Composite microelement fertilizer containing chlorella and paenibacillus, preparation and applications thereof
A technology of bacillus and compound micro-fertilizer, applied in the direction of organic fertilizers, can solve the problems of deterioration of soil structure, decline of soil microbial activity, reduction of soil organic matter, etc., and achieve the effects of improving the quality of agricultural products, improving disease resistance, and promoting plant growth
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Embodiment 1
[0029] Example 1 Preparation of compound micro-fertilizer containing Chlorella and Azotobacter
[0030] In this embodiment, the preparation of the compound micro-fertilizer containing Chlorella and Azotobacter bacillus was prepared according to the following steps:
[0031] 1) Expansion of Chlorella sp.:
[0032] ①Inoculate Chlorella sp. 34-2 in liquid medium (KH 2 PO 4 0.7g / L, K 2 HPO 4 0.3g / L, MgSO 4 0.3g / L, FeSO 4 0.1g / L, VB10.01mg / L, A5+Co + Trace element liquid 1ml / L and glucose 20g / L. A5+Co + Trace element liquid from H 3 BO 3 2.86g, ZnSO 4 ·4H 2 O0.222g, MnCl 2 ·4H 2 O1.8lg, CuSO 4 ·5H 2 O0.079g, Na2MoO 4 ·2H 2 O0.39g and Co(NO 3 ) 2 ·6H 2 O0.049g is dissolved in deionized water, and the volume is adjusted to 1000mL to prepare) and culture for 3-5 days;
[0033] ②Put the sewage obtained by diluting the farming sewage 8-10 times into the 10m×20m open-air breeding pond, and inoculate the Chlorella sp. cultivated in ① at a volume percentage of 10% inoculum. 34-2. After culturing ...
Embodiment 2
[0038] Example 2 Fertilization effect test of compound micro-fertilizer containing chlorella and nitrogen-fixing bacillus
[0039] In 2014 and 2015, in Changping District, Beijing, the compound micro-fertilizer containing Chlorella and Nitrogen-fixing Bacillus prepared in Example 1 was used at a dosage of 4 kg / mu during tomato planting. The results showed that nitrogen-fixing microorganisms were used alone Compared with the unused control, the inoculum increased the yield by 11%, and when the microalgae was used alone, the yield increased by 3.3%. The compound fertilizer (chlorella and nitrogen-fixing bacteria) increased the yield by 18.3% than the nitrogen-fixing bacteria alone, and increased the yield by 31% compared with the non-fertilizer The results are shown in Table 1, indicating that Chlorella and Azotobacter have a synergistic effect.
[0040] Table 1. Effects of different treatments on tomato yield
[0041] deal with Yield (kg / mu) (1) Control (no fertilization)3798.2 ...
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