Compound as well as preparation method and application thereof

A compound and plant technology, applied in the field of medicine, can solve the problem of less epimedium and achieve the effect of value-added promotion

Active Publication Date: 2016-03-09
JIANGSU KANION PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Epimedium is a plant belonging to the genus Epimedium of the Berberidaceae plant. It has the functions of nourishing the liver and kidney, strengthening muscles and bones, and boosting yang and essence. At present, there are relatively few basic research reports on Epimedium

Method used

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  • Compound as well as preparation method and application thereof
  • Compound as well as preparation method and application thereof
  • Compound as well as preparation method and application thereof

Examples

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preparation example Construction

[0029] The present invention also provides a preparation method of the compound shown in formula (I), comprising:

[0030] 1) extracting plants of the genus Epimedium to obtain a clear ointment;

[0031] 2) The obtained clear cream is separated to obtain the compound shown in formula (I).

[0032] According to the present invention, the present invention extracts plants of the genus Epimedium to obtain clear ointment; the plant of the genus Epimedium is preferably Epimedium, and in the extraction of the present invention, leaves of Epimedium are preferably used; The invention has no special limitation on the extraction method, any method known in the art for solvent extraction of Chinese herbal medicine components can be used, and the present invention preferably adopts the water alcohol precipitation method or alcohol extraction;

[0033] Specifically, in the present invention, plants of the genus Epimedium are crushed and sieved, mixed with water, extracted, the extract is ...

Embodiment 1

[0060] 1) 3.5kg of dried Epimedium leaves, pulverized and sieved, added 20 times the amount of water, extracted 3 times, each time for 1.5h, filtered, combined filtrates, concentrated under reduced pressure to obtain a clear cream; add ethanol to the clear cream to make the The amount of alcohol reaches 70wt%, standing at room temperature, filtering and concentrating, and recovering ethanol to obtain alcohol precipitation paste;

[0061] 2) Take the alcohol precipitation clear paste from step 1) and add purified water to dissolve it, let it stand at room temperature, take the supernatant and separate it through a D101 macroporous adsorption resin column, elute with 4 times the column volume of purified water, and discard the eluent , and then eluted with the same volume of 85% ethanol, and the 85% ethanol eluted part was collected; the 85% ethanol eluted part was passed through a D101 macroporous resin column, and gradient elution was carried out with different concentrations o...

Embodiment 2

[0068] 1) 3.5kg of dried Epimedium leaves, pulverized and sieved, added 20 times the amount of water, extracted 3 times, each time for 1.5h, filtered, combined filtrates, concentrated under reduced pressure to obtain a clear cream; add ethanol to the clear cream to make the The amount of alcohol reaches 70wt%, standing at room temperature, filtering and concentrating, and recovering ethanol to obtain alcohol precipitation paste;

[0069]2) Take the alcohol precipitation clear paste from step 1) and add purified water to dissolve it, let it stand at room temperature, take the supernatant and separate it through the HPD-100A macroporous adsorption resin column, elute with purified water 4 times the column volume, discard the washed Remove the liquid, then elute with the same volume of 85% ethanol, collect the 85% ethanol eluted part; the 85% ethanol eluted part is then passed through the HPD-100A macroporous resin column, and carry out gradient elution with different concentratio...

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Abstract

The invention provides a compound as well as a preparation method and application thereof. The compound has a structure shown as formula (I), and the compound can treat osteoporosis; moreover, by virtue of cell experiments, the compound is discovered to have an effect on promoting the proliferation of osteoblasts; and when the sample concentration is 10mu M, the proliferation rate can reach 11.24 percent.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a compound and its preparation method and application. Background technique [0002] Osteoporosis is a systemic skeletal disease characterized by osteopenia and degeneration of bone microstructure, resulting in increased bone fragility and susceptibility to fracture. With the acceleration of the aging society, the number of patients with osteoporosis is increasing at a rate of 5.2% per year. [0003] Epimedium is a plant belonging to the genus Epimedium of the Berberidaceae plant. It has the functions of nourishing the liver and kidney, strengthening bones and muscles, and boosting yang and essence. At present, there are relatively few basic research reports on Epimedium. Contents of the invention [0004] In view of this, the technical problem to be solved by the present invention is to provide a compound and its preparation method and application. [0005] The present inve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61P19/10
CPCC07H1/08C07H17/07
Inventor 萧伟李英张小强李露杨晶陆兆光吴云丁岗王振中
Owner JIANGSU KANION PHARMA CO LTD
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