process and a short fermentation period and is easy to store.