72 results about "Mitotic nucleus" patented technology

A method for the automated analysis of digital images, particularly for the purpose of assessing mitotic activity from images of histological slides for prognostication of breast cancer. The method includes the steps of identifying the locations of objects within the image which have intensity and size characteristics consistent with mitotic epithelial cell nuclei, taking the darkest 10% of those objects, deriving contours indicating their boundary shape, and smoothing and measuring the curvature around the boundaries using a Probability Density Association Filter (PDAF). The PDAF output is used to compute a measure of any concavity of the boundary—a good indicator of mitosis. Objects are finally classified as representing mitotic nuclei or not, as a function of boundary concavity and mean intensity, by use of a Fisher classifier trained on known examples. Other uses for the method could include the analysis of images of soil samples containing certain types of seeds or other particles.

The present invention provides tricyclic compounds, pharmaceutically acceptable salts, prodrugs, solvates, or hydrates thereof, having antimitotic activity, anti-multidrug resistance activity, for example P-glycoprotein inhibition, and antitumor activity, and which inhibit paclitaxel sensitive and resistant tumor cells. Also provided are methods of utilizing these compounds for treating tumor cells and inhibiting mitosis of cancerous cells.

Disclosed herein are methods of inhibiting proliferation of a cancer cell or inducing apoptosis of a cancer cell, which does not overexpress N—CoR. Also disclosed herein are methods of inhibiting proliferation or inducing apoptosis of a cancer cell that overexpresses TCTP and methods for determining whether a compound is effective in inducing cell death.

The present invention discloses a rice large-grain gene GW2 and the application thereof, the GW2 gene can be used to control the grain size of the crop, improve the yield or quality of the crop, regulate the cycle duration of cellular mitosis, and used as a molecular marker to identify the species of the crop to be a large-grain one or a small-grain one. The present invention also discloses a method to ameliorate the crop. The gene GW2 has a wide prospect on high-yield breeding of crops such as rice.

Disclosed are compounds and pharmaceutically acceptable salts of Formula I:

wherein n, R₁, R₂, R₃, X, R₄, R₅, R₆, R₈, R₉, and Y are as defined herein. Compounds of Formula I are useful in the treatment of diseases and/or conditions related to cell proliferation and/or abnormal cell mitosis, such as cancer, inflammation and inflammation-associated disorders, and conditions associated with angiogenesis. Also disclosed are pharmaceutical compositions comprising compounds of the invention and methods of treating the aforementioned conditions using such compounds.

The invention relates to the field of medicinal chemistry, and discloses three crystal forms of cabazitaxel, i.e., an ester compound crystal form J of cabazitaxel, a hydrate crystal form G of cabazitaxel and a cabazitaxel crystal form I, and a preparation method of a novel crystal form of cabazitaxel. The novel cabazitaxel form has superior performance on the aspects of oral absorptivity, activation of metabolism and suppression of mitosis and interval cell function of cells, is weak in toxicity, has high storing and treating stability, and can be applied to preparation of a medicament for treating prostatitis.

Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor system can be manipulated positively or negatively to regulate mitosis in neuronal precursor cells. The ligand/receptor system involves PACAP, PACAP receptor, PAC₁, and related antagonists. The methods of regulation of the present invention may model be used to define cell cycle regulation in the developing neurons. The present invention may also be used to control or cure diseases related to or caused by damage to or destruction of neuronal cells.

Diseases and disorders of dermal tissue such as the skin, hair and nails are treated or prevented by administering one or more lectins, capable of binding to the surface of pathogenic microorganisms inhabiting the hair, skin, and nails, or of binding to the superficial tissues that comprise hair, skin, and nails. The lectins may be administered topically or subcutaneously to a patient infected with pathogenic microorganisms or in danger of being exposed to such pathogens. Lectins that stimulate cell mitosis may also be administered to accelerate wound healing and restore the appearance of age-wrinkled skin. Lectins that coagulate blood can be administered to assist in stopping bleeding from skin lesions. The lectins may be applied to the skin in a pharmceutically acceptable vehicle.

The present invention is directed to the use of mitotically and/or lethally inactivated stem cells for the repair of damaged organs and/or tissues. Stem cells are mitotically and/or lethally inactivated and transplanted into damaged tissue. Any form of ex vivo inactivation of stem cells may be used such that the stem cells cannot undergo mitosis or cell division before in vivo application. Mitotically and/or lethally inactivated stem may be used to ameliorate numerous disease, injury, traumatic, ischemic, aging, and/or degenerative conditions in different types of organs and/or tissues.

