Pancreatic leakage detection agent based on divalent cobalt ions and application of pancreatic leakage detection agent

A detection agent, cobalt ion technology, applied in the field of medical detection, can solve the problems of not obvious color change, patient side effects, high dosage requirements, etc., to avoid stimulating pancreatic juice secretion, high sensitivity, and avoiding pancreatic fistula.

Inactive Publication Date: 2015-12-30
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the color range of litmus paper is 5.0 to 8.0, and the color change is not obvious in the physiological pH and pancreatic juice pH range. The pH of pancreatic juice is often lower than 8.0, and the litmus test paper is even more useless at this time. At the same time, litmus has a high requirement for the leakage of pancreatic juice. Sometimes, secretin is injected intravenously to stimulate the secretion of pancreatic juice. Leakage tends to be small (20 μl), which also greatly limits the use of litmus paper
Moreover, the safety of the litmus test paper is unknown, and may bring other side effects to patients, and it remains to be discussed whether it will actually be used clinically

Method used

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  • Pancreatic leakage detection agent based on divalent cobalt ions and application of pancreatic leakage detection agent
  • Pancreatic leakage detection agent based on divalent cobalt ions and application of pancreatic leakage detection agent
  • Pancreatic leakage detection agent based on divalent cobalt ions and application of pancreatic leakage detection agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Weigh 0.5gCoCl 2 Dissolve in 100mlH 2 O, stirred, and ultrasonically dissolved to obtain CoCl 2 solution; the CoCl 2 The solution is dripped onto the filter paper and dried to obtain the pancreas leakage detection agent of the present invention.

[0047] Configure 10mmol / L, 50mmol / L, 100mmol / L and 150mmol / L HCO 3 -solution (solvent is water), for detecting the detection effect of the pancreatic leakage detection agent that embodiment 1 makes:

[0048] a) According to figure 1 as shown ( figure 1 "+" means drop), when only 50μl H 2 When O, there was no significant change, and it was used as a control group;

[0049] When the pancreatic leak detection agent was not exposed to 3% H 2 o 2 Water solution pretreatment, only drop 50μl 50mmol / L HCO on the surface of the pancreatic leak detection reagent 3 - solution, compared with the control group, the pancreatic leakage detection agent did not change significantly; similarly, in other without 3% H 2 o 2 Add 50μl ...

Embodiment 2

[0059] Weigh 0.5gCoCl 2 Dissolve in 100mlH 2 O, stirred, and ultrasonically dissolved to obtain the pancreatic leakage detection agent of the present invention.

[0060] Utilize the pancreas leakage detection agent that embodiment 2 makes to carry out following experiment:

[0061] Such as Figure 4 as shown,

[0062] The pancreatic leakage detection agent prepared in Example 2 itself is red.

[0063] When only 20μl 100mmol / L NaHCO was added 3 In aqueous solution, the color of the originally red pancreatic leak detection reagent turns blue-gray.

[0064] When adding 20μl 100mmol / L NaHCO at the same time 3 aqueous solution and 20 μl 3% H 2 o 2 In aqueous solution, the color of the originally red pancreatic leak detection reagent becomes navy blue.

[0065] The above results show that the pancreatic leakage detection agent prepared in Example 2 was tested by H 2 o 2 After pretreatment, HCO 3 - Causes a significant discoloration of the pancreatic leak detector to det...

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Abstract

The invention relates to the technical field of medical detection, in particular to a pancreatic leakage detection agent based on divalent cobalt ions and application of the pancreatic leakage detection agent. The pancreatic leakage detection agent is a solution containing divalent cobalt compound or is a water absorption material loaded with divalent cobalt compound, and is prepared by dissolving divalent cobalt compounds in solvent to prepare a solution or dropwise adding the divalent cobalt compounds to the water absorption material after the divalent cobalt compounds are dissolved in solvent. The divalent cobalt compounds are one of all cobalt-containing compounds which can generate divalent cobalt ions in solvent through ionization. By means of the pancreatic leakage detection agent, tissue liquid with pancreatic juice and tissue liquid without pancreatic juice can be distinguished through obvious color changes under the condition of low-dosage pancreatic juice, the high detection flexibility and the large detection range are achieved, and the pancreatic leakage detection agent is safe and reliable.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a divalent cobalt ion-based pancreatic leakage detection agent and its application. Background technique [0002] Pancreatic fistula is the most common and serious complication after pancreatic surgery, and it is one of the main causes of severe abdominal infection and hemorrhage after pancreatic surgery. The main cause of pancreatic fistula is that after surgical suturing, there are small cracks in some places due to surgical sutures, and pancreatic juice leaks (pancreatic leakage). However, pancreatic juice is a colorless and odorless liquid, so doctors cannot immediately and accurately judge whether there is pancreatic juice leakage after surgical suture. How to visualize pancreatic juice leakage becomes the key to preventing pancreatic fistula. [0003] The concentration of bicarbonate in pancreatic juice is high, the highest concentration can reach 140mmol / L, doze...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/79
Inventor 刘祥瑞周泉李素静苗毅陆子鹏蒋奎荣唐建斌申有青
Owner ZHEJIANG UNIV
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