Cordyceps sinensis sesames

A technology of sesame and Cordyceps militaris, applied in the field of bioengineering, can solve the problems affecting the remarkable efficacy of Cordyceps sinensis, and achieve the effect of easy realization of large-scale production, controllable quality and wide application prospects

Inactive Publication Date: 2015-08-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interestingly, cordycepin is mainly present in Cordyceps militaris, and its content in Cordyceps sinensis is very small, but the absence of cordycepin did not affect the significant efficacy of Cordyceps sinensis

Method used

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  • Cordyceps sinensis sesames

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The DS56-Ⅲ twin-screw extruder produced by Jinan Juxian Machinery Equipment Co., Ltd. was used to process sesame. The sesame used was commercially available, and its moisture content was determined to be 15.2% by a moisture analyzer. The feed rate was set at 120 kg / h. Table 1 lists the temperature combination and expansion degree of the three sections. It can be seen from Table 1 that when the temperatures in the preheating zone, transportation zone, and curing zone are 60, 160, and 190°C respectively, the expansion degree of sesame is the highest. After further drying, the expansion degree at different water contents was investigated. It can be seen from Table 2 that the puffing effect is the best when the moisture content is about 14-18%, so the commercially available sesame can be directly used for puffing.

[0019] Table 1: The puffing degree of puffed sesame obtained from the combination of different temperatures in different sections of the extrusion puffing mac...

Embodiment 2

[0024] Inoculate the Cordyceps sinensis strain into a 250ml Erlenmeyer flask with 50ml medium, the composition of the medium is: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, culture for 96 hours , as seeds for inoculation.

[0025] Inoculate the Cordyceps militaris strain into a 250ml Erlenmeyer flask with 50ml of culture medium. The culture medium is composed of: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, and culture for 96 hours as seeds for inoculation.

[0026] Mix puffed sesame seeds with a puffing degree of 2.3 and sesame seeds with a mechanical crushing degree of 1 mm, totaling 50 g, add 40 g of distilled water, put them into a 500 ml Erlenmeyer flask, sterilize at 121 °C for 20 minutes, and inoculate each of the seed liquids of Cordyceps sinensis and Cordyceps militaris after cooling. 5ml, in a constant temperature and humidity incubator to control the ambient humidity to 90%, the temperature is 25°C, and...

Embodiment 3

[0030] Mix 30g of puffed sesame seeds with an expansion degree of 2.3 and 20g of sesame seeds with a mechanical crushing degree of 2mm, add 40g of distilled water, put them into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate the cultured Cordyceps sinensis and Cordyceps militaris seeds after cooling Each 5ml of liquid, in the constant temperature and humidity incubator, control environmental humidity 80%, temperature is 15 ℃, and after 20 days of fermentation, it is 5.8% to measure cordycepin content.

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Abstract

With sesames as a culture medium, cordyceps sinensis and cordyceps militaris are subjected to double-bacterial mixed solid fermentation. The invention belongs to the technical field of biological engineering. The sesames prepared through the double-bacterial bio-conversion have functions of health caring and are excellent in application prospect.

Description

technical field [0001] The method adopts Ophiocordyceps sinensis and Cordyceps militaris and uses sesame as a culture medium to carry out double-bacteria mixed solid-state fermentation, which belongs to the technical field of bioengineering. The preservation number of the bacterial strain Cordyceps militaris is CICC 14013, and the preservation number of the bacterial strain Ophiocordyceps sinensis is CICC 14088, and the nutrition and health sesame is obtained through double-bacteria mixed solid-state fermentation. Background technique [0002] Cordyceps belongs to Fungi, Ascomycota, Ascomycetes, Sordariomycetidae, Hypocreales, Clavcipitaceae . Most of the fungi of the genus Cordyceps are medicinal fungi, among which Ophiocordyceps sinensis is generally known and has the longest application history, and Cordyceps militaris has the most in-depth application research. [0003] According to literature research, Cordyceps sinensis was first recorded in "Yuewang Yaozheng" in 710...

Claims

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Application Information

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IPC IPC(8): A23L1/36A23L1/29A01G1/04A23L25/00A23L33/00
Inventor 蔡宇杰熊天真白玉管政兵廖祥儒张大兵
Owner JIANGNAN UNIV
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