Device for cell membrane permeabilization migration test and forming mould for manufacturing device

Abstract

The invention discloses a device for a cell membrane permeabilization migration test and a forming mould for manufacturing the device, belonging to the devices for cell experiments. The device is characterized by comprising a disc-shaped culture dish filled with gelatinized agarose, a cell hole defined by the gelatinized agarose in the culture dish, a chemotaxin hole defined by the gelatinized agarose in the culture dish, a migration channel defined by the gelatinized agarose in the culture dish and communicating the cell hole and the chemotaxin hole, and a macromolecule filter membrane blocked in the migration channel. The forming mould for manufacturing the device is characterized by comprises a base plate, and a mould body, wherein two sides of the base plate are provided with arc-shaped vertical edges, the convex-column-shaped mould body is arranged in the center of the base plate, and manufactured according to the shapes and sizes as well as the structures of the cell hole, the chemotaxin hole, the migration channel and the macromolecule filter membrane. Through trial, compared with the prior art, the device and the mould have the advantages that the migration movement of the cells passing through the porous polyester fiber membrane can be directly observed, the number of the cells successfully passing through the porous polyester fiber membrane can be accurately counted, and the cells successfully passing through the porous polyester fiber membrane can be conveniently collected for follow-up experiments.

Description

technical field [0001] The invention belongs to a device for cell migration experiment, in particular to a device for cell permeation membrane migration experiment and a model for making. Background technique [0002] Existing methods and devices for cell migration experiments mainly include the traditional cell migration experiment under agarose and the in vitro cell invasion device based on Transwell. [0003] For example, the existing technical document 1, whose publication number is CN102181397A, and whose patent number is ZL201110039718.8, discloses a cartilage invasion model of laryngeal squamous cell carcinoma, "It includes an in vitro experimental model of laryngeal squamous cell carcinoma invasion, laryngeal squamous cell carcinoma cartilage invasion Semi-in vitro test model and in vivo test model of cartilage invasion of laryngeal squamous cell carcinoma. As a preference, the construction method of the in vitro test model of laryngeal squamous cell carcinoma invasi...

AI Technical Summary

Problems solved by technology

[0006] (1) It is not possible to visually observe the migration of cells and count the number of cells

[0007] (2) The cells on the membrane may be lost during the process of fixed staining, thus affecting the accuracy of the experiment

[0008] (3) After the experiment, only the cells on the membrane can be fixed and stained for observation, and it is difficult to collect cells for subsequent experiments

[0011] (1) Only the migration of cells under agarose can be observed, without the process of transmembrane

[0012] (2) The cells on the culture dish are more likely to be lost during the process of fixed staining, and a large number of cells may be displaced during the process of removing the agarose, thus affecting the accuracy of the experiment

[0013] (3) After the experiment, only the cells on the culture dish can be fixed and stained for observation, and it is difficult to collect the cells for subsequent experiments

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