The invention provides a lymphocyte serum-free medium which comprises the following materials per liter: a basic culture medium, 10 mg of transferrin, 0.01 mg of sodium selenite, 110 mg of pyroracemic acid, 1.5 mg of ethanolamine, 10 mg of insulin, 10 to 30 mg of glutamine substitute, 300 mg of lipid-type bovine serum albumin, 0.001 mg of cupper sulfate, 0.0005 mg of ammonium meta-vanadate, 0.00005 mg of manganese chloride, 0.8 mg of zinc sulfate, 2 mg of Vitamin E, 100 mg of PHA, and 10 to 25 mg of an MHEPES/NaHCO3 buffering system. The invention further provides a preparation method and application to lymphocyte culture of the lymphocyte serum-free medium. The lymphocyte serum-free medium has the advantages that the repeatability of lymphocyte culture is high; poisoning and polluting of blood serum to lymphocyte are avoided; convenience is brought for differentiation of the lymphocyte, so as to obtain various mitosis phases.

The invention discloses a slow characteristic based cell division recognition method and a recognition device thereof. The method comprises the steps of extracting cell data by adopting a mode of unsupervised slow characteristic analysis so as to acquire a slow characteristic function; solving an accumulative square offset characteristic of the cell slow characteristic, and acquiring arrangement of variation rates of the slow characteristic from small to large; carrying out detection on the final accumulative square offset characteristic by using a method of model learning, and acquiring the probability of containing mitosis in the variation process of the cell data along with the time, wherein the test data contains mitosis if an output category label is 1, and the test data does not contain mitosis if the output category label is 0. The device comprises a first acquisition module, a second acquisition module, a third acquisition module and an output module. According to the invention, the difficulty of cell characteristic extraction is reduced, and the accuracy of cell characteristic extraction is improved, thereby providing good conditions for subsequent recognition and classification for dividing cells, and being convenient for recognition tracking and processing of the cells.

The invention belongs to the field of biomedicine and particularly relates to soluble human keratin and application thereof. The soluble keratin is obtained through the following process of cloning amiddle sequence of a human gene K37, constructing a recombinant vector, transforming bacteria, inducing expression of the recombinant protein and purifying the recombinant protein to obtain the soluble keratin. The soluble keratin has the characteristic of rapid swelling and can promote mitosis and migration of epidermal cells, and therefore the soluble keratin can be used for preparing biologicalmaterials or medicaments for promoting cell proliferation or migration, and plays a role in hemostasis and wound healing. Moreover, the soluble keratin causes no rejection phenomenon when being usedfor the human body, is safe to use and thus has a wide application prospect in the field of biomedicine.

The invention describes human genes involved in cell cycle progression, including mitosis and meiosis. The invention also relates to the use of these “cell cycle progression” genes and proteins in the modulation of cell cycle progression in cells and methods for identifying modulators of these genes or proteins and hence modulators of mitosis and meiosis.

The invention belongs to the field of functional foods and relates to a multi-component compounded functional solid drink for enhancing human immunity and a preparation method thereof. The functionalsolid drink comprises 20-30 parts of sorbitol, 15-25 parts of dried skim milk, 10-25 parts of resistant dextrin, 5-20 parts of xylooligosaccharide, 5-15 parts of yeast beta-glucan, 1-10 parts of bovine colostrum powder, 1-10 parts of orange powder, 1-10 parts of soybean peptide, 1-10 parts of fish collagen powder, 0.1-1 part of casein phosphopeptides, 0.1-1 part of composite amino acid powder and0.01-1 part of vitamin C. The functional solid drink is capable of improving human immunity from six aspects of stimulating immune cell regeneration, promoting mitosis of phagocyte, providing immunityactive factors, providing basic constitution substances for establishing cells of bodies, replenishing vitamin C and regulating intestinal tract health, and is high in security and remarkable in effect. The functional solid drink is prepared by using a modern process, multiple components are compounded, the components play a role of synergism, and the human immunity can be well improved; and compared with a conventional product in the market, the functional solid drink has a comprehensive replenishing function and good advantages.

A cell-free assay system, which reconstitutes components of the phosphatidyl-inositol 3-kinase-mediated insulin signaling pathway including phosphatidylinositol phosphate dependent kinase-2 (“PDK2”). Alternatively, a in vitro method for phosphorylating a protein kinase B on Serine 473 or Serine 474. The invention relates generally to an in vitro method of phosphorylating a protein kinase B (“PKB” or “Akt”), to an in vitro method of assessing insulin action, and to an in vitro method of identifying an agent or process that modulates insulin signaling or any cellular activity regulated or influenced by PKB, including cell growth, mitosis, apoptosis, fuel metabolism, and oncogenic transformation. Such an agent or process may be useful in treating insulin resistance, diabetes, obesity, cancer, and a number of other diseases.