Separation and purification method of steviol glycosides in stevia rebaudiana
A technology for the separation and purification of steviol glycosides, which is applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., and can solve problems such as cumbersome overall processes
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Embodiment 1
[0041]Mix 25g of stevia dry leaves with 200g of pure water for leaching, maintain the leaching temperature at 90°C, and leaching time for 40min, and then perform solid-liquid separation to obtain solid and liquid. Continue to mix the above solid with 200g of pure water, repeat the above leaching operation three times, keep the liquid after each leaching to obtain an extract, and finally add ethanol to make the ethanol ratio in the extract reach 10%. Perform HPLC (high performance liquid chromatography) test on the extract, such as figure 2 As shown, the results show that except for steviol glycosides St, RA and Rc in the extract, the peaks of other impurities are very high, indicating that the content of impurities in the extract is very high.
[0042] Load the above extract to a solid-phase extraction column packed with 30g of Generik RP packing that has been equilibrated with 10% methanol aqueous solution, and then use the pretreatment eluent, the first target eluent and th...
Embodiment 2
[0045] Mix 25g of stevia dry leaves with 200g of pure water for leaching, maintain the leaching temperature at 90°C, and leaching time for 40min, and then perform solid-liquid separation to obtain solid and liquid. Continue to mix the above solid with 200g of pure water, repeat the above leaching operation three times, keep the liquid after each leaching to obtain an extract, and finally add ethanol to make the ethanol ratio in the extract reach 10%. Perform HPLC (high performance liquid chromatography) test on the extract, such as figure 2 As shown, the results show that except for steviol glycosides St, RA and Rc in the extract, the peaks of other impurities are very high, indicating that the content of impurities in the extract is very high.
[0046] The above extract was loaded onto a FLASH column packed with 30 g of Generik RP filler equilibrated with 10% aqueous methanol in advance, and then the pretreatment eluent, the first target eluent and the second target eluent w...
Embodiment 3
[0049] Mix 25g of stevia dry leaves with 200g of pure water for leaching, maintain the leaching temperature at 90°C, and leaching time for 40min, and then perform solid-liquid separation to obtain solid and liquid. Continue to mix the above solid with 200g of pure water, repeat the above leaching operation three times, keep the liquid after each leaching to obtain an extract, and finally add ethanol to make the ethanol ratio in the extract reach 10%. Perform HPLC (high performance liquid chromatography) test on the extract, such as figure 2 As shown, the results show that except for steviol glycosides St, RA and Rc in the extract, the peaks of other impurities are very high, indicating that the content of impurities in the extract is very high.
[0050] Load the above extract to a liquid chromatography column packed with 30g of Generik RP filler equilibrated with 10% methanol aqueous solution, and then use the pretreatment eluent, the first target eluent and the second target...
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