Breeding method of earliest-maturing broccoli selfing line

A technology of broccoli and inbred lines, which is applied in the field of plant cultivation, can solve the problems that the hybridization rate of hybrids is difficult to reach 100%, it takes a lot of manpower and time, and the flower organs of broccoli are small, so as to achieve good seed setting, The effect of shortening the breeding period and strong black rot

Inactive Publication Date: 2015-12-02
ZHENJIANG SUIHAN AGRI
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Problems solved by technology

Due to the small flower organs of broccoli, it takes a lot of manpower and time to reproduce the parents when using self-incompatible lines to prepare hybrids. 100%, the use of cytoplasmic male sterile lines for seed production can overcome the above defects, so breeders actively carry out the selection of broccoli cytoplasmic male sterile lines

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  • Breeding method of earliest-maturing broccoli selfing line

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Embodiment 1

[0019] During the flowering period at the beginning of March in the first year, the excellent single plant of Guangdong kale was artificially detasseled, and the pollen of the excellent single plant of Lilu broccoli was smeared on the stigma for hybridization, and the gauze bag was used for isolation. At the end of April Harvest the seeds and get the hybrid F1; prepare 150 pots (upper mouth Φ20cm, bottom Φ10cm, height 20cm) in early May, fill them with special nutrient soil for vegetables, tap holes in the pots, the depth of the holes is 1cm, and sow 1 grain in each hole (F1), cover the soil after planting, water thoroughly, keep the soil moist, and put them in the smart greenhouse, with temperature control equipment such as shading nets, water curtains, fuel heating equipment, etc., at 22-25°C during the day and 18-20 at night ℃. In mid-May, when the plant has 2 leaves and 1 heart, spray 20ml of "One Spray Green" (20ml / bag, FeMnZnB≧20g / l, amino acid≧100g / l, spray diluted 500 ...

Embodiment 2

[0027] In early March of the first year, an excellent single plant of early-maturing Broccoli Lvxiu cytoplasmic male sterile plant at the flowering stage was used as the female parent, and the pollen of the excellent individual plant of the inbred line 10-1-7-2-1 was smeared at the flowering stage for hybridization, and Isolate with gauze bags, harvest the seeds at the end of April, and get the hybrid F1; prepare 60 pots (top Φ20cm, bottom Φ10cm, height 20cm) in early May, fill them with special nutrient soil for vegetables, and tap acupuncture points in the pots. 1 cm deep, sow 1 grain per hole (F1), sow 40 grains, 10-1-7-2-1 sow 20 grains, cover with soil after sowing, water thoroughly to keep the soil moist, and place them in a smart greenhouse, through shading Temperature control equipment such as nets, water curtains, and fuel heating equipment, 22-25°C during the day and 18-20°C at night. In mid-May, when the plant has 2 leaves and 1 heart, spray 20ml of "One Spray Green...

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Abstract

The invention discloses a breeding method of an earliest-maturing broccoli selfing line. The breeding method particularly comprises the following steps: directly sowing seeds in pots and intensively placing the pots in an intelligent greenhouse; controlling high temperature in summer by using a water curtain, an air blower and a light shading net, and improving low temperature in winter by using fuel oil temperature control equipment; at a bolting stage, utilizing an atomization device in the greenhouse to keep the relative air humidity in a range from 70% to 80% at 10:30-14:30 in daytime; identifying the disease resistance of stalk break and black rot and the intensity of stress resistance in fields so as to ensure strong disease resistance and stress resistance of superior single plants; and at a bolting flowering period, carrying out pollination at a flowering phase, and bagging and selfing at the flowering phase. The obtained broccoli selfing line can be used as a backcross transformation male parent of a broccoli cytoplasmic male sterile line or a male parent of a cytoplasmic male sterile line to prepare a first-filial generation; and meanwhile, the breeding age limit is greatly shortened in practice, the time and working amount are saved, and the breeding method has the characteristics of high working efficiency and strong operability.

Description

technical field [0001] The invention relates to a method for breeding broccoli, in particular to a method for selecting an inbred line of very early-maturing broccoli, and belongs to the technical field of plant cultivation. Background technique [0002] Broccoli has the characteristics of compact plant type, short growth period, green color, small and loose flower ball at the top, long flower stem, strong germination ability of side branches, good taste, suitable for dense planting, strong stress resistance and disease resistance. It is becoming more and more popular with growers and consumers. Broccoli is eaten with the fat and tender small flower buds and stems at the top. It is rich in vitamins A, B, C, E, protein, anthocyanins and anticancer substances. The content of anticancer substances is high. It is a delicious and healthy food. A perfect combination of high-quality, high-grade functional green vegetables. It combines the advantages of broccoli and kale, and its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 秦文斌戴忠良胡诗建吴国平姚悦梅潘永飞张振超曹雪陈智超肖燕
Owner ZHENJIANG SUIHAN AGRI
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