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Method for preparing alga oligosaccharide with anticoagulation activity

A technology of anticoagulant activity and seaweed oligosaccharide is applied in the field of preparing seaweed oligosaccharide with anticoagulant activity, and can solve the problems of high preparation cost, toxicity and the like

Inactive Publication Date: 2016-03-30
潘崇良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies in this field have shown that after seaweed polysaccharides are hydrolyzed by enzymes, chemical methods are generally required to increase the content of sulfate groups to improve anticoagulant activity. However, chemical methods (such as adding chlorosulfonic acid-dimethylformamide Reagents) To increase the content of sulfuric acid groups, chemical reagents must be used. These reagents are mostly toxic and require further removal and purification, and the preparation cost is relatively high

Method used

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  • Method for preparing alga oligosaccharide with anticoagulation activity
  • Method for preparing alga oligosaccharide with anticoagulation activity
  • Method for preparing alga oligosaccharide with anticoagulation activity

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1: the powder preparation and heat treatment of Sargassum, Geliflower, Qinghai Cai

[0060] In this experiment, Sargassum siliquosum, Gelidium amansii, and Monostromanitidum were respectively selected as the seaweed sources of Sargassum, Gelidium amansii, and Qinghai vegetable of the present invention, and the above-mentioned seaweed was heat-treated to extract polysaccharides , to obtain seaweed polysaccharide liquid from Sargassum, Gemweed, and Qinghai. In short, Sargassum, Gemweed, and Qinghai Cai were washed with running water, dried, and then ground to obtain powders of Sargassum, Gemweed, and Qinghai Cai respectively. Take 5g of Sargassum, Glycyrrhizae, and Qinghaicai powders and add them into 100mL of water respectively, and heat-extract them in a sterilized kettle at 121°C for 10 to 30 minutes to obtain seaweed polysaccharide liquids from Sargassum, Glycerus, and Qinghaicai, respectively.

Embodiment 2

[0061] Example 2: Inducing marine strains to produce saccharolytic enzymes with polysaccharides from Sargassum, Geliflower, and Qinghai Vegetables

[0062] In this experiment, the marine strains were respectively cultured in the bacterial culture solution containing polysaccharides from Sargassum, Geliflower, and Qinghai vegetables, and induced to produce saccharolytic enzymes to obtain crude enzyme solutions. In short, the seaweed polysaccharide solution from Sargassum, Glycyrrhizae, and Qinghai vegetable prepared in Example 1 was added to the marine bacterial culture medium (marinebroth, MB) at a concentration of 0.3% or 0.15% (v / v), Pseudomonas vesicularis (MA103) and Aeromonas salmonicida (MAEF108) were inoculated therein, and cultured at 25° C. for 24 hours, so that these marine strains were induced to produce glycolytic enzymes. Then, the culture is separated, the bacteria are removed, the supernatant containing the saccharolytic enzyme is obtained, and the crude enzyme ...

Embodiment 3

[0063] Embodiment 3: Make the seaweed polysaccharide from Sargassum, Geliflower, Qinghai dish pass through the hydrolysis of crude enzyme liquid and through separation process

[0064] In this experiment, the polysaccharide samples from Sargassum, Geliflower, and Qinghai vegetable were hydrolyzed with the corresponding crude enzyme solution to produce oligosaccharides, which were then separated to obtain hydrolyzed fractions of seaweed oligosaccharides in different molecular weight ranges. In short, in the seaweed polysaccharide solution from Sargassum, Geliflower, and Qinghai vegetable obtained in Example 1, the crude enzyme solution S, crude enzyme solution G, and crude enzyme solution M (crude enzyme solution M) obtained in Example 2 were added respectively. The volume percentage of the seaweed polysaccharide solution is about 10%, for example, add about 100ml crude enzyme solution to the seaweed polysaccharide solution of about 1,000ml), and react at room temperature for 1-...

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Abstract

The invention discloses a method for preparing alga oligosaccharide with anticoagulation activity. Specifically, in the method, an alga oligosaccharide sample prepared from sargassum, gelidium amansii or green laver is hydrolyzed with an enzyme so as to obtain the alga oligosaccharide with anticoagulation activity, wherein the enzyme is produced by inducing an ocean strain of Pseudomonas vesicularis and / or Aeromonas salmonicida with the ocean polysaccharide from sargassum, gelidium amansii or green laver.

Description

[0001] Cross-Referenced Related Applications [0002] This application claims the priority of the Chinese patent application submitted on September 23, 2014, with the application number 201410489425.3, and the title of the invention is "A Preparation Method of Seaweed Oligosaccharides with Anticoagulant Activity", and its specification is quoted in its entirety incorporated into this article. technical field [0003] The invention relates to a method for preparing seaweed oligosaccharides with anticoagulant activity. The present invention also relates to a seaweed oligosaccharide with anticoagulant activity prepared by the method, and a composition containing the seaweed oligosaccharide. Background technique [0004] Some seaweed polysaccharides and oligosaccharides have been studied for many years because of their complex structure and the importance of their physiological activities. Many polysaccharides and oligosaccharides with sulfate groups have unique physiological a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/14C07H3/06A61K31/702A61P7/02C12R1/38C12R1/01
Inventor 潘崇良江孟灿吴绍祺李樵黄立瞱陈柏璇
Owner 潘崇良
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