Synthesis and application of selenized fenugreek polysaccharides

A technology of fenugreek and polysaccharides, which is applied in the medical use of selenized fenugreek polysaccharides and the synthesis field of selenized fenugreek polysaccharides, can solve unreported problems, achieve the goals of expanding the application range, simplifying the preparation, and shortening the time required for the reaction Effect

Active Publication Date: 2016-06-29
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By consulting domestic and foreign literature and patents, there is no report on the method and application of using fenugreek polysaccharide as raw material and ionic liquid as a catalyst to synthesize selenized fenugreek polysaccharide with high selenium content

Method used

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  • Synthesis and application of selenized fenugreek polysaccharides
  • Synthesis and application of selenized fenugreek polysaccharides
  • Synthesis and application of selenized fenugreek polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation of fenugreek polysaccharide formamide solution: Dissolve 200 mg of fenugreek polysaccharide in 60 mL of formamide at 70°C and 500 rpm for 2 hours to obtain a formamide solution of fenugreek polysaccharide.

[0036] (2) Preparation of ionic liquid: Add 5 mL of concentrated sulfuric acid to 10 mL of N-methylpyrrolidone at a rate of 60 drops / min at -10°C, and stir for 12 hours at 800 rpm. After the reaction is completed, use 15 mL of ethyl acetate at room temperature. The ester was washed twice, the lower viscous liquid was collected, and the ionic liquid was obtained by vacuum drying at a vacuum degree of 0.07 MPa and 60° C. for 6 hours.

[0037] (3) Activation of selenous acid by ionic liquid: at 80℃ and N 2 Under protected conditions, 0.6 g of selenous acid was added to 4 mL of ionic liquid to react for 3 hours to obtain activated selenous acid.

[0038] (4) Synthesis of selenized fenugreek polysaccharide: 60mL of fenugreek polysaccharide in formamide solution ...

Embodiment 2

[0050] (1) Preparation of fenugreek polysaccharide formamide solution: 250 mg of fenugreek polysaccharide was dissolved in 100 mL of formamide under stirring at 80°C and 300 rpm for 4 hours to obtain a formamide solution of fenugreek polysaccharide.

[0051] (2) Preparation of ionic liquid: 4mL concentrated sulfuric acid was added dropwise to 10mL N-methylpyrrolidone at a rate of 50 drops / min at 0℃, stirred at 600rpm for 18h, and after the reaction was completed, 7mL ethyl acetate was used at room temperature Washing 3 times, collecting the lower viscous liquid, vacuum drying at a vacuum degree of 0.06MPa and 70°C for 8 hours to obtain an ionic liquid.

[0052] (3) Activation of selenous acid by ionic liquid: at 60℃ and N 2 Under protected conditions, 0.8 g of selenous acid was added to 4 mL of ionic liquid to react for 2 hours to obtain activated selenous acid.

[0053] (4) The synthesis of selenized fenugreek polysaccharide: 100mL of fenugreek polysaccharide in formamide solution w...

Embodiment 3

[0056] (1) Preparation of fenugreek polysaccharide formamide solution: Dissolve 160 mg of fenugreek polysaccharide in 40 mL of formamide at 60°C and 400 rpm for 5 hours to obtain a formamide solution of fenugreek polysaccharide.

[0057] (2) Preparation of ionic liquid: 5mL concentrated sulfuric acid was added dropwise to 10mL N-methylpyrrolidone at a rate of 80 drops / min at -5°C, stirred at 300rpm and reacted for 10h. After the reaction, 15mL ethyl acetate was used at room temperature. The ester was washed 3 times, the lower viscous liquid was collected, and the ionic liquid was obtained by vacuum drying at a vacuum degree of 0.08MPa and 50°C for 12h.

[0058] (3) Activation of selenous acid by ionic liquid: at 70℃ and N 2 Under protected conditions, 0.6 g of selenous acid was added to 4 mL of ionic liquid to react for 1 h to obtain activated selenous acid.

[0059] (4) Synthesis of selenized fenugreek polysaccharide: 40mL of fenugreek polysaccharide in formamide solution was added ...

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Abstract

The invention discloses a method for synthesizing selenized fenugreek polysaccharides. The method comprises the following steps: preparing a fenugreek polysaccharide formamide solution; preparing an ionic liquid; activating selenous acid by using the ionic liquid; and synthesizing the selenized fenugreek polysaccharides. The ionic liquid is utilized to activate the selenous acid to prepare the high-activity selenization reagent, and the bioactive selenized fenugreek polysaccharides are efficiently synthesized under mild conditions. The selenium content is up to 27554-29773 mu g / g. The reagent is friendly to the environment and simple to prepare, does not need any toxic seleniferous intermediate, and does not have the waste liquid discharge, thereby effectively shortening the reaction required lime, and widening the application range of the fenugreek polysaccharides as natural active substances. The activity test indicates that the synthesized selenized fenugreek polysaccharides have obvious in-vitro inhibiting actions on HepG2 liver cancer cells.

Description

Technical field [0001] The present invention belongs to the technical field of biopolymers and chemical synthesis, and relates to a method for synthesizing selenized fenugreek polysaccharide; the present invention also relates to the medical use of selenized fenugreek polysaccharide-as an inhibitor of HepG2 liver cancer cells for the preparation of anti- Cancer drugs. Background technique [0002] Selenium (Se) is an indispensable trace element for the human body, and it plays an important role in improving the immune function of life, anti-cancer, anti-oxidation, and preventing nutritional liver necrosis. In biological metabolism, selenium is also the active component of glutathione peroxidase. Therefore, selenium is extremely important to the human body. However, selenium is unevenly distributed on the earth. About three-fifths of China is lacking in selenium. The content of selenium in ordinary food is extremely low, and the effect of selenium supplementation is generally no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P1/16
CPCA61K31/715C08B37/00
Inventor 王俊龙杨文包爱娟吴婷李妹妹杨晓萍曾俊媛梁俊玉孔维宝姚健张继
Owner NORTHWEST NORMAL UNIVERSITY
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