Method for automated evaluation of incubated immunoblot strips
A technology of immunoblotting and evaluation unit, which is applied in the direction of material analysis by observing the influence of chemical indicators, analysis by chemical reaction of materials, and material analysis by optical means, which can solve problems such as difficulty in image evaluation.
Active Publication Date: 2015-12-09
EUROIMMUN MEDIZINISCHE LABORDIAGNOSTIKA
View PDF3 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Also note that even single shots of western blot strips with a fixed exposure time, with defined illumination and / or after a defined drying time do not always provide an optimal picture with regard to the background color or The ratio between the color and the background color, or the image is overlit, which eventually makes the image evaluation difficult
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0020]The drawing shows a camera 2 arranged above an incubation tank 3 with a plurality of incubation wells 4 , which takes pictures of immunoblotting strips 5 located in the wells 4 and transmits them in digital form to the evaluation unit 1 . The immunoblotting strips 5 arranged in the respective wells 4 have been incubated and at least partially dried. It is clearly visible that the different immunoblot strips 5 have staining in the form of bands 6 at different positions. Antigens are present on the surface of the test strip 5 at these positions, which react positively with the antibodies of the patient sample examined. In this way, a diagnosis recommendation can be generated which gives information about how likely it is that the patient suffers from a certain disease. It should be expressly pointed out that it is not important for the described device or the implemented method whether only one incubation well is filled with one blot strip or whether there are blot strips...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a device and a method for automated evaluation of incubated immunoblot strips (5). What is essential for such immunoblot strips (5) is that these have regions coated with antigens, which regions discolour under the formation of so-called bands (6) as soon as a binding occurs, in particular between the antigen and an antibody present in a patient sample. Within the scope of the described technical solution, a camera (2) is initially used to make recordings of the surface of an incubated immunoblot strip (5) and these recordings are transmitted in digitized form to an evaluation unit (1). Finally, the discolourings, which occurred in the form of bands (6), are detected and at least partly quantified in the evaluation unit (1), such that a diagnosis suggestion is generated taking into account the number of bands (6) and the colour strength thereof. The described technical solution is distinguished by virtue of the camera (2) being used in each case to make at least two recordings at different times and / or under different recording conditions.
Description
technical field [0001] The present invention relates to a device and method for automated evaluation of incubated immunoblot strips. What is important for such a Western blot strip is that it has antigen-coated regions which, in the event of binding, in particular between the antigen and the antibodies present in the patient sample, form the so-called The belt case is dyed. Within the scope of the described technical solution, the surfaces of at least two incubated immunoblot strips are first photographed by means of a camera, and the photographs are then transmitted in digital form to the evaluation unit. Finally, the staining in the form of bands is detected and quantified at least in part in the evaluation unit, so that a diagnostic recommendation is generated taking into account the number of bands and their color intensity. Background technique [0002] In the field of in vitro diagnostics, immunoblot strips such as Western blots or line blots are often used in order ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/59G01N21/17
CPCG01N21/253G01N21/78G01N21/8483G01N33/54386G01N33/543
Inventor W·迈尔T·舍佩尔R·卡夫卡
Owner EUROIMMUN MEDIZINISCHE LABORDIAGNOSTIKA