Unlock instant, AI-driven research and patent intelligence for your innovation.

A slow feature-based cell division recognition method and its recognition device

A cell division and identification method technology, applied in the field of slow feature-based cell division identification methods and identification devices, can solve the problems of reducing the cell division identification rate, strong noise in microscope images, high computational complexity, etc., and achieves easy identification and tracking. Effects of processing, reduced computational complexity, increased capability

Active Publication Date: 2019-04-16
TIANJIN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1) The existing shape or structure feature extraction methods require accurate segmentation of cell regions, but microscope images often contain strong noise, making it difficult to accurately segment cells, so methods based on shape features usually have poor performance and generalization ability lower;
[0007] 2) Cell-based color feature extraction, because cells are relatively small, difficult to observe, and are seriously affected by background, staining, etc., so observation and tracking are relatively difficult, and there are problems such as high misjudgment rate and low work efficiency;
[0008] 3) Recognition methods based on phase models often require large-scale timing information to construct complex models. At the same time, the learning of the model has a high computational complexity, which reduces the speed of cell division recognition.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A slow feature-based cell division recognition method and its recognition device
  • A slow feature-based cell division recognition method and its recognition device
  • A slow feature-based cell division recognition method and its recognition device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] In order to make the feature extraction of cells more accurate, it can not only detect the edge of the image well, but also effectively reduce noise, see figure 1 , the embodiment of the present invention provides a method for identifying cell division based on slow features, the method comprising the following steps:

[0046] 101: Divide the cell image into a data set; randomly take half of the data in the positive and negative examples as the training set and the test set;

[0047] Wherein, before the cell image is divided into data sets, the method further includes preprocessing the cell image for grayscale normalization.

[0048] 102: Use unsupervised slow feature analysis to extract cell data to obtain slow feature functions;

[0049] 103: Calculate the cumulative square offset feature of the slow feature of the cell, and obtain the arrangement of the slow feature change rate from small to large;

[0050] 104: Use the method of model learning to detect the final ...

Embodiment 2

[0054] The scheme in embodiment 1 is described in detail below in conjunction with specific calculation formulas and examples, see the following description for details:

[0055] 201: Perform scale normalization preprocessing on all cell images;

[0056]Among them, each image sequence represents a cell division sequence, each image has a length W and a width H, and the sequence length is L. In order to simplify the problem, the embodiment of the present invention defaults that each sequence image to be split has been extracted, and the step of obtaining the split sequence through cell detection and tracking is no longer considered. So size normalization is to process each frame of image.

[0057] In the embodiment of the present invention, it is assumed that the size of the converted original cell image is s×s, and here s×s is uniformly set to 25×25 for illustration. There are no restrictions on the method of transformation.

[0058] 202: Divide the preprocessed cell image ...

Embodiment 3

[0092] Below in conjunction with specific Table 1 and Table 2, the scheme in Embodiment 1 and 2 is verified for feasibility, see the following description for details:

[0093] In this experiment, C2C12 mouse myoblasts commonly used in the prior art were used, and photographs were taken at five-minute intervals during the cell growth process by an optical microscope (ZeissAxiovert T135V). The image sequence has a total of 1013 frames, and the resolution of each image is 1392*1040. Randomly select 1000 frames of cell images, and after image preprocessing of the original cell data, use manual labeling to classify the cell data, and randomly select half of the labeled positive and negative examples to form the training set and test set respectively set, where the length of each image sequence is 21 frames, and the image size of each frame is 25*25. This method is then used for slow feature learning. Information and parameter settings on cell types, cell culture environments, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a slow feature-based cell division identification method and its identification device. The method includes: adopting an unsupervised slow feature analysis method, extracting cell data to obtain a slow feature function; calculating the cumulative square offset feature of the slow cell feature , to obtain the arrangement of slow feature change rates from small to large; use the method of model learning to detect the final cumulative square offset feature, and obtain the probability of mitosis in the process of cell data changing over time; if the output category is marked as 1, then The test data contains mitosis, if the output class is marked as 0, the test data does not contain mitosis. The device includes: a first acquisition module, a second acquisition module, a third acquisition module and an output module. The present invention reduces the difficulty of cell feature extraction, improves the accuracy of cell feature extraction, and provides a good solution for the identification and classification of subsequent dividing cells. The conditions are convenient for the identification and tracking of cells.

Description

technical field [0001] The invention relates to the field of image feature and pattern recognition, in particular to a slow feature-based cell division recognition method and a recognition device thereof, and in particular to the application of the slow feature to the field of cells. Background technique [0002] Cell biology is an important subject that studies cell structure, function, and living organisms. Cells promote the development of organisms through processes such as growth, division, aging, and death. Among them, cell division promotes the growth and development of organisms, as well as metabolism, which is of great significance to the process of cell growth. Then, how to quickly and accurately identify the process of cell division is of inestimable value to the study of cell changes and the development of things. [0003] In the research process of cell data, due to the large amount of original image data, it is usually not used as a feature to directly partici...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G06K9/00G06K9/62
CPCG06V20/695G06F18/285
Inventor 刘安安苏育挺李晓雪
Owner TIANJIN UNIV