Strawberry anthocyanin regulatory gene functional marker

A technology for regulating genes and anthocyanins, which is applied in the fields of fruit tree breeding and molecular genetics, can solve the problems of molecular markers that have not been reported yet and few molecular markers, and achieve the effects of improving breeding efficiency, high specificity, and good repeatability

Inactive Publication Date: 2016-07-06
SHENYANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few molecular markers related to strawberry color at present, a

Method used

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  • Strawberry anthocyanin regulatory gene functional marker
  • Strawberry anthocyanin regulatory gene functional marker
  • Strawberry anthocyanin regulatory gene functional marker

Examples

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Effect test

example 1

[0030] Example 1 utilizes functional marker Fv-RY to F 2 Early color identification of individual strawberry plants isolated from populations

[0031] (1) Genomic DNA of red and yellow strawberry seedlings was extracted by CTAB method, and the extraction steps were as follows:

[0032] A. Take 0.2g of fresh young leaves with the main veins removed, place them in a 2mL centrifuge tube, put them into liquid nitrogen for quick freezing, and quickly grind them to powder with a clean plastic pestle cleaned with 75% alcohol;

[0033] B. Add 600 μL of CTAB extract (CTAB2%, Tris-HCl (PH8.0), 100mmol / L, EDTA20mmol / L, NaCl1.4mol / L) preheated at 65°C to the centrifuge tube, and mix quickly;

[0034] C. Then put the centrifuge tube into a water bath at 65°C, shake it every 8 minutes, and bathe in water for 30 minutes;

[0035] D. Take out the centrifuge tube, add an equal volume of chloroform, shake well for 10 minutes, and then centrifuge at 12000r / min at 4°C for 10 minutes;

[0036] ...

example 2

[0054] Example 2 Identification of Strawberry Varieties and Lines of Different Colors Using Functional Marker Fv-RY

[0055] (1) Using the CTAB method to extract genomic DNA from the young leaves of strawberry varieties and strains of different colors, the extraction steps are as follows:

[0056] A. Take 0.2g of fresh young leaves with the main veins removed, place them in a 2mL centrifuge tube, put them into liquid nitrogen for quick freezing, and quickly grind them to powder with a clean plastic pestle cleaned with 75% alcohol;

[0057] B. Add 600 μL of CTAB extract (CTAB2%, Tris-HCl (PH8.0), 100mmol / L, EDTA20mmol / L, NaCl1.4mol / L) preheated at 65°C to the centrifuge tube, and mix quickly;

[0058] C. Then put the centrifuge tube into a water bath at 65°C, shake it every 8 minutes, and bathe in water for 30 minutes;

[0059] D. Take out the centrifuge tube, add an equal volume of chloroform, shake well for 10 minutes, and then centrifuge at 12000r / min at 4°C for 10 minutes;...

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Abstract

The invention provides a strawberry anthocyanin regulatory gene functional marker and belongs to the fields of fruit tree breeding and molecular genetics. The strawberry anthocyanin regulatory gene functional marker is characterized in that one mononucleotide mutation exists in coding regions by carrying out sequence comparison on a strawberry anthocyanin regulatory gene MYB10 in red and yellow strawberries, a dCAPS marker Fv-Ry is designed according to difference of the mononucleotide, the dCAPS marker can distinguish yellow, heterozygous red and homozygous red strawberries, and the marker and strawberry fruit colour character are in cosegregation. By utilizing the functional marker, a material carrying an anthocyanin regulatory gene can be rapidly and accurately screened and identified from a large number of strawberry germplasm resources, molecular marker early selective breeding is carried out, and progress of strawberry fruit colour breeding is greatly accelerated.

Description

technical field [0001] The invention belongs to the field of fruit tree breeding and molecular genetics, and provides a strawberry anthocyanin regulatory gene function marker. Background technique [0002] Strawberry (Fragaria × ananassa Duch.) is a plant of the genus Fragaria in the Rosaceae family. It is very popular because of its bright or gorgeous color, high nutritional value and delicious taste. At present, the cultivation area of ​​strawberries in my country exceeds 2 million mu. Bright or beautiful colors can enhance the market competitiveness of products and directly affect the economic benefits of strawberry growers. However, the color of strawberry fruit can only be shown at the end of strawberry maturity, which greatly delays the breeding process. Therefore, in order to speed up the color breeding of strawberry fruit, it is necessary to carry out molecular marker-assisted breeding by using modern molecular biotechnology. At present, some scholars have carried...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2565/125C12Q2531/113
Inventor 张俊祥张志宏石伟佳张豫超
Owner SHENYANG AGRI UNIV
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