Antibacterial composition for open type plant tissue culture and preparation method thereof

A plant tissue culture and composition technology, applied in the field of bacteriostatic compositions, can solve the problems of difficulty in large-scale popularization and application of tissue culture seedlings, strict requirements for sterile environment, and complicated operation.

Active Publication Date: 2016-01-20
北京阿格瑞斯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to solve the problem that the traditional plant tissue culture has strict requirements on the sterile environment, strict requirements on the operators, complex operation of the whole culture process and other factors, which make it difficult for tissue culture seedlings including strawberries to be produced on a large scale. The problem of popularization and application is to provide a safe and environmentally friendly, non-toxic, effective bacteriostatic composition for open culture in the rooting stage

Method used

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  • Antibacterial composition for open type plant tissue culture and preparation method thereof
  • Antibacterial composition for open type plant tissue culture and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 antibacterial composition

[0036] Weigh each component according to the following mass (unit: g): See Xuefei 18, Belladonna 4, Tongchenghu 5, add 540ml of water, decoct at 100°C for 2 hours; filter the decoction, discard the drug residue, and take the filtrate ( Abbreviated as liquid A), that is.

Embodiment 2

[0037]The preparation of embodiment 2 antibacterial composition

[0038] Weigh each component according to the following mass (unit: g): See Xuefei 20, Belladonna 3, Tongchenghu 6, add 580ml of water, decoct at 100°C for 3 hours, filter the decoction, discard the drug residue, and take the filtrate ( Abbreviated as liquid A), that is.

Embodiment 3

[0039] The preparation of embodiment 3 antibacterial compositions

[0040] Weigh each component according to the following mass (unit: g): See Xuefei 16, Belladonna 5, Tongchenghu 4, add 500ml of water, decoct at 100°C for 1 hour; filter the decoction, discard the drug residue, and take the filtrate ( Abbreviated as liquid A), that is.

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Abstract

The invention discloses an antibacterial composition for open type plant tissue culture and a preparation method thereof and belongs to the field of bacteriostatic agents for the open type plant tissue culture. According to the antibacterial composition, it is firstly found out through component screening tests that the antibacterial composition formed by matching caesalpinia cucullata, belladonna herbs and aristolochia fordiana can effectively restrain bacteria as well as remarkably increase the plant height of test-tube plantlets, promote growth of roots and increase the number of roots; it is further found out that a better synergic effect is achieved after the caesalpinia cucullata, the belladonna herbs and the aristolochia fordiana are matched according to the mass ratio of 16-20:3-5:4-6, particularly a remarkable synergistic synergia effect is achieved after the caesalpinia cucullata, the belladonna herbs and the aristolochia fordiana are matched according to the mass ratio of 18:4:5, pollution of a rooting cultivation medium can be effectively inhibited, and the effects of increasing the plant height of test-tube plantlets and promoting growth of roots and increasing the number of roots and the like are more remarkable; by means of the antibacterial composition, equipment investment of tissue culture is effectively lowered, the environment requirement is low, operation is simple and benefits are high.

Description

technical field [0001] The present invention relates to an antibacterial composition applied to tissue culture, in particular to an antibacterial composition formulated with plant extracts for the rooting stage of open plant tissue culture, and the present invention further relates to the antibacterial composition The invention relates to a preparation method of a substance, which belongs to the field of bacteriostats for open plant tissue culture. Background technique [0002] At present, tissue culture technology is one of the most commonly used technologies to solve strawberry virus diseases, which can restore the original good traits of strawberry seedlings and achieve the purpose of high quality and high yield. In recent years, the factory production of strawberry tissue culture seedlings has developed rapidly in China, and has achieved remarkable economic and social benefits. Because the traditional plant tissue culture has strict requirements on the sterile environme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/38A01N65/44A01P1/00A01P21/00A01H4/00
Inventor 蔡家锟李金艳赵东平朱珂佳
Owner 北京阿格瑞斯生物技术有限公司
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