Plant rhizosphere soil and rhizosphere exudate collecting device and method

Abstract

The invention discloses a plant rhizosphere soil and rhizosphere exudate collecting device. The device comprises a rhizosphere observation box, a collector and a non-rhizosphere-soil test tube, wherein the rhizosphere observation box comprises a root growth chamber and a collecting chamber, and a vertical separation net is arranged between the root growth chamber and the collecting chamber; the collector is a hollow tube with two ends open, sealing nets A are arranged at the two ends of the collector respectively, the two ends of the non-rhizosphere-soil test tube are open, and a sealing net B is arranged at one end of the non-rhizosphere-soil test tube. The invention further discloses a plant rhizosphere soil and rhizosphere exudate collecting method. By the adoption of the device and method, plants can be studied without disturbing plant roots or damaging the plant rhizosphere environment under the soil culture condition, secretion and absorption of the rhizosphere of a soil culture plant can be dynamically monitored and analyzed for different root positions and different time periods under the controllable condition, and rhizosphere soil and rhizosphere exudates can be obtained at the same time.

Description

technical field [0001] The invention relates to the field of biology, in particular to a device and method for collecting plant rhizosphere soil and rhizosphere exudates. Background technique [0002] At present, the collection of rhizosphere exudates is mainly concentrated in hydroponics, and there are also matrix culture collection methods and soil culture methods. The rhizosphere environment and plant physiological conditions are quite different between the hydroponics method and the matrix cultivation method and the soil cultivation conditions in which the plants actually grow. The traditional soil culture method is to take out the root system of the plant and rinse it with distilled water, and use the rinse solution as the rhizosphere exudate. During the whole operation process, the root system is very vulnerable to damage, and the contents and wound fluid of the root system itself will damage the plant itself. root exudates. According to different research purposes a...

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Problems solved by technology

For example, the Chinese patent CN1963437A has designed a continuous rhizosphere exudate collection device through anion and cation resins, but the design structure is more complicated, it is not easy to place, and it can only distinguish the root exudation of shallow and deep parts.

Chinese patent CN102776662A provides a collection device for xerophyte rhizosphere exudates, which can only be used for the collection of xerophyte rhizosphere exudates

[0003] To study the rhizosphere environment of plants, it is first necessary to obtain rhizosphere soil samples, but it is difficult to distinguish between rhizosphere and non-rhizosphere actually, because there are gradient changes in the properties of the root system

It is technically difficult to accurately distinguish the root-soil interface and collect soil samples under normal conditions

At present, the methods of obtaining rhizosphere soil mainly include field rhizosphere soil collection method and indoor simulation cultivation method. The former is too rough to distinguish rhizosphere soil and root surface soil, and it is difficult to show the true status of rhizosphere soil and root system.

The latter is widely used, mainly including horizontal root surface container culture method and vertical root surface container culture method. The horizontal root surface container culture method was first designed and proposed by Kuchenbuck and Jungk (1982). After the lower part of the soil has been in contact with the soil for a certain period of time, use a slicer or bare hands to cut the soil into a thin layer of 1mm thickness for analysis; Qin Shengwu (1984) first used the improved vertical root surface container culture method to measure the nitrogen fertilizer in the rice rhizosphere. The improved method can arbitrarily select root segments of different parts and different growth stages of plants to study the nutrient transfer status of the root-soil interface, but the soil samples obtained each time are limited, and multi-item determination cannot be performed at the same time

[0004] In summary, these methods can only have certain reliability for the specific research carried out, and there are certain limitations. They cannot realize the dynamic monitoring and analysis of the exudation-absorption process in the plant rhizosphere at different locations and at different times under controllable conditions.

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