Application of radix bupleuri polysaccharide in preparing medicine for preventing and treating vaccinum influenzae vivum and virus pneumonia

A technology of Bupleurum polysaccharides and influenza A, applied in antiviral agents, drug combinations, respiratory diseases, etc., to achieve the effect of acute lung injury and treatment of acute lung injury

Inactive Publication Date: 2016-01-13
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Looking at the research at home and abroad, there is no report on the efficacy and drug research of Bupleurum polysaccharides in the treatment of influenza and virus-induced acute lung injury.

Method used

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  • Application of radix bupleuri polysaccharide in preparing medicine for preventing and treating vaccinum influenzae vivum and virus pneumonia
  • Application of radix bupleuri polysaccharide in preparing medicine for preventing and treating vaccinum influenzae vivum and virus pneumonia
  • Application of radix bupleuri polysaccharide in preparing medicine for preventing and treating vaccinum influenzae vivum and virus pneumonia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Take 100g of Bupleurum herb, extract by cold immersion in 95% ethanol, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, concentrate, centrifuge, and the supernatant with S3 Chloroacetic acid removes free protein, centrifuges, and dialyzes the supernatant with water for 3 days, concentrates the dialysate to a small volume, adds ethanol to an alcohol content of 80%, centrifuges, precipitates and freeze-dries to obtain the total polysaccharide, and the product yield is more than 3%. The content exceeds 65%.

[0040] Table 1 shows the yield and content of total polysaccharides in Bupleurum chinensis.

[0041] The Bupleurum medicinal material is selected from Bupleurum chinense DC, Bupleurum scorzonerifolium Willd or Bupleurum smithiivar. parvifolium Wolff.

[0042] Table 1

[0043]

Embodiment 2

[0045] 24 KM mice (16-18g) were randomly divided into 4 groups (A, B, C, D) according to body weight: group A was the normal control group, group B was the H3N2 virus model group, and group C was the Bupleurum polysaccharide group (40mg / kg), D group is Bupleurum polysaccharide 20mg / kg, 6 in every group. Animals in all groups except group A were anesthetized with ether, and infected with 5LD by intranasal drip 50 30 μL of H3N2 virus solution, 30 μL of 1640 culture solution infected by intranasal drip in group A was used as a control; groups C and D were intragastrically administered 2 hours after infection with H3N2, and the doses were 40 mg / kg and 20 mg / kg respectively; at the same time, groups A and B were given 0.5 %CMC was administered intragastrically as a normal and virus control. Dosing once a day for four consecutive days. After 96 hours of H3N2 virus attack, the body weight was weighed, and the eyeballs were removed to collect blood. Carefully cut off the whole lung...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and in particularly relates to an application of radix bupleuri polysaccharide in preparing a medicine for preventing and treating flu and virus-induced acute lung injury. According to the invention, a total polysaccharide extract is separated and extracted from traditional Chinese medicine radix bupleuri, wherein the average yield of the product is 3%, and the content of polysaccharide exceeds 65%. The integral experiment proves that the radix bupleuri total polysaccharide extract has a remarkable treatment effect for the mouse acute lung injury induced by influenza A virus H3N2. Through an anti-inflammatory mechanism thereof, the radix bupleuri polysaccharide inhibits the release and activation of pro-inflammatory cytokines and chemotactic factors, promotes the release of the inflammatory cytokines, inhibits the replication of the virus, and alleviates empsyxis and pulmonary edema, thus reducing acute lung injury. The radix bupleuri polysaccharide can be used for preparing the medicine for preventing and treating vaccinum influenzae vivum and virus pneumonia.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medical application of total polysaccharide of Bupleurum bupleuri. It specifically relates to the use of Bupleurum polysaccharide in the preparation of medicines for preventing and treating influenza A and viral pneumonia. Background technique [0002] Viral pneumonia (viralpneumonia) is lung inflammation caused by a variety of viral infections, usually caused by the downward spread of upper respiratory tract viral infection. Influenza virus is the most common virus that causes pneumonia, and it also includes parainfluenza virus, cytomegalovirus, adenovirus, rhinovirus, coronavirus, and some enteroviruses, which can also cause viral pneumonia. Influenza A viruses are important pathogens that cause respiratory diseases in humans and poultry. Influenza virus invades the bronchiolar epithelium and causes bronchiolitis, and the infection spreads to the lung interstit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P31/16A61P31/12A61P11/00
Inventor 陈道峰朱海燕力弘史训龙卢燕周珮
Owner FUDAN UNIV
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