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Method for preparing and screening alkaloid and anti-inflammation active ingredients in dactylicapnos scandens

An effective component, the technology of Zijinlong, applied in the field of drugs for the prevention and treatment of inflammatory diseases, can solve the problems of lack of research on the preparation and application of the effective components of Zijinlong, and achieve the goal of improving operability, good effect and low dosage Effect

Inactive Publication Date: 2016-06-01
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing literature is only for the separation and purification of Zijinlong extract to obtain 3 dither alkaloids, and lacks research on the preparation and application of Zijinlong effective components. Therefore, the present invention utilizes reasonable extraction, separation and The detection method finally provides a preparation method for effectively extracting, separating and enriching Zijinlong alkaloids and effective components of Zijinlong, and applying such components to drugs for the prevention and treatment of inflammatory diseases

Method used

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  • Method for preparing and screening alkaloid and anti-inflammation active ingredients in dactylicapnos scandens
  • Method for preparing and screening alkaloid and anti-inflammation active ingredients in dactylicapnos scandens
  • Method for preparing and screening alkaloid and anti-inflammation active ingredients in dactylicapnos scandens

Examples

Experimental program
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Embodiment 1

[0033] Preparation of alkaloids: Add 10 times, 8 times, and 6 times the amount of Zijinlong medicinal materials to reflux extraction with 60% ethanol for 3 times, each time for 1 hour, combine the extracts, recover ethanol under reduced pressure, and concentrate the medicinal solution. The concentrated solution is added to the column body equipped with D101 macroporous adsorption resin for column chromatography, followed by water (H 2 OFr.), 60% ethanol and 95% ethanol were eluted, the eluate was collected, and the 60% ethanol fraction and 95% ethanol fraction were combined (EtOHFr.) to obtain the water fraction (H 2 OFr.) and the combined ethanol fraction (EtOHFr.). After further concentrating the combined ethanol components, the alkaloid components were enriched, and the combined concentrated solution was subjected to neutral alumina column chromatography, and then eluted with chloroform and chloroform-methanol 1:1 in sequence, collected, The eluents were combined, concentr...

Embodiment 2

[0036] Preparation of alkaloids: Add 12 times, 9 times, and 6 times the amount of Zijinlong medicinal material to reflux extraction with 60% ethanol for 3 times, each time for 1 hour, combine the extracts, recover ethanol under reduced pressure, and concentrate the medicinal solution. The concentrated solution is added to the column body equipped with D101 macroporous adsorption resin for column chromatography, followed by water (H 2 OFr.), 60% ethanol and 95% ethanol were eluted, the eluate was collected, and the 60% ethanol fraction and 95% ethanol fraction were combined (EtOHFr.) to obtain the water fraction (H 2 OFr.) and the combined ethanol fraction (EtOHFr.). After further concentrating the combined ethanol components, the alkaloid components were enriched, and the combined concentrated solution was subjected to neutral alumina column chromatography, and then eluted with chloroform, chloroform-methanol 1:2 in sequence, collected, The eluents were combined, concentrated...

Embodiment 3

[0038] Preparation of alkaloids: Add 10 times, 7 times, and 4 times the amount of 60% ethanol to reflux extraction of Zijinlong medicinal materials for 3 times, each time for 1 hour, combine the extracts, recover ethanol under reduced pressure, and concentrate the drug solution. The concentrated solution is added to the column body equipped with D101 macroporous adsorption resin for column chromatography, followed by water (H 2 OFr.), 60% ethanol and 95% ethanol were eluted, the eluate was collected, and the 60% ethanol fraction and 95% ethanol fraction were combined (EtOHFr.) to obtain the water fraction (H 2 OFr.) and the combined ethanol fraction (EtOHFr.). After further concentrating the combined ethanol components, the alkaloid components were enriched, and the combined concentrated solution was subjected to neutral alumina column chromatography, followed by elution with chloroform, chloroform-methanol 2:1, and collected washed Deliquification, concentration under reduce...

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Abstract

The invention discloses a method for preparing and screening alkaloid and anti-inflammation active ingredients in dactylicapnos scandens. The method comprises the following steps: carrying out ethanol reflux extraction on a dactylicapnos scandens medicinal material, mixing extracting liquid, reclaiming ethanol in reduced pressure, and concentrating medicinal liquid; carrying out column chromatography on the concentrated liquid; eluting with chloroform, chloroform-methanol in a ratio of 1:1 and methanol-water in a ratio of 1:1 sequentially, collecting eluent, concentrating at reduced pressure, freezing and drying to respectively obtain a chloroform component (CHCl3 Fr.), a chloroform-methanol component (CHCl3-MeOH Fr.) and a methanol-water component (MeOH-H2O Fr.); and mixing the chloroform component and the chloroform-methanol component to obtain a dactylicapnos scandens alkaloid component, and screening the optimized anti-inflammatory active component (CHCl3-MeOH Fr.). The alkaloid and anti-inflammation active ingredients prepared and screened by adopting the method can be applied to treatment of inflammatory diseases in a safe concentration range, and can provide basis to deep development of the dactylicapnos scandens.

Description

technical field [0001] The present invention relates to the technical field of preparation and application of Zijinlong anti-inflammatory active components, in particular to a preparation and screening method of alkaloids and anti-inflammatory active components in Zijinlong and its use in medicines for preventing and treating inflammatory diseases . Background technique [0002] Zijinlong is the dry root tuber of Aconitum vilmorinianum Komvar.altifidum W.T.Wang, a plant of Ranunculaceae. It is a traditional medicinal material of the Miao nationality in Guizhou Province, which was recorded in the 2003 edition of "Quality Standards for Traditional Chinese Medicinal Materials and Ethnic Medicines of Guizhou Province". It has the effects of expelling wind and dehumidification, warming meridian and relieving pain. It is used for the treatment of injuries from falls, waist and knee pain, and sores. However, there are few reports on Zijinlong's clinical research in traditional C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P29/00C07G5/00
CPCA61K36/66A61K2236/333A61K2236/35A61K2236/39A61K2236/55C07G5/00
Inventor 董莉李勇军董永喜兰燕宇廖尚高王永林王爱民刘亭张晓红杨雅欣
Owner GUIZHOU MEDICAL UNIV
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