DC (dendritic cell) induction activating agent and application thereof
A technology of activator and concentration, which is applied in the direction of cell culture active agent, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor antigen presentation performance, low cell proliferation rate, low induction maturation rate, etc., and increase the value-added rate and maturation rate, promote the improvement, and improve the effect of antigen presentation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0030] The application of the DC induction activator provided by the present invention in the method for inducing DC maturation comprises the following steps:
[0031] S1a, obtaining dendritic cell precursor mononuclear cells;
[0032] Draw 3-5ml of peripheral venous blood, dilute it with normal saline 1:1, then slowly add it to 3-5ml of Ficoll-Hypaque lymphocyte separation medium with a specific gravity of 1.077±0.001g / ml along the tube wall, and centrifuge at 1500r / min for 15 minutes , isolate and obtain peripheral blood mononuclear cells, and suspend the cells in RPMI1640 medium (purchased from Gibco) containing 10% fetal bovine serum, 100 U / mL penicillin, and 100 U / mL streptomycin at a concentration of 4×10 6 / ml, transferred to a six-well plate, 2ml / well, put the six-well plate at 37°C, 5% CO 2 Incubate in an incubator for 2 hours, remove the medium and suspended cells, put fresh RPMI1640 medium in each well, blow gently to blow up the cells on the wall, and collect the ...
Embodiment 2
[0040] The difference from Embodiment 1 is that in this embodiment,
[0041] Among the first inducers used,
[0042] The concentration of rhGM-CSF is 800U / ml,
[0043] The concentration of GM-CSF is 5ng / mL,
[0044] The concentration of IL-4 is 5ng / mL,
[0045] The concentration of rhIL-4 is 800U / ml,
[0046] and IL-13 at a concentration of 5 ng / mL;
[0047] In the second inducer,
[0048] The concentration of rhTNF-α is 800U / ml,
[0049] The concentration of gallic acid was 5 μg / ml,
[0050] Or the concentration of walnut green husk extract is 10μg / ml.
Embodiment 3
[0052] The difference from Embodiment 1 is that in this embodiment,
[0053] Among the first inducers used,
[0054] The concentration of rhGM-CSF is 1200U / ml,
[0055] The concentration of GM-CSF is 15ng / mL,
[0056] The concentration of IL-4 is 15ng / mL,
[0057] The concentration of rhIL-4 is 1200U / ml,
[0058] and IL-13 at a concentration of 15 ng / mL;
[0059] In the second inducer,
[0060] The concentration of rhTNF-α is 1200U / ml,
[0061] The concentration of gallic acid is 15μg / ml,
[0062] Or the concentration of walnut green bark extract is 30μg / ml.
[0063] In order to further verify that the DC-inducing activator provided by the present invention and its application have significant beneficial effects, the following experiments are set up for verification.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More