Bacillus tequilensis and application thereof

A technology of Bacillus tequila and Bacillus, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of destroying the fermentation process, unfavorable health, and affecting the flavor of the sauce

Active Publication Date: 2016-07-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of fermenting and preparing broad bean paste, the breeding of harmful microorganisms in the fermented soybean paste often

Method used

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  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The screening of embodiment 1 Tequila bacillus

[0018] Take 1g sample from the traditional fermented bean paste fermented bean paste for about 60 days, add it to 9mL sterile water, shake slightly to make a suspension, dilute the suspension gradiently, spread it on LB plates, and incubate aerobically at 37°C for 24 to 48h. Pick the white flat and wrinkled single colony and separate it by streaking on the plate repeatedly, and repeat three times to obtain a purified single colony for the next step of identification.

[0019] The identification is mainly through: 1) Observation of colony characteristics: directly observe with the naked eye, and select strains with a diameter of 2 to 4 mm, round or irregular, rough surface with wrinkles, white and opaque, etc. that meet the characteristics of Bacillus tequila colonies. 2) Observation of individual morphology: Observation by Gram staining with an optical microscope. Pick a rod-shaped, Gram-positive strain. Strains meetin...

Embodiment 2

[0021] The inhibition of bacillus tequila described in embodiment 2 to bacillus cereus in fermented sauce (filter paper method)

[0022] The medium used in this experiment is LB medium: peptone 1%; yeast powder 0.5%; NaCl 1%; pH natural. Divided into Erlenmeyer flasks, the filling volume is 40mL, and sterilized at 121°C for 20min. The solid LB agar content was 2%.

[0023] The -80°C glycerol stock solution was inoculated into fresh LB liquid medium with a 1% inoculum amount, and cultured on a shaker at 37°C for 24 hours at a rotation speed of 180 rpm. Then subculture with 2% inoculation amount, and cultivate for about 24 hours and can be used as experimental bacteria. The activation and subculture process of Bacillus cereus used in the experiment was the same as above. The bacteria concentrations of the experimental bacteria (Bacillus tequila) and the indicator bacteria (Bacillus cereus) after passage were all at 10 8 About CFU / mL. Dilute the indicator bacteria to 10 6 L...

Embodiment 3

[0030] Embodiment 3 is applied to traditional fermented broad bean paste

[0031] CGMCC No. 12092 Bacillustequilensis HYM24 strain was cultured in LB liquid medium for 1 day as seed solution. The seed liquid was inoculated into the sauce unstrained spirits, and the number of Bacillus cereus changed significantly during the fermentation process. The control was the sauce unstrained spirits not inoculated with the seed liquid, and a large number of waxy Bacillus (about 2.9×10 4 CFU / g), while the number of Bacillus cereus in the inoculated sauce body was maintained at a low level (about 2.01×10 2 CFU / g).

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Abstract

The invention discloses bacillus tequilensis and application thereof, and belongs to the technical field of fermenting.The bacillus tequilensis was stored in CGMCC on January 21, 2016 with the file No.12092.The storage address is Institute of Microbiology Chinese Academy of Sciences, No.3, 1st Yard, Beichen West Rd, Chaoyang District, Beijing City.The bacillus tequilensis strain has high anti-bacteria performance, and is capable of effectively controlling growth of bacillus cereus in fermenting of broad-bean sauce, and bacillus cereus is a latent pathogenic bacteria.

Description

technical field [0001] The invention relates to a strain of bacillus tequila and application thereof, belonging to the technical field of fermentation. Background technique [0002] The thallus of Bacillus is rod-shaped, long straight or nearly straight, and the size is 0.3-2.2×1.2-7.0 microns. Mostly motile, flagella typically lateral. The bacteria can form heat-resistant endospores, and there is no more than one spore in one sporangia cell. The exposure of the bacteria to the air does not hinder the formation of spores, and the Gram reaction is positive, but it may appear negative only in the early growth period. Organic chemical energy nutrition can use a variety of substrates to carry out strict respiratory metabolism, strict fermentation metabolism or both respiratory and fermentation metabolism. In respiratory metabolism, the ultimate electron acceptor is molecular oxygen, which can be replaced by nitrate in some species, and most species can produce hydroxide enzym...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L29/00C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 李崎朱林江范子豪刘春凤郑飞云王金晶李永仙王佳琪
Owner JIANGNAN UNIV
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