Fluorescent alga in-situ analysis device based on beam splitting and combining of broadband spectrum

Abstract

The invention relates to a fluorescent alga in-situ analysis device based on beam splitting and combining of a broadband spectrum. A beam shaping element, a broadband spectrum beam splitting element, a collimation shaping element and a movable light passing hole are sequentially arranged on a broadband spectrum light source emergent light path; a beam converging element, a broadband spectrum beam combining element, an excitation beam shaping element and a fluorescent excitation focusing element are sequentially arranged on a fluorescent excitation beam transmitted through the movable light passing hole; a detection converging element, a light filtering element and a fluorescent photoelectric detector are sequentially arranged on a fluorescent light beam path; the fluorescent photoelectric detector detects an alga fluorescent signal converged by the detection converging element and filtered by the light filtering element; a microscope objective, an imaging element and a two-dimension imaging photoelectric detector are arranged in a water body detection area to form an in-situ alga microscope imaging module; and a control analysis part is connected with a displacement control part, the fluorescent photoelectric detector and the two-dimension imaging photoelectric detector to obtain fluorescent information and image information and realize a passing-through function of movable selective-wavelength beams through the movable light passing hole, thereby realizing in-situ alga analysis.

Description

technical field [0001] The invention relates to an algae analysis device, especially a fluorescent algae in-situ analysis device based on wide spectrum beam splitting and combining, mainly used for algae research, algae evolution, aquatic biosphere, water quality monitoring, water channel analysis and evaluation, water environment Algae analysis in the fields of monitoring, water resource assessment, chemical analysis, lake engineering, scientific research and teaching, algal bloom monitoring, algal growth monitoring, etc. Background technique [0002] In modern botany, algae refers to a class of lower plants that have no differentiation of roots, stems, and leaves, no embryonic stage in individual development, and photosynthetic autotrophy. Algae are the oldest of all plants. Most algae live in water. Their structure is very simple, each visible individual has no distinction of roots, stems and leaves, it is a frond. Chinese scholars generally divide algae into 11 catego...

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Problems solved by technology

[0003] In the prior art, there are algae analysis devices, biological microscopes based on microscope technology and flow cytometers based on microscope systems. The prior technologies and devices have considerable advantages, but there are still some essential deficiencies: 1) based on biological microscopes and The algae analysis device of the flow cytometer needs to sample the tested water body to form a sample or import device, which cannot realize the in-situ analysis of the algae; 2) The tested algae has been separated from the original natural environment. Inconsistency leads to low precision and poor accuracy of algae analysis, which cannot reflect the real water algae situation and weak anti-interference; 3) Due to the wide variety of algae, in the detection and analysis of biological microscopes and flow cytometers based on image processing, real-time detection Insufficient performance, complex algorithm, difficult detection and analysis, can not carry out algae analysis and detection well; 4) The prior technology detects and analyzes based on microscopic images, and cannot detect and classify algae based on its own biological and fluorescent characteristics, which affects the purpose of algae analysis Realization, the amount of information obtained is low, and the scope of application is affected; 5) The prior device has low intelligence, poor flexibility, and low function expansion

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