Trace amount microorganism quick detection system

A detection system and microorganism technology, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of inaccurate measurement, limited application range, insufficient detection speed or detection accuracy, etc., and achieve the effect of fast and rapid automatic detection

Active Publication Date: 2016-06-15
SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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Problems solved by technology

Flow cytometry has the advantages of fast speed and multi-parameter detection, but requires the concentration of the analyte to be on the order of 10^2 to 10^6, and it cannot be correctly measured for trace microorganisms
[0004

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Embodiment Construction

[0019] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the described specific embodiments are only for explaining the present invention, and are not intended to limit the present invention.

[0020] like figure 1 As shown, the present invention is a rapid detection system for trace microorganisms, including a scanning unit and a control unit.

[0021] The scanning optical path unit includes a laser 1, a beam expander 2, a first beam 3, a first set of filters 41, a second set of filters 42, a dichroic mirror 5, a two-dimensional scanning galvanometer 6, Scan lens 7 , lens 9 , second diaphragm 10 and photodetector 11 .

[0022] The wavelength of the laser 1 matches the fluorescent markers used by the tested microorganisms, and the laser wavelength emitted by the laser 1 is in the range of 200-900nm.

[0023] The beam expander 2 is used to expand the light beam,...

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Abstract

The invention discloses a trace amount microorganism quick detection system which includes a scanning light path unit and a control unit. The scanning light path unit includes a laser device, a beam expanding lens, a first grating, a first light filter set, a second light filter set, a dichroic mirror, a two-dimensional scanning galvanometer, a scanning lens, a lens, a second grating and a photoelectric detector. The scanning light path unit is used for filtering incident laser, focusing light beam, moving a light spot, separating the incident laser and a fluorescent signal, and converting a photoelectric signal. The control unit includes a two-dimensional galvanometer control card, a data collection card and a terminal controller. The data collection card converts an analogue electric signal, which is outputted to the photoelectric detector, into a digital electric signal, and sends data to the terminal controller for processing the data, thereby finally displaying a two-dimensional image. The system is mainly used for detecting and counting fluorescently-dyed microorganisms attached to the surface of a micropore filter membrane and achieves the quick scanning detection on the microorganisms in trace amount.

Description

technical field [0001] The invention relates to a rapid detection system for microorganisms, in particular to a rapid and real-time detection imaging system for trace microorganisms. Background technique [0002] With the rapid development of society, in the fields of industrial production, pharmaceutical water production, food and drug monitoring, etc., it is urgent to realize the rapid and real-time detection of trace bacteria, so as to ensure the quality of industrial production of electronic components, drugs, water quality and food quality and safety. Therefore, in the detection of microorganisms, in addition to the detection of a large number of microorganisms, higher requirements are also put forward for the high-resolution and rapid detection of trace microorganisms. [0003] Among the current rapid detection methods for microorganisms, flow fluorescence microscopy, laser scanning confocal microscopy and flow cytometry are relatively mature, and all have imaging fun...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 李抄毛佳文陈锋顾彪吴太虎杜耀华李玲君
Owner SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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