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A method for increasing the content of cordycepin in the fruiting bodies of Cordyceps militaris

A technology of cordycepin and grass seeds, which is applied in the field of artificial cultivation to increase the content of cordycepin in the fruiting body of Cordyceps militaris, can solve the problems of low content of cordycepin, and achieve the effect of increasing the content of cordycepin, which is easy to implement and flexible in implementation

Active Publication Date: 2017-12-22
江苏康能生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the current situation that the content of cordycepin in the fruiting bodies of Cordyceps militaris is generally low in the current scale production, and provides a method for increasing the content of cordycepin in the fruiting bodies of Cordyceps militaris, that is, changing the culture conditions at a specific stage of Cordyceps militaris cultivation, In order to increase the content of cordycepin in the fruiting body of Cordyceps militaris, this method can also be superimposed with the current existing technical solutions, and has novel creativity and wide application value

Method used

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  • A method for increasing the content of cordycepin in the fruiting bodies of Cordyceps militaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Prepare medium:

[0028] Its formula is: rice 35g, silkworm chrysalis powder 7g, nutrient solution 65ml, pH value 6-8; its nutrient solution formula is: sugar 800g, peptone 800g, potassium dihydrogen phosphate 45g, magnesium sulfate 90g, multivitamin 30g, water 65kg ;

[0029] (2) Sterilization:

[0030] Seal the prepared medium and place it in a high-pressure humid heat sterilizer at 123°C for 80 minutes;

[0031] (3) Vaccination:

[0032] After the temperature of the medium is as low as 18-25°C, insert the Cordyceps militaris under sterile conditions;

[0033] (4) Bacteria:

[0034] Place the inoculated culture medium in a cultivation room for cultivation at a temperature of 15-20°C and a humidity of 60-80%, and cultivate in dark for 4-8 days until the germs are completely developed;

[0035] (5) Fruiting body development management:

[0036] When the bacteria are completely given 250lux white light, 24 hours / day, keep the temperature at 15-20°C and humidity...

Embodiment 2

[0040] (1) Prepare medium:

[0041] Its formula is: rice 35g, silkworm chrysalis powder 7g, nutrient solution 65ml, pH value 6-8; its nutrient solution formula is: sugar 800g, peptone 800g, potassium dihydrogen phosphate 45g, magnesium sulfate 90g, multivitamin 30g, water 65kg ;

[0042] (2) Sterilization:

[0043] Seal the prepared medium and place it in a high-pressure humid heat sterilizer at 123°C for 80 minutes;

[0044] (3) Vaccination:

[0045] After the temperature of the medium is as low as 18-25°C, insert the Cordyceps militaris under sterile conditions;

[0046] (4) Bacteria:

[0047] Place the inoculated culture medium in a cultivation room for cultivation at a temperature of 15-20°C and a humidity of 60-80%, and cultivate in dark for 4-8 days until the germs are completely developed;

[0048] (5) Fruiting body development management:

[0049] When the germs are completely given 250lux white light, 24 hours / day, keep the temperature at 15-20°C and the humidit...

Embodiment 3

[0053] (1) Prepare medium:

[0054] Its formula is: rice 35g, silkworm chrysalis powder 7g, nutrient solution 65ml, pH value 6-8; its nutrient solution formula is: sugar 800g, peptone 800g, potassium dihydrogen phosphate 45g, magnesium sulfate 90g, multivitamin 30g, water 65kg ;

[0055] (2) Sterilization:

[0056] Seal the prepared medium and place it in a high-pressure humid heat sterilizer at 123°C for 80 minutes;

[0057] (3) Vaccination:

[0058] After the temperature of the medium is as low as 18-25°C, insert the Cordyceps militaris under sterile conditions;

[0059] (4) Bacteria:

[0060] Place the inoculated culture medium in a cultivation room for cultivation at a temperature of 15-20°C and a humidity of 60-80%, and cultivate in dark for 4-8 days until the germs are completely developed;

[0061] (5) Fruiting body development management:

[0062] When the bacteria are completely given 250lux white light, 24 hours / day, keep the temperature at 15-20°C and humidity...

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Abstract

The invention relates to a method for increasing the content of cordycepin in cordyceps militaris sporocarp. It is required that high-temperature stress treatment is adopted in the particular period of culture of the cordyceps militaris sporocarp, namely from the 40th day to the 50th day, the culture temperature is raised to 24-30 DEG C from 15-20 DEG C to be kept for 10-20 days, harvesting is conducted on the day ranging from the 55th day to the 65th day, and the cordyceps militaris sporocarp with the cordycepin content reaching 0.5% can be obtained. According to the method, from the 40th-50th day of cordyceps militaris sporocarp culture, high-temperature stress treatment is adopted; meanwhile, strong white light or strong blue light stimulation treatment is assisted, and the content of the cordycepin in the cordyceps militaris sporocarp can be further increased and can reach 0.6%-0.9% and 0.9%-1.5%. The method has great innovativeness and application value.

Description

technical field [0001] The invention relates to the technical field of artificial cultivation of Cordyceps militaris, in particular to an artificial cultivation method for increasing the content of cordycepin in fruit bodies of Cordyceps militaris. Background technique [0002] Cordyceps militaris ( Cordyceps militaris (L.)Link ) belongs to Eumycota, Ascomycota, Ascomycotinia, Sordariomycetes, Hypocreomycetidae, Hypocreales, Cordyceps family (Cordycipitaceae), Cordyceps ( Cordyceps ). Cordyceps militaris, also known as Cordyceps militaris, has similar bioactive components to Cordyceps sinensis, mainly including cordycepin, cordycepic acid, polysaccharides, ergosterol, amino acids, nucleosides, etc. Its efficacy is similar to that of Cordyceps sinensis, and it is a good substitute for Cordyceps sinensis. And the content of cordycepin is also higher than that of Cordyceps sinensis. [0003] Cordycepin (3′-deoxyadenosine), also known as cordycepin, is the first nucleoside ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04A01G7/06
CPCA01G7/06A01G18/00
Inventor 左松窦小佳王琨
Owner 江苏康能生物工程股份有限公司
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