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A kind of Trichoderma chrysanthemum strain and the method for using it to prepare frangipani tobacco spices

A technology for tobacco flavors and Trichoderma yellow green, which is applied in the directions of preparing tobacco, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of increasing aroma richness, realizing large-scale industrial production, and having good application prospects.

Active Publication Date: 2019-01-15
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on the use of Trichoderma chrysanthemum for the fermentation of frangipani to prepare tobacco spices

Method used

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  • A kind of Trichoderma chrysanthemum strain and the method for using it to prepare frangipani tobacco spices
  • A kind of Trichoderma chrysanthemum strain and the method for using it to prepare frangipani tobacco spices

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Embodiment 1

[0023] 1. Optimal conditions for enzyme production of Trichoderma chrysogreen strain HLMM-04

[0024] 1.1 Effect of initial pH of medium on enzyme production of HLMM-04 strain

[0025] The pH of the liquid medium was adjusted to 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 respectively, and after shaking culture at 28°C and 120rpm for 3 days, the cellulase activity was measured, showing that the optimum initial pH was 6.5.

[0026] 1.2 Effect of culture temperature on enzyme production of HLMM-04 strain

[0027] Place the inoculated liquid fermentation medium at 20°C, 25°C, 28°C, 32°C, and 35°C respectively, and culture with shaking at 120r / min for 3 days, then measure the cellulase activity, which shows that the most suitable culture temperature is 28°C .

[0028] 1.3 Effect of culture time on enzyme production of HLMM-04 strain

[0029] The inoculated liquid medium (pH 6.5) was placed in a constant temperature and humidity chamber, 28°C, 120r / min shaking culture, and the cellulase a...

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Abstract

The invention discloses a Trichoderma chrysanthemum strain and a method for preparing frangipani tobacco spices by using it. The strain is Trichoderma aureoviride HLMM-04 CGMCC No.10839, which has the ability to produce cellulase, and then promote the decomposition of cellulose into oligosaccharides or monosaccharides. The cellulase crude enzyme solution is obtained by activating, fermenting, filtering and dialysis of the bacterial strain, which is used to ferment frangipani, and then is extracted, filtered and concentrated under reduced pressure to make flavor for tobacco. The optimum conditions for enzyme production of HLMM‑04 strain were that the initial pH of the medium was 6.5, the culture temperature was 28°C, and the culture time was 5 days. The results of cigarette flavoring showed that the prepared spices could enrich the aroma and increase the sweetness of cigarettes.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and its application, and specifically relates to a new strain of Trichoderma chrysogreens HLMM-04, and at the same time, the invention also relates to a method for preparing frangipani tobacco spices by using the strain. Background technique [0002] Flavoring is a key link in the cigarette process, and it is one of the effective means to effectively improve the smoking quality of cigarettes and form a unique style of cigarettes. At present, most of the tobacco flavors added to tobacco in my country are extracted by traditional methods and then blended. Although the production and operation are simple, there are also disadvantages such as monotonous aroma and ineffective improvement of cigarette smoking. The use of microorganisms to ferment aroma raw materials can increase the content of various aroma substances in traditionally extracted spices, enhance the richness of aroma, and then produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02A24B3/12C12R1/885
CPCA24B3/12C12P1/02C12N1/145C12R2001/885
Inventor 李源栋党立志段焰青王凯蒋举兴高莉周兴奎
Owner CHINA TOBACCO YUNNAN IND
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