Kit of detecting D-3-hydroxybutyric acid through enzymic method and preparation method thereof

A D-3-, kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of bilirubin interference, influence detection, negative values, etc., and achieve anti-bilirubin interference. The performance enhancement, the clinical application value and the sensitivity improvement effect

CN105734113AActive Publication Date: 2016-07-06SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2016-07-06

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Abstract

The invention provides a kit of detecting D-3-hydroxybutyric acid through an enzymic method. The kit is composed of a first reagent and a second reagent according to the volume part ratio of 3:1. 3L of the first reagent includes 10-200 mmol / L of a buffer liquid, 50-200 U / L of a D-3-hydroxybutyric dehydrogenase, 1-3 g / L of Brij35 (dodecyl polyglycol ether), 0.1-2 g / L of sodium azide, and deionized water being added to the components until the total volume is 3L; and 1L of the second reagent includes: 10-200 mmol / L of oxalate, 0.1-10 mmol / L of 3-acetylpyridine nicotinamide adenine dinucleotide, 0.1-2 g / L of sodium azide, and deionized water being added to the components until the total volume is 1L. The kit has strong performance of resisting bilirubin interference, has high reagent sensitivity, is improved in detection accuracy and has great clinical application value.
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Description

technical field

[0001] The invention relates to biological reagents, in particular to a detection kit, in particular to a kit for enzymatic detection of D-3-hydroxybutyric acid and a preparation method thereof. Background technique

[0002] Diabetic ketoacidosis is a common clinical disease, frequently-occurring disease, caused by increased blood ketones, D-3-hydroxybutyric acid is the main component of ketones in the blood (accounting for 78%), it can reflect the ketones in the blood generated situation. When ketoacidosis occurs, D-3-hydroxybutyric acid is significantly increased. Therefore, D-3-hydroxybutyric acid can be used for early diagnosis and treatment monitoring of ketoacidosis, and can guide the treatment and efficacy monitoring of ketoacidosis . Because in ketoacidosis, the patient has an antagonistic effect on insulin, resulting in protein synthesis disorder and protein degradation metabolism, therefore, by measuring the fasting blood sugar in the morning, it ...

Examples

Embodiment 1

[0028] Example 1 Preparation of D-3-hydroxybutyric acid detection kit

[0029] composition:

[0030] Reagent 1: (3L) (reagent volume)

[0031]

[0032] Reagent 2: (1L) (reagent volume)

[0033]

[0034]

[0035] preparation:

[0036] (1) Preparation of reagent 1: (3L)

[0037] (1) First add 2.4L of deionized water to the container;

[0038] (2) Tris buffer solution, lauryl polyethylene glycol ether, sodium azide are added successively;

[0039] (3) Add enzyme: D-3-hydroxybutyrate dehydrogenase

[0040] (4) Finally, add deionized water until the total volume is 3L and mix evenly to obtain;

[0041] (2) Preparation of reagent 2: (1L)

[0042] Add oxalate, 3-acetylpyridine oxidized coenzyme, and sodium azide deionized water to the container in sequence until the total volume is 1 L, and mix well.

Embodiment 2

[0044] Reagent 1: (3L) (reagent volume)

[0045]

[0046] Reagent 2: (1L) (reagent volume)

[0047]

[0048]

[0049] The preparation method is the same as in Example 1.

Embodiment 3

[0051] Reagent 1: (3L) (reagent volume)

[0052]

[0053] Reagent 2: (1L) (reagent volume)

[0054]

[0055] Preparation method is the same as embodiment 1