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Composite bacteria preparation used for disinfection, and preparation and application method thereof

A technology of microorganisms and microbial flora, applied in the directions of bacteria, fungi, sterilization/microdynamic water/sewage treatment, etc. Disorderly development of varieties and other problems, to achieve the effect of not producing drug resistance of pathogenic bacteria, restoring the micro-ecological balance of water bodies, and leaving no drug residues

Inactive Publication Date: 2017-06-16
SHANGHAI HONGBAO GREEN AQUATIC PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] my country's aquaculture industry has made great progress since the 1980s. In terms of industrial characteristics, it shows that there are many varieties of aquaculture and high economic value. However, at the same time, there have also been phenomena such as the disorderly development of a single species, blindly increasing the breeding density, etc., which are not conducive to the healthy and sustainable development of the breeding industry, resulting in deterioration of water quality and frequent occurrence of breeding diseases

Method used

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  • Composite bacteria preparation used for disinfection, and preparation and application method thereof
  • Composite bacteria preparation used for disinfection, and preparation and application method thereof
  • Composite bacteria preparation used for disinfection, and preparation and application method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, composite disinfection microorganism 1# is prepared from the component of following mass percentage content:

[0037]

[0038] The preparation method of composite disinfection microorganism 1#:

[0039] Step 1. Stir the bacteriophage Bdellovibrio, Bacillus, microbial nutrient solution and physiological saline evenly;

[0040] Step 2. Adsorb the above-mentioned liquid mixture on diatomaceous earth (liquid-solid mass ratio 1:1);

[0041] Step 3, drying at a temperature of 15-35° C. to make the water content lower than 30%.

Embodiment 2

[0042] Embodiment two, composite disinfection microorganism 2# is prepared from the component of following mass percentage content:

[0043]

[0044] The preparation method of composite disinfection microorganism 2#:

[0045] Step 1. Stir the Bdellovibrio phage, lactic acid bacteria, yeast, microbial nutrient solution mixture, and bacterial physiological saline evenly;

[0046] Step 2, the above-mentioned liquid mixture is adsorbed on the zeolite powder (the mass ratio of liquid to solid is 1:0.5);

[0047] Step 3: freeze-drying at a temperature of -15-0° C. to make the water content lower than 10%.

Embodiment 3

[0048] Embodiment three, composite disinfection microorganism 3# is prepared from the component of following mass percentage content:

[0049] Bdellovibrio bacteriophage 5

[0050] Phage Bdellovibrio Nutrient Solution 1.5

[0051] Bacterial saline 92.5

[0052] The preparation method of composite disinfection microorganism 3#:

[0053] Step 1, stirring the Bdellovibrio phage, Bdellovibrio phage nutrient solution, and bacterial physiological saline evenly;

[0054] Step 2. Adsorb the above-mentioned liquid mixture on the zeolite powder (liquid-solid mass ratio 1:1);

[0055] Step 3, drying at a temperature of 15-35°C to make the water content 10-30%.

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PUM

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Abstract

The invention provides a composite bacteria preparation used for disinfection. The composite bacteria preparation is composed of, by mass, 1 to 25% of a microbial community, 0.5 to 10% of a microbial nutrient solution, and 65 to 95% of bacteria normal saline. The microbial community with bacteria killing effect is taken as a main microbial community, the content of effective probiotics is capable of reaching 3*10<7> cfu / g; the main microbial community possesses killing, phagocytosis, and rapid lysis effect on pathological bacteria such as vibrio parahaemolyticus, aeromonas hydrophila, aeromonas punctata, escherichia coli, salmonella, and edwardsiella spp, so that the composite bacteria preparation is capable of reducing the content of pathological bacteria in water bodies obviously, killing bacteria with bacteria, and repairing water body microecological balance.

Description

technical field [0001] The invention relates to the field of aquatic organism cultivation, in particular to composite disinfecting microorganisms and methods for preparing and using the same. Background technique [0002] my country's aquaculture industry has made great progress since the 1980s. In terms of industrial characteristics, it shows that there are many varieties of aquaculture and high economic value. However, at the same time, the disorderly development of a single species and blindly increasing the breeding density are not conducive to the healthy and sustainable development of the breeding industry, resulting in deterioration of water quality and frequent occurrence of breeding diseases. [0003] For example: pathogenic strains can infect fish, amphibians, reptiles, birds, mammals and other animals, clinically characterized by acute hemorrhagic sepsis. Chronic infection is mainly manifested as skin ulcers or enteritis. People can also suffer from diarrhea and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F1/50
Inventor 邹国忠
Owner SHANGHAI HONGBAO GREEN AQUATIC PROD CO LTD
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