Liver cancer prognosis related serum miRNA markers and application of detection kit thereof

Abstract

The invention discloses liver cancer prognosis related serum miRNA markers and an application of a detection kit thereof. The tumor markers are serum miR-29a-3p and miR-192-5p which are related with human liver cancer patients. According to preliminary work, liver cancer related serum miRNA expression profiles containing miR-29a-3p and miR-192-5p and having obvious expression difference are screened out through high-throughput deep sequencing, and qPCR (quantitative polymerase chain reaction) proves and finally confirms that miR-29a-3p and miR-192-5p are liver cancer risk (prognosis) related tumor markers. The markers and detection reagents can be used for preparing the rapid-detection pPCR kit, and the kit has the characteristics of rapidness and convenience in detection, high accuracy rate, no wound and the like; a prognostic model built through combination of miR-29a-3p, miR-192-5p and BCLC stage (Barcelona clinic liver cancer stage) can be applied to classification of risk degrees of the liver cancer patients, thereby guiding individualized treatment of the liver cancer patients.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a serum miRNA detection product and the application of miR-29a-3p and miR-192-5p as liver cancer risk (prognosis) tumor molecular markers. Background technique [0002] Liver cancer is the second most common malignant tumor in my country. Because it is a country with a large number of hepatitis B infections, there are many new patients every year, accounting for about 50% of the world's new patients. The existing TNM staging and alpha-fetoprotein (AFP) cannot guide the prognosis of patients well. Many early-stage patients are prone to recurrence and metastasis even after surgery. There is an urgent need for new molecular markers to guide the prognosis of patients and achieve risk classification and stratification. purpose to guide individualized treatment. [0003] Tumor markers (tumormarkers, TM) refer to those synthesized and secreted by the gene expression of tumor cells...

AI Technical Summary

Problems solved by technology

[0007] The content in serum is far lower than that in tissues, requiring a very sensitive and specific detection method; and because the primer sequence is short, primer design is difficult, there is no targeted software that can be used directly, and there is no labeled internal reference gene as a reference, so Primers, reaction systems and internal reference systems all need to be screened and optimized

At present, high-throughput screening for miRNA generally uses chip technology, which has high cost, limited specificity and sensitivity, and cannot meet the needs of real-time detection of scattered samples.

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