Denitrification bactericide and use method thereof

A nitrogen bacteria agent and bacillus technology, which is applied in the field of wastewater treatment, can solve problems such as low denitrification efficiency, and achieve the effects of low operating cost, simple operation and environmental friendliness.

Active Publication Date: 2019-10-11
博瑞德环境集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a denitrification bacterial agent to solve the problem of low denitrification efficiency in the biochemical system of hydroxyethyl cellulose production wastewater in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Acquisition of Bio-augmented Bacteria Strains

[0044] The biosynthetic bacteria strains PR-DN1, PR-DN2, and PR-DN3 were isolated from activated sludge of a sewage treatment plant in Nanjing, and identified by 16SrDNA gene sequencing as Aeromonas, Alcaligenes, and Acinetobacter, respectively. It was deposited in China Center for Type Culture Collection (CCTCC) on April 15, 2019, May 20, 2019, and May 20, 2019. The preservation number of PR-DN1 is CCTCCM 2019255, and the preservation number of PR-DN2 is CCTCCM 2019372, the deposit number of PR-DN3 is CCTCCM2019373.

[0045] The composition of the screening medium is: 200 mg / L nitrate nitrogen, 1 g / L potassium dihydrogen phosphate, 1 g / L potassium dihydrogen phosphate, and 20 g / L agar. The activated sludge was serially diluted (diluted 10 6 、10 7 、10 8 times), absorb 0.1mL of the dilution and add it to the screening medium, spread it evenly with a glass rod, incubate at 33°C for 48h-72h, pick the three strains with th...

Embodiment 2

[0049] Preparation of Biosynthetic Bacteria

[0050] With the bioaugmentation agent bacterial strain that embodiment 1 obtains, Aeromonas ( Aeromonas sp. ) PR-DN1, Alcaligenes ( Alcaligenes sp. ) PR-DN2, Acinetobacter ( Acinetobacter sp. ) PR-DN3, Bacillus cereus ( Bacillus cereus. ) PR-DN4, microbacteria ( Exiguobacterium sp. ) PR-DN5, these 5 strains were inoculated into sterilized LB medium for activation, used as seed liquid, cultured at 30°C for 24-28h, and then the seed liquid of 5 strains were inoculated into sterilized LB Ferment in medium (the volume of seed liquid is 1%-5% of the volume of LB medium) and culture at 0°C for 36h-60h, and the number of viable bacteria obtained is 10 9 -10 11 cfu / mL of fermentation broth. Aeromonas ( Aeromonas sp. ), Alcaligenes ( Alcaligenes sp. ), Acinetobacter ( Acinetobacter sp. ), Bacillus cereus ( Bacillus cereus. ), microbacteria ( Exiguobacterium sp. ) according to the volume ratio of 30:17:17:20:16 to prepare ...

Embodiment 3

[0053] Preparation of Biosynthetic Bacteria

[0054] The preparation method is the same as that in Example 2, except that the fermented liquid of the above-mentioned five bacterial strains is prepared into a mixed liquid according to the volume ratio of 40:10:10:20:20.

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Abstract

The invention provides a denitrification bactericide. The bactericide comprises Aeromonas sp. PR-DN1, Alcaligenes sp. PR-DN2, Acinetobacter sp. PR-DN3, Bacillus cereus. PR-DN4 and Exiguobacterium sp.PR-DN5. The bactericide can be used for wastewater treatment after being put into wastewater containing nitrate nitrogen and nitrite nitrogen to be evenly mixed. The denitrification bactericide is anefficient bactericide which is greatly screened and compounded according to the characteristics of the wastewater, the nitrate nitrogen and nitrite nitrogen in the wastewater can be effectively removed, operation is simple, the treatment cycle is shortened, and the denitrification bactericide is friendly to the environment and low in operation cost.

Description

technical field [0001] The invention relates to a denitrification bacterial agent and a method for using the same, and relates to the field of waste water treatment. Background technique [0002] The main source of all nitrogen compounds on Earth is nitrogen. Human activities such as mining, industrial and agricultural nitrogen fixation, and excessive discharge of nitrogen-containing daily necessities into the environment seriously interfere with the natural nitrogen cycle and cause nitrogen pollution. All the intermediate products of the nitrogen cycle have adverse effects on humans and the environment. Among them, ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen can induce eutrophication in water bodies. Nitrite nitrogen is a carcinogen and can pass through ammonium nitrite. Nitrate nitrogen can oxidize ferrous ions into ferric ions, causing methemoglobinemia in infants. Only by completely removing the nitrogen in the water body can the adverse effects of nitroge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01C12R1/05C12R1/085C02F103/38
CPCC12N1/20C12N1/02C02F3/34C02F2103/38
Inventor 李丽严月根吴华明宗春香郭娜蔡旅程
Owner 博瑞德环境集团股份有限公司
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