A kind of culture method and culture medium of Cordyceps militaris with high selenium and high cordycepin content

A high cordycepin and cultivation method technology, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve problems such as being unsuitable for large-scale, safe, stable production, limited disposal of waste culture medium, existence of production process, etc. , to achieve the effect of normal shape, low cost and high commodity value

Active Publication Date: 2019-06-04
江苏康能生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent uses inorganic selenium such as sodium selenate as the selenium source, which has high toxicity and safety hazards in the production process, and the treatment of waste medium is limited, which is not suitable for large-scale, safe and stable production

Method used

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  • A kind of culture method and culture medium of Cordyceps militaris with high selenium and high cordycepin content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Activation of Cordyceps militaris strains

[0025] Prepare aseptic plate medium, select 0.5×0.5mm bacterial seed block to inoculate in the center of plate medium under aseptic operation conditions, seal with plastic wrap, and cultivate in dark at 19-21°C for 20 days.

[0026] (2) Preparation of Cordyceps militaris liquid seed

[0027] Use a 1000ml conical flask to prepare liquid seed nutrient solution: dehydrated potato powder 10g, sugar 10g, peptone 3g, yeast extract powder 3g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.5g, vitamin B1 0.5g, water 1000ml, PH 5- 6. Under aseptic operation conditions, select the plate species with round colony shape, select the viable bacterial species block at 0.5-2cm from the edge of the colony, and inoculate it in the liquid seed nutrient solution. Place in a shaker device for 6-7 days under shaking conditions.

[0028] (3) Cordyceps militaris hair fungus culture

[0029] Preparation of selenium nutrient solution:...

Embodiment 2

[0034] (1) Activation of Cordyceps militaris strains

[0035]Prepare aseptic plate medium, select 0.5×0.5mm bacterial seed block to inoculate in the center of plate medium under aseptic operation conditions, seal with plastic wrap, and cultivate in dark at 19-21°C for 30 days.

[0036] (2) Preparation of Cordyceps militaris liquid seed

[0037] Use 1000ml conical flask to prepare liquid seed nutrient solution: 15g dehydrated potato powder, 10g sugar, 4g peptone, 2g yeast extract powder, 0.5g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5g vitamin B1, 1000ml water, pH 5-6 . Under aseptic operation conditions, select plate species with a round colony shape, select viable bacterial species blocks at 0.5-2cm from the edge of the colony, and inoculate them in the liquid seed nutrient solution. Place in a shaker device for 6-7 days under shaking conditions.

[0038] (3) Cordyceps militaris hair fungus culture

[0039] Preparation of selenium nutrient solution: Weigh 1...

Embodiment 3

[0044] (1) Activation of Cordyceps militaris strains

[0045] Prepare aseptic plate medium, select 0.5×0.5mm bacterial seed block to inoculate in the center of plate medium under aseptic operation conditions, seal with plastic wrap, and cultivate in dark at 19-21°C for 20 days.

[0046] (2) Liquid seed culture of Cordyceps militaris

[0047] Use a 1000ml conical flask to prepare a liquid seed nutrient solution: 10g dehydrated potato powder, 15g sugar, 3g peptone, 3g yeast extract powder, 1g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5g vitamin B1, 1000ml water, pH 5-6. Under aseptic operation conditions, select plate species with a round colony shape, select viable bacterial species blocks at 0.5-2cm from the edge of the colony, and inoculate them in the liquid seed nutrient solution. Place in a shaker device for 6-7 days under shaking conditions.

[0048] (3) Cordyceps militaris hair fungus culture

[0049] Preparation of selenium nutrient solution: Weigh 1.3 ...

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Abstract

The invention relates to a high-selenium high-cordycepin-content cordyceps militaris culturing method and a culture medium thereof. Cordyceps militaris culture activation, liquid strain preparation, hairy fungus cultivation, and sporocarp growing and the like are carried out to realize cordyceps militaris culturing; a culture medium with a proper amount of organic selenium is applied and then high-selenium high-cordycepin-content cordyceps militaris with the complete form is obtained. The culturing method is simple based on advanced technologies and metabolic synthesis can be realized well. The employed culture medium uses food-grade organic selenium as a selenium source, so that effects of high security, environment protection, and high selenium conversion rate can be realized; the cordyceps militaris yield can be promoted; and the complete form can be guaranteed. Compared with the common cordyceps militaris, the cordyceps militaris cultured by the culturing method has advantages: the cordycepin content can reach over 1% and the selenium content can reach over 700mg / kg; and the industrial value and economic benefits are great.

Description

technical field [0001] The invention relates to the technical field of artificial cultivation of Cordyceps militaris, in particular to a method for simultaneously increasing the content of selenium and cordycepin in Cordyceps militaris. Background technique [0002] Cordyceps militaris ( Cordyceps militaris(L.)Link ), also known as Cordyceps sinensis, belongs to Eumycota, Ascomycota, Ascomycotinia, Sordariomycetes, Hypocreomycetidae, Hypocrea Order (Hypocreales), Cordyceps (Cordycipitaceae), Cordyceps ( Cordyceps ), a large medicinal fungus. The cordycepin contained in the fruiting body of Cordyceps militaris is 3′-deoxyadenosine (3′-deoxyadenosine), also known as cordycepin, which is the first nucleoside antibiotic isolated from fungi. Since the discovery and isolation of cordycepin in the middle of the 20th century, people have carried out research on its pharmacological efficacy and found that it has various effects such as anti-tumor, immune regulation, antibacterial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/20A01G18/00
CPCA01G18/00C12N1/14
Inventor 窦小佳王琨黄小力
Owner 江苏康能生物工程股份有限公司
